CuGenDBv2

Gene ID	Lag0000760
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase
Genome location	chr4:15413624..15415682
RNA-Seq Expression	Lag0000760
Synteny	Lag0000760
Gene Ontology terms	GO:0006259 - DNA metabolic process (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0004518 - nuclease activity (molecular function) GO:0008233 - peptidase activity (molecular function) GO:0008270 - zinc ion binding (molecular function) GO:0016779 - nucleotidyltransferase activity (molecular function)
InterPro domains	IPR005162 - Retrotransposon gag domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0035455.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]

1.6e-86

68.25

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Query:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE
        +E+GR +RA PSD EK YGIERLKKLGATVFEGSTDPAD E WLNMLEKCFDVM+CPEERK RLATFLLQKEAEGWWKSILARRS A  LDWQ FRGIFE
Subjt:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE

Query:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------
        DKYYP+TYCEAKRDEFLGLKQGSLSVAEYERK  ELSRYADVI+ASESDRCRRFE+GLRFEIRTP                                   
Subjt:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------

Query:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR
                     RRF PG+N+S RQDFK R GGQ+SR +S GS +QRQSQR
Subjt:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR

KAA0037581.1 reverse transcriptase [Cucumis melo var. makuwa]

2.7e-91

66.31

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Query:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE
        VLAD+ GRIRT   ILP+           E+GRPE+AGPSDLEKMYGIERLKKLGATVFEGSTDPAD EVWLNMLEKCFDVMSCP+ERK +LATFLL KE
Subjt:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE

Query:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------
        AEGWWKSI+ARR+ A  LDWQ FRGIFE+KYYPTTYCEAKRDEFL LKQ SLSVA+YERK  ELSRYA++IVASESDRC RFE+GLRFEIRTP       
Subjt:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------

Query:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS
                                                 RRF PGVNVSG QDFKRR GG+  RQMSSGSAYQRQS+RAS
Subjt:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS

KAA0037805.1 uncharacterized protein E6C27_scaffold918G00190 [Cucumis melo var. makuwa]

1.2e-86

68.25

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Query:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE
        +E+GR +RA PSD EK YGIERLKKLGATVFEGSTDPAD E WLNMLEKCFDVM+CPEERK RLATFLLQKEAEGWWKSILARRS A  LDWQ FRGIFE
Subjt:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE

Query:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------
        DKYYP+TYCEAKRDEFLGLKQGSLSVAEYERK  ELSRYADVI+ASESDRCRRFE+GLRFEIRTP                                   
Subjt:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------

Query:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR
                     RRF PG+N+S RQDFK R GGQ+SR +S GS +QRQSQR
Subjt:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR

KAA0066849.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]

1.6e-86

68.25

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Query:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE
        +E+GR +RA PSD EK YGIERLKKLGATVFEGSTDPAD E WLNMLEKCFDVM+CPEERK RLATFLLQKEAEGWWKSILARRS A  LDWQ FRGIFE
Subjt:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE

Query:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------
        DKYYP+TYCEAKRDEFLGLKQGSLSVAEYERK  ELSRYADVI+ASESDRCRRFE+GLRFEIRTP                                   
Subjt:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------

Query:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR
                     RRF PG+N+S RQDFK R GGQ+SR +S GS +QRQSQR
Subjt:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR

TYK03091.1 reverse transcriptase [Cucumis melo var. makuwa]

4.2e-92

66.67

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Query:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE
        VLAD+ GRIRT   ILP+           E+GRPE+AGPSDLEKMYGIERLKKLGATVFEGSTDPAD EVWLNMLEKCFDVMSCP+ERK +LATFLLQKE
Subjt:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE

Query:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------
        AEGWWKSI+ARR+ A  LDWQ FRGIFE+KYYPTTYCEAKRDEFL LKQ SLSVA+YERK  ELSRYA++IVASESDRC RFE+GLRFEIRTP       
Subjt:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------

Query:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS
                                                 RRF PGVNVSG QDFKRR GG+  RQMSSGSAYQRQS+RAS
Subjt:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS

TrEMBL top hits

e value

%identity

Alignment

A0A5A7T7M6 Reverse transcriptase

1.3e-91

66.31

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Query:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE
        VLAD+ GRIRT   ILP+           E+GRPE+AGPSDLEKMYGIERLKKLGATVFEGSTDPAD EVWLNMLEKCFDVMSCP+ERK +LATFLL KE
Subjt:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE

