CuGenDBv2

Gene ID	Lag0001200
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	DNA polymerase epsilon subunit
Genome location	chr4:26488571..26489023
RNA-Seq Expression	Lag0001200
Synteny	Lag0001200
Gene Ontology terms	GO:0006261 - DNA-dependent DNA replication (biological process) GO:0006536 - glutamate metabolic process (biological process) GO:0009793 - embryo development ending in seed dormancy (biological process) GO:0042276 - error-prone translesion synthesis (biological process) GO:0051781 - positive regulation of cell division (biological process) GO:0008622 - epsilon DNA polymerase complex (cellular component) GO:0003677 - DNA binding (molecular function) GO:0004351 - glutamate decarboxylase activity (molecular function) GO:0030170 - pyridoxal phosphate binding (molecular function) GO:0070182 - DNA polymerase binding (molecular function)
InterPro domains	IPR016266 - DNA polymerase epsilon, subunit B

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6607399.1 DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia]

1.2e-39

76.52

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Query:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD
        MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI+D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD
Subjt:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD

Query:  VSKFHYDPIKKIFHL
        +SK+ YDPIKKIF++
Subjt:  VSKFHYDPIKKIFHL

KAG6607402.1 DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia]

1.2e-39

76.52

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Query:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD
        MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI+D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD
Subjt:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD

Query:  VSKFHYDPIKKIFHL
        +SK+ YDPIKKIF++
Subjt:  VSKFHYDPIKKIFHL

XP_022949454.1 DNA polymerase epsilon subunit B [Cucurbita moschata]

1.2e-39

76.52

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Query:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD
        MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI+D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD
Subjt:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD

Query:  VSKFHYDPIKKIFHL
        +SK+ YDPIKKIF++
Subjt:  VSKFHYDPIKKIFHL

XP_023525784.1 DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo]

5.3e-40

77.39

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Query:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD
        MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI+D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD
Subjt:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD

Query:  VSKFHYDPIKKIFHL
        +SKF YDPIKKIF++
Subjt:  VSKFHYDPIKKIFHL

XP_038894268.1 DNA polymerase epsilon subunit B [Benincasa hispida]

3.1e-40

78.45

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Query:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF
        MDA TLR+ VQ+K+K RG Y IKL+AL+EIVSF S F GCEDDAIDLVLDNLHDESL+SSILD+DAVHRVVS + +A  VGEESPNTITSTSALCIID+F
Subjt:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF

Query:  DVSKFHYDPIKKIFHL
        D+SKF YDPIKKIF+L
Subjt:  DVSKFHYDPIKKIFHL

TrEMBL top hits

e value

%identity

Alignment

A0A1S4DTQ5 DNA polymerase epsilon subunit

1.6e-34

69.57

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Query:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF
        MDA TLR+ VQ+K+K RG Y IKL+AL+EIVSF S F G EDDAI+LVLD+LH+ESL+S IL++D VHRV+S L +A    E SPNTITST+ALCIID+F
Subjt:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF

Query:  DVSKFHYDPIKKIFH
        D+SKF YDPIKKIFH
Subjt:  DVSKFHYDPIKKIFH

A0A5A7T342 Glutamate decarboxylase

1.6e-34

69.57

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Query:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF
        MDA TLR+ VQ+K+K RG Y IKL+AL+EIVSF S F G EDDAI+LVLD+LH+ESL+S IL++D VHRV+S L +A    E SPNTITST+ALCIID+F
Subjt:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF

Query:  DVSKFHYDPIKKIFH
        D+SKF YDPIKKIFH
Subjt:  DVSKFHYDPIKKIFH

A0A5D3BIF9 Glutamate decarboxylase

1.6e-34

69.57

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Query:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF
        MDA TLR+ VQ+K+K RG Y IKL+AL+EIVSF S F G EDDAI+LVLD+LH+ESL+S IL++D VHRV+S L +A    E SPNTITST+ALCIID+F
Subjt:  MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSF

Query:  DVSKFHYDPIKKIFH
        D+SKF YDPIKKIFH
Subjt:  DVSKFHYDPIKKIFH

A0A6J1GCV3 DNA polymerase epsilon subunit

5.8e-40

76.52

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Query:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD
        MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI+D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD
Subjt:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD

Query:  VSKFHYDPIKKIFHL
        +SK+ YDPIKKIF++
Subjt:  VSKFHYDPIKKIFHL

A0A6J1KGF0 DNA polymerase epsilon subunit

5.8e-40

76.52

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Query:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD
        MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI+D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD
Subjt:  MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFD

Query:  VSKFHYDPIKKIFHL
        +SK+ YDPIKKIF++
Subjt:  VSKFHYDPIKKIFHL

SwissProt top hits

e value

%identity

Alignment

Q500V9 DNA polymerase epsilon subunit B

7.3e-16

47.71

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Query:  RRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCED-DAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFH
        R+ +QKK K RGY++K DAL+EI+ FA  FP  +D +AIDL+LDNL  E+ +SS +D ++V  +++ L  A    EE     TS S+L IID+F V KF 
Subjt:  RRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCED-DAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFH

Query:  YDPIKKIFH
        YD +KK F+
Subjt:  YDPIKKIFH

Arabidopsis top hits

e value

%identity

Alignment

AT5G22110.1 DNA polymerase epsilon subunit B2

5.2e-17

47.71

Show/hide

Query:  RRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCED-DAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFH
        R+ +QKK K RGY++K DAL+EI+ FA  FP  +D +AIDL+LDNL  E+ +SS +D ++V  +++ L  A    EE     TS S+L IID+F V KF 
Subjt:  RRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCED-DAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFH

Query:  YDPIKKIFH
        YD +KK F+
Subjt:  YDPIKKIFH

Sequences

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CDS sequence

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ATGGATGCAACCACTCTAAGGAGAATGGTACAGAAAAAATCAAAATTCAGAGGTTACCACATCAAACTAGATGCTCTCAATGAGATTGTTTCCTTTGCCTCTAGCTTCCC
TGGCTGCGAAGACGATGCTATAGATCTTGTTCTCGACAACCTCCATGACGAATCTTTGCAATCTTCAATATTAGACGAAGACGCAGTGCATCGGGTTGTTAGCAGTTTAC
GCTCAGCTGCTGGAGTGGGAGAAGAAAGTCCTAATACCATTACTAGTACATCTGCTCTTTGCATCATAGACTCTTTTGATGTCTCCAAGTTTCATTACGATCCCATCAAG
AAGATCTTCCACCTGTAA

mRNA sequence

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Protein sequence

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MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIK
KIFHL

Lag0001200 (gene) of Sponge gourd (AG-4) v1 genome