CuGenDBv2

Gene ID	IVF0019974
Organism	Cucumis melo ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Pyruvate kinase
Genome location	chr11:22982090..22982742
RNA-Seq Expression	IVF0019974
Synteny	IVF0019974
Gene Ontology terms	GO:0006096 - glycolytic process (biological process) GO:0009570 - chloroplast stroma (cellular component) GO:0000287 - magnesium ion binding (molecular function) GO:0004743 - pyruvate kinase activity (molecular function) GO:0005524 - ATP binding (molecular function) GO:0016301 - kinase activity (molecular function) GO:0030955 - potassium ion binding (molecular function)
InterPro domains	IPR001697 - Pyruvate kinase IPR015793 - Pyruvate kinase, barrel IPR015806 - Pyruvate kinase, insert domain superfamily IPR015813 - Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily IPR040442 - Pyruvate kinase-like domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0038540.1 pyruvate kinase isozyme G [Cucumis melo var. makuwa]

7.27e-41

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DHSSH+KTIDLVKEYNAQFNDKVIAIMLDTKG +VRS DVPKPILLKEGQEFNFTIKRGV +E    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

RVW50022.1 Pyruvate kinase isozyme G, chloroplastic [Vitis vinifera]

1.22e-42

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DH+SHKKTIDLVKEYNAQF DKVIAIMLDTKG +VRS DVPKPI+LKEGQEFNFTIKRGV SE    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

RVX14345.1 Pyruvate kinase isozyme G, chloroplastic [Vitis vinifera]

6.04e-43

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DH+SHKKTIDLVKEYNAQF DKVIAIMLDTKG +VRS DVPKPI+LKEGQEFNFTIKRGV SE    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

XP_008466644.1 PREDICTED: pyruvate kinase isozyme G, chloroplastic [Cucumis melo]

1.21e-40

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DHSSH+KTIDLVKEYNAQFNDKVIAIMLDTKG +VRS DVPKPILLKEGQEFNFTI+RGV +E    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

XP_010663632.1 PREDICTED: pyruvate kinase isozyme G, chloroplastic isoform X2 [Vitis vinifera]

1.69e-40

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DH+SHKKTIDLVKEYNAQF DKVIAIMLDTKG +VRS DVPKPI+LKEGQEFNFTIKRGV SE    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CRS5 Pyruvate kinase

3.7e-33

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DHSSH+KTIDLVKEYNAQFNDKVIAIMLDTKG +VRS DVPKPILLKEGQEFNFTI+RGV +E    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

A0A438EQQ7 Pyruvate kinase

4.8e-33

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DH+SHKKTIDLVKEYNAQF DKVIAIMLDTKG +VRS DVPKPI+LKEGQEFNFTIKRGV SE    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

A0A5A7T5H5 Pyruvate kinase

1.7e-33

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DHSSH+KTIDLVKEYNAQFNDKVIAIMLDTKG +VRS DVPKPILLKEGQEFNFTIKRGV +E    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

A0A5D3E4W6 Pyruvate kinase

3.7e-33

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DHSSH+KTIDLVKEYNAQFNDKVIAIMLDTKG +VRS DVPKPILLKEGQEFNFTI+RGV +E    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

F6HTA0 Pyruvate kinase

4.8e-33

78.95

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  ETGMNVARLNMSH DH+SHKKTIDLVKEYNAQF DKVIAIMLDTKG +VRS DVPKPI+LKEGQEFNFTIKRGV SE    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

SwissProt top hits

e value

%identity

Alignment

P55964 Pyruvate kinase isozyme G, chloroplastic (Fragment)

1.9e-10

56.67

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Query:  NAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        NAQ +D V++IMLDTKG +VRS DVP+P +LKEGQEFN TI+RGV ++    +   D  N
Subjt:  NAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

Q40546 Pyruvate kinase isozyme G, chloroplastic

1.4e-32

73.68

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  E GMNVARLNMSH DH+SH++TIDLVKEYNAQF DKVIAIMLDTKG +V S DVPKPILLKEGQEFNF+IKRGV +E    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