Query:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------
        AEGWWKSI+ARR+ A  LDWQ FRGIFE+KYYPTTYCEAKRDEFL LKQ SLSVA+YERK  ELSRYA++IVASESDRC RFE+GLRFEIRTP       
Subjt:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------

Query:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS
                                                 RRF PGVNVSG QDFKRR GG+  RQMSSGSAYQRQS+RAS
Subjt:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS

A0A5A7T8K4 Retrotrans_gag domain-containing protein

5.8e-87

68.25

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Query:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE
        +E+GR +RA PSD EK YGIERLKKLGATVFEGSTDPAD E WLNMLEKCFDVM+CPEERK RLATFLLQKEAEGWWKSILARRS A  LDWQ FRGIFE
Subjt:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE

Query:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------
        DKYYP+TYCEAKRDEFLGLKQGSLSVAEYERK  ELSRYADVI+ASESDRCRRFE+GLRFEIRTP                                   
Subjt:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------

Query:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR
                     RRF PG+N+S RQDFK R GGQ+SR +S GS +QRQSQR
Subjt:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR

A0A5A7U2V7 Reverse transcriptase

7.5e-87

68.25

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Query:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE
        +E+GR +RA PSD EK YGIERLKKLGATVFEGSTDPAD E WLNMLEKCFDVM+CPEERK RLATFLLQKEAEGWWKSILARRS A  LDWQ FRGIFE
Subjt:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE

Query:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------
        DKYYP+TYCEAKRDEFLGLKQGSLSVAEYERK  ELSRYADVI+ASESDRCRRFE+GLRFEIRTP                                   
Subjt:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------

Query:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR
                     RRF PG+N+S RQDFK R GGQ+SR +S GS +QRQSQR
Subjt:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR

A0A5D3BS67 Reverse transcriptase

7.5e-87

68.25

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Query:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE
        +E+GR +RA PSD EK YGIERLKKLGATVFEGSTDPAD E WLNMLEKCFDVM+CPEERK RLATFLLQKEAEGWWKSILARRS A  LDWQ FRGIFE
Subjt:  EEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFE

Query:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------
        DKYYP+TYCEAKRDEFLGLKQGSLSVAEYERK  ELSRYADVI+ASESDRCRRFE+GLRFEIRTP                                   
Subjt:  DKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-----------------------------------

Query:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR
                     RRF PG+N+S RQDFK R GGQ+SR +S GS +QRQSQR
Subjt:  -------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQR

A0A5D3BTP3 Reverse transcriptase

2.0e-92

66.67

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Query:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE
        VLAD+ GRIRT   ILP+           E+GRPE+AGPSDLEKMYGIERLKKLGATVFEGSTDPAD EVWLNMLEKCFDVMSCP+ERK +LATFLLQKE
Subjt:  VLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYGIERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKE

Query:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------
        AEGWWKSI+ARR+ A  LDWQ FRGIFE+KYYPTTYCEAKRDEFL LKQ SLSVA+YERK  ELSRYA++IVASESDRC RFE+GLRFEIRTP       
Subjt:  AEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEYERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTP-------

Query:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS
                                                 RRF PGVNVSG QDFKRR GG+  RQMSSGSAYQRQS+RAS
Subjt:  -----------------------------------------RRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRAS

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G43760.1 DNAse I-like superfamily protein

4.5e-07

27.66

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Query:  EIYWKQRSREDWLKWGDRNTKWFHQ-----KASSRRKKIGFEEDV---------NKVLAYVPRRITADMNLMLTS---------PF--------------
        E +++Q+SR  WL+ GD NT++FH+     +A +  K +  ++DV           ++AY    + +D +++            PF              
Subjt:  EIYWKQRSREDWLKWGDRNTKWFHQ-----KASSRRKKIGFEEDV---------NKVLAYVPRRITADMNLMLTS---------PF--------------

Query:  SEEEVLAAVRGFPPSKAPGLDGFPALFYQKYWELVGPTTIS
        S++E+ AAV   P +KAPG D F A F+ + W +V  +TI+
Subjt:  SEEEVLAAVRGFPPSKAPGLDGFPALFYQKYWELVGPTTIS

