CuGenDBv2

Gene ID	Lag0001480
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	tRNA:m(4)X modification enzyme TRM13
Genome location	chr4:31843507..31846408
RNA-Seq Expression	Lag0001480
Synteny	Lag0001480
Gene Ontology terms	GO:0030488 - tRNA methylation (biological process) GO:0106050 - tRNA 2'-O-methyltransferase activity (molecular function)
InterPro domains	IPR007871 - Methyltransferase TRM13 IPR039044 - tRNA:m(4)X modification enzyme Trm13

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6575728.1 tRNA:m(4)X modification enzyme TRM13, partial [Cucurbita argyrosperma subsp. sororia]

8.3e-67

67.14

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Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISS+E   GL D ISSEMKRNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

KAG7014284.1 tRNA:m(4)X modification enzyme TRM13-like protein [Cucurbita argyrosperma subsp. argyrosperma]

2.2e-67

67.61

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Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISSDE   GL D ISSEMKRNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

XP_022954384.1 tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita moschata]

7.5e-68

68.08

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Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISSDE   GL DSISSEMKRNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

XP_022991622.1 tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita maxima]

9.8e-68

67.61

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Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISSDE   GL DSISSEMKRN++YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

XP_023547814.1 tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita pepo subsp. pepo]

2.2e-67

67.61

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISSDE   G  DSISSEMKRNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

TrEMBL top hits

e value

%identity

Alignment

A0A1S4E343 tRNA:m(4)X modification enzyme TRM13

1.1e-64

65.26

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L GH+KRC LLKQTQSL  Q FYQKGINAGEED +S++ P  GL DSISSEMKRNI+YGM G +FHRLL KI+ALH LICKDIQDSY+IK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGNL+E GVLRNYDGG+Q EGNRSYGN+ANVV A VE GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +VFLVER SYKLK
Subjt:  KVFLVERISYKLK

A0A5A7UYK4 tRNA:m(4)X modification enzyme TRM13

3.8e-65

65.73

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L GH+KRC LLKQTQSL  Q FYQKGINAGEEDL+S++ P  GL DSISSEMKRNI+YGM G +FHRLL KI+ALH LICKDIQDSY+IK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E            K  F+  HVLQQA ILGNL+E GVLRNYDGG+Q EGNRSYGN+ANVV A VE GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +VFLVER SYKLK
Subjt:  KVFLVERISYKLK

A0A5D3CJH7 tRNA:m(4)X modification enzyme TRM13

2.2e-65

65.73

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L GH+KRC LLKQTQSL  Q FYQKGINAGEEDL+S++ P  GL DSISSEMKRNI+YGM G +FHRLL KI+ALH LICKDIQDSY+IK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGNL+E GVLRNYDGG+Q EGNRSYGN+ANVV A VE GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +VFLVER SYKLK
Subjt:  KVFLVERISYKLK

A0A6J1GSU8 tRNA:m(4)X modification enzyme TRM13

3.6e-68

68.08

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISSDE   GL DSISSEMKRNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

A0A6J1JMC6 tRNA:m(4)X modification enzyme TRM13

4.7e-68

67.61

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQTQSL VQ FYQKGINAGEED ISSDE   GL DSISSEMKRN++YGM GAEFHRLL KI+ALHGLICKDIQDSYRIK+     +KG 
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                        E  +         K  F+  HVLQQA ILGN+EE GVLRNYDGGEQCEGNRS GNEANV  A +E GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLK
        +V LVER SYKLK
Subjt:  KVFLVERISYKLK

SwissProt top hits

e value

%identity

Alignment

Q10B19 tRNA:m(4)X modification enzyme TRM13

1.2e-23

36.57

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+ +C L KQ  +L  Q FY KGIN+G        E   G    ++S  KR +++ +   E   L+ KIK  H      ++DS+ +       ++  
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
                       N+  +         K  ++  HV QQA I+GN+E  G+L+   GGE  E      N      A VE GAGRGYLTQMLADCYGI+
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLKVKI
         VFLVER SYKLK+KI
Subjt:  KVFLVERISYKLKVKI

