CuGenDBv2

Gene ID	Lag0000656
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Histidine--tRNA ligase
Genome location	chr4:12164786..12169711
RNA-Seq Expression	Lag0000656
Synteny	Lag0000656
Gene Ontology terms	GO:0000166 - nucleotide binding (molecular function) GO:0004812 - aminoacyl-tRNA ligase activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6604395.1 Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

1.5e-12

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

KAG7034545.1 Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.5e-12

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

XP_022978407.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima]

1.5e-12

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

XP_023544232.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo]

1.5e-12

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

XP_023544233.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo]

1.5e-12

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

TrEMBL top hits

e value

%identity

Alignment

A0A5D3C5Z0 Histidine--tRNA ligase

2.7e-12

64.29

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +R+GIT  DVGFKVSSRKVLQ+VLKCYGIP+N+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

A0A6J1ECF7 Histidine--tRNA ligase

7.1e-13

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

A0A6J1EG18 Histidine--tRNA ligase

7.1e-13

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

A0A6J1IL10 Histidine--tRNA ligase

7.1e-13

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

A0A6J1IMK6 Histidine--tRNA ligase

7.1e-13

67.86

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIG+T  DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

SwissProt top hits

e value

%identity

Alignment

O82413 Histidine--tRNA ligase, chloroplastic/mitochondrial

4.0e-13

62.5

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIGIT  DVGFKVSSRKVLQE+LK YG+PE+LFG+VCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

Arabidopsis top hits

e value

%identity

Alignment

AT3G46100.1 Histidyl-tRNA synthetase 1

2.8e-14

62.5

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Query:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        + +L        +RIGIT  DVGFKVSSRKVLQE+LK YG+PE+LFG+VCI IDK+
Subjt:  QLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

AT5G03406.1 Class II aaRS and biotin synthetases superfamily protein

2.4e-13

77.27

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Query:  RRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV
        +RIGIT  DVGFKVSSRKVLQE LK YG+PE+LFG+VC  IDKV
Subjt:  RRIGITVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKV

Sequences

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CDS sequence

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ATGAGGAGGGGGAAAGCGACAGTAGAGAGGGGGGATAAATCAGAGCATGAAAAATCTGGAGAGGATATAGACGATAATGATGGAAAGGGTAGAAATGAACAACAACAAAC
TCCAGTTACGATGCTGATGGAGGATGATATTGTCAGCACAAACTTCCTCAAAGAGGTTGAAAAGATTGAACATGAAGCAAAGGTCTTAAAGGCAAAAAAACAAACTGTGA
AGAAAGAAGTTGAAAATATAGTCGATAACGCATACATTCATGCTGTGTTTCGTTTAATATTATACATGTTCAGATCTATTCATCATGGGTTTTTGTGTGTCGTCTCATAT
CTGACAGACACCTTCGAATGCACTAGCGTATATTGTGACTTGAGGGTTTCAAATCTCATCGCCATTTCGAGCATGTTCACCTTGCCAAAGATTGCCATTAGGATGCCCCG
TGATTCAATTCGTGAACTTGAATCGCAACTAGTTCAAATCTTGGGTGGTTTGGGTGGTTGGTGTTGGAGTTGGTCTTCACCACTTTGTTTCCTAAACCAGTTGGACCTTG
GAGAACGATTTGCAGTGGTTATTAGACGAATTGGAATTACAGTCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATA
CCTGAAAATCTGTTTGGCAAGGTTTGCATATTCATAGACAAGGTTCTGTCCAGCAGCGAGTACGAACAAACCAGAGCTCAAAACGACGATCCAACCGTTCAAAACGAGGC
CCTATGTGTCCCCAACAAGCTGACAAAATTTCAGCACAATCCAACGGTTAAAACTCCGGCAATCGACAACTTTGTGAACGCTGTCGCTGAAAATCCGAACTGCACTGCTC
TCCCTGTTTCCCTCCGTTTTTCTGTGTAA

mRNA sequence

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Protein sequence

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MRRGKATVERGDKSEHEKSGEDIDDNDGKGRNEQQQTPVTMLMEDDIVSTNFLKEVEKIEHEAKVLKAKKQTVKKEVENIVDNAYIHAVFRLILYMFRSIHHGFLCVVSY
LTDTFECTSVYCDLRVSNLIAISSMFTLPKIAIRMPRDSIRELESQLVQILGGLGGWCWSWSSPLCFLNQLDLGERFAVVIRRIGITVPDVGFKVSSRKVLQEVLKCYGI
PENLFGKVCIFIDKVLSSSEYEQTRAQNDDPTVQNEALCVPNKLTKFQHNPTVKTPAIDNFVNAVAENPNCTALPVSLRFSV

Lag0000656 (gene) of Sponge gourd (AG-4) v1 genome