Sequences

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CDS sequence

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ATGTGGCAACATGAAAAGTTGGGTGGTCCAGATAGAGATATGCGACAAATATCGGCATTTCGAGAAGTATTGGACGAATGCAATTTAAAAGATCTCGACTTTTCGGGGGA
AATGGGCCTCCATGTGTCTAATCTTGACTGGGCGAAGTCTGACCATCGTCAGATTGAGCTTAGTTTGGAGGGAAGGATGTATCGGGAGGTCCAATCCCGCAGTCGTAGAT
CCTTTAAATTTGAAGAATGGTGGACTCGTGATAATAAATGTGGCAATATCATTAGGCATGCCGGCGGTTGGGTGGGAGCTAGAGATTTCACTCAGCCTCTTCATATTGCT
TTGAAGAAGTGTGCTGCAGAATTGGTGCATCATTTGGAGCGTCAGTTGGATTTGTTACTTTTGGAAGAAGAGATCTATTGGAAGCAGCGATCGAGGGAAGATTGGCTCAA
ATGGGGAGATCGTAATACTAAATGGTTTCATCAGAAGGCTTCATCAAGGCGGAAAAAAATTGGGTTTGAGGAAGATGTGAATAAGGTTTTGGCCTATGTTCCTAGGAGGA
TTACTGCGGATATGAATCTGATGCTTACATCCCCGTTTTCGGAGGAGGAAGTGCTTGCAGCTGTGAGAGGTTTTCCTCCATCTAAGGCCCCGGGTCTTGATGGATTCCCT
GCTCTGTTTTATCAAAAGTATTGGGAGCTGGTGGGCCCGACAACAATCTCCAATTGCCTGTCTATTCTGAATTCAGATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCTATCAGTACTAGCAGACGAAACAGGCAGAATCAGGA
CGAGACGCAGGATCCTACCTAGAGTCGATCTGAAAGGGGATCCAGTACCCCAAGAGGAGGTCGGTAGGCCAGAGAGAGCAGGACCTAGTGATTTGGAAAAGATGTATGGG
ATAGAACGGTTGAAGAAGTTAGGAGCCACAGTGTTTGAGGGTTCCACAGATCCAGCTGACGTCGAGGTCTGGTTAAATATGCTGGAGAAATGCTTCGACGTGATGAGTTG
TCCTGAGGAGCGAAAATTCAGACTAGCTACATTCCTTCTGCAGAAGGAGGCGGAGGGATGGTGGAAATCCATTTTAGCCAGGCGCAGTGGTGCATGTATGTTAGACTGGC
AAATTTTCAGAGGCATATTTGAGGACAAGTACTATCCCACCACATATTGTGAGGCAAAGAGAGATGAGTTTCTGGGGCTAAAGCAAGGGTCACTTTCAGTGGCCGAGTAC
GAGAGAAAGAATATCGAGCTTTCACGGTATGCCGATGTGATTGTGGCATCTGAGAGTGACAGGTGTCGAAGGTTTGAGAAAGGGTTGCGTTTTGAGATACGTACCCCGCG
GAGGTTCGCACCTGGAGTGAATGTTTCAGGTCGTCAAGACTTTAAGCGCCGATATGGTGGCCAGTCATCAAGGCAGATGAGTTCGGGTAGTGCCTATCAGAGGCAGAGTC
AGAGAGCCTCCGTCAGTCTGCGAGTTCAGTAG

mRNA sequence

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Protein sequence

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MWQHEKLGGPDRDMRQISAFREVLDECNLKDLDFSGEMGLHVSNLDWAKSDHRQIELSLEGRMYREVQSRSRRSFKFEEWWTRDNKCGNIIRHAGGWVGARDFTQPLHIA
LKKCAAELVHHLERQLDLLLLEEEIYWKQRSREDWLKWGDRNTKWFHQKASSRRKKIGFEEDVNKVLAYVPRRITADMNLMLTSPFSEEEVLAAVRGFPPSKAPGLDGFP
ALFYQKYWELVGPTTISNCLSILNSDEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSVLADETGRIRTRRRILPRVDLKGDPVPQEEVGRPERAGPSDLEKMYG
IERLKKLGATVFEGSTDPADVEVWLNMLEKCFDVMSCPEERKFRLATFLLQKEAEGWWKSILARRSGACMLDWQIFRGIFEDKYYPTTYCEAKRDEFLGLKQGSLSVAEY
ERKNIELSRYADVIVASESDRCRRFEKGLRFEIRTPRRFAPGVNVSGRQDFKRRYGGQSSRQMSSGSAYQRQSQRASVSLRVQ

Lag0000760 (gene) of Sponge gourd (AG-4) v1 genome