Arabidopsis top hits

e value

%identity

Alignment

AT4G01880.1 methyltransferases

1.5e-42

43.32

Show/hide

Query:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN
        L+ H+KRC LLKQT  L  Q FYQKGINAG ++    +E  +G    ++SEMKRN++Y M  ++FH+L++KI+ +HG ICKDI+DSY   +   C +  N
Subjt:  LDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGN

Query:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK
        + +                          K  F+  HVLQQ  ILGNLEE GVL+  +   +C  +     + N   A VE GAGRGYLTQMLADCYGIK
Subjt:  RYVLVLLGINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIK

Query:  KVFLVERISYKLKV--KIRYFTLTWAEIERVGCSDLLSSPLSMEALH
        KV+LVER SYKLK    +R       E  R+   DL  +  ++E+LH
Subjt:  KVFLVERISYKLKV--KIRYFTLTWAEIERVGCSDLLSSPLSMEALH

Sequences

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CDS sequence

Show/hide CDS sequence

ATGGACTATCTCGATGGGCATCTAAAGAGGTGTCTATTGTTGAAGCAAACTCAATCCTTGGTTGTTCAATCGTTCTATCAAAAGGGTATCAATGCTGGAGAGGAGGATCT
GATATCTTCCGATGAACCAACTTTGGGTTTATTAGATAGCATTTCCTCTGAAATGAAGAGGAATATCATTTATGGCATGTTTGGAGCTGAGTTTCATCGACTTCTTAGAA
AGATTAAAGCTCTTCATGGTCTTATATGCAAGGATATTCAAGACTCTTATAGGATAAAGAAGCTTGCAATATGTGGATTAAAGGGAAATAGATATGTACTTGTGTTGTTA
GGAATTAATTGCTTTCATGGAGTTAATGAGTTTAGTAAAATGTGTTACGTTTTCTTTTGGGTAGGAAAATTCCATTTCAAGAGAACACATGTTCTGCAGCAGGCGTTGAT
TCTTGGAAACTTGGAAGAACCTGGAGTACTAAGGAATTATGATGGAGGGGAACAATGTGAGGGTAACAGATCATATGGCAATGAGGCTAATGTTGTCCTAGCGGAGGTTG
AGGTTGGAGCAGGAAGAGGCTACTTAACTCAAATGCTTGCAGATTGTTATGGTATCAAAAAGGTGTTTCTTGTTGAGAGGATATCATACAAACTGAAGGTTAAAATTAGA
TATTTCACCCTTACATGGGCCGAAATTGAAAGGGTTGGCTGCAGTGACTTGTTGTCATCACCTTTGTCAATGGAAGCATTACACAAACTCATTGACCGTTTTAACCCGAC
ATATGTAAATCACCGCTCCTCAACCTCAAGTGCTCTTGACACTGATAAAGGTTTGAACGTTCTTCCCATGGCATTCTTTGCCATTAATGATGAAGCTCATGTTTCAAGGA
AGTCGTCCCCCAAGTTGACCAACGAGGGCTCTGATGCAAGACTTGATGCTCAACAGAAGAACTTAATCTTCTAA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MDYLDGHLKRCLLLKQTQSLVVQSFYQKGINAGEEDLISSDEPTLGLLDSISSEMKRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKKLAICGLKGNRYVLVLL
GINCFHGVNEFSKMCYVFFWVGKFHFKRTHVLQQALILGNLEEPGVLRNYDGGEQCEGNRSYGNEANVVLAEVEVGAGRGYLTQMLADCYGIKKVFLVERISYKLKVKIR
YFTLTWAEIERVGCSDLLSSPLSMEALHKLIDRFNPTYVNHRSSTSSALDTDKGLNVLPMAFFAINDEAHVSRKSSPKLTNEGSDARLDAQQKNLIF

Lag0001480 (gene) of Sponge gourd (AG-4) v1 genome