Q93Z53 Plastidial pyruvate kinase 3, chloroplastic

2.4e-29

68.42

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  E GMNVARLNMSH DH+SH+ TIDLVKEYN+ F DK IAIMLDTKG +VRS DVP+PI L+EGQEFNFTIKRGV  +    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

Q9FLW9 Plastidial pyruvate kinase 2

4.5e-28

64.21

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  E GMNVAR+NMSH DH+SHKK IDLVKEYNAQ  D  IAIMLDTKG +VRS D+P+PI+L  GQEF FTI+RGV + +   +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

Q9PK61 Pyruvate kinase

2.7e-09

45.45

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIK
        M+ K  + GMNVARLN SH  H SH +TI ++KE   +     +AIMLDTKG ++R   V  PI +K G     T K
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIK

Arabidopsis top hits

e value

%identity

Alignment

AT1G32440.1 plastidial pyruvate kinase 3

1.7e-30

68.42

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  E GMNVARLNMSH DH+SH+ TIDLVKEYN+ F DK IAIMLDTKG +VRS DVP+PI L+EGQEFNFTIKRGV  +    +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

AT3G04050.1 Pyruvate kinase family protein

2.6e-07

41.03

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKV-IAIMLDTKGLKVRSEDVP--KPILLKEGQEFNFT
        M+ K  + GMNVAR N SH  HS H++T+D ++   A  N  +  A+MLDTKG ++R+  +   KP+ L +GQE   +
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKV-IAIMLDTKGLKVRSEDVP--KPILLKEGQEFNFT

AT3G25960.1 Pyruvate kinase family protein

8.2e-09

40.23

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVI-AIMLDTKGLKVRSEDVP--KPILLKEGQEFNFTIKRGVKSEA
        MI K  + GMNVAR N SH  HS H++T+D ++   A  N  ++ A+MLDTK   +R+  +   KPI LK+GQE   +I   ++ ++
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVI-AIMLDTKGLKVRSEDVP--KPILLKEGQEFNFTIKRGVKSEA

AT3G55650.1 Pyruvate kinase family protein

3.7e-09

40.23

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVI-AIMLDTKGLKVRSEDVP--KPILLKEGQEFNFTIKRGVKSEA
        MI K  + GMNVAR N SH  HS H++T+D ++   A  N  ++ A+MLDTKG ++R+  +   KPI L +GQE   +I   ++ ++
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVI-AIMLDTKGLKVRSEDVP--KPILLKEGQEFNFTIKRGVKSEA

AT5G52920.1 plastidic pyruvate kinase beta subunit 1

3.2e-29

64.21

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Query:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN
        MIWK  E GMNVAR+NMSH DH+SHKK IDLVKEYNAQ  D  IAIMLDTKG +VRS D+P+PI+L  GQEF FTI+RGV + +   +   D  N
Subjt:  MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQN

Sequences

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CDS sequence

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ATGATATGGAAATCGACAGAGACTGGGATGAATGTAGCACGTTTAAATATGTCCCATGAAGACCATTCTTCCCATAAGAAAACAATTGATTTGGTTAAGGAATATAACGC
CCAATTTAATGACAAAGTTATAGCCATTATGCTTGATACGAAGGGTCTTAAGGTTCGAAGTGAAGATGTACCTAAACCAATCTTGCTCAAAGAGGGACAAGAATTTAACT
TCACAATCAAAAGAGGAGTCAAATCTGAGGCAGTTTGGAGACTAAAATGCTCTGACATACAAAATTTATGTGCTTGGGTTTGGCCAATGGTTGTCTTCTGA

mRNA sequence

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Protein sequence

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MIWKSTETGMNVARLNMSHEDHSSHKKTIDLVKEYNAQFNDKVIAIMLDTKGLKVRSEDVPKPILLKEGQEFNFTIKRGVKSEAVWRLKCSDIQNLCAWVWPMVVF

IVF0019974 (gene) of Melon (IVF77) v1 genome