CuGenDBv2

Gene ID	Lag0001883
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Non-specific serine/threonine protein kinase
Genome location	chr4:36499980..36505867
RNA-Seq Expression	Lag0001883
Synteny	Lag0001883
Gene Ontology terms	GO:0006521 - regulation of cellular amino acid metabolic process (biological process) GO:0009635 - response to herbicide (biological process) GO:0010998 - regulation of translational initiation by eIF2 alpha phosphorylation (biological process) GO:0018105 - peptidyl-serine phosphorylation (biological process) GO:0005886 - plasma membrane (cellular component) GO:0000049 - tRNA binding (molecular function) GO:0004694 - eukaryotic translation initiation factor 2alpha kinase activity (molecular function) GO:0004712 - protein serine/threonine/tyrosine kinase activity (molecular function) GO:0005515 - protein binding (molecular function) GO:0005524 - ATP binding (molecular function) GO:0106310 - protein serine kinase activity (molecular function)
InterPro domains	IPR006575 - RWD domain IPR016135 - Ubiquitin-conjugating enzyme/RWD-like IPR040213 - GIR2-like

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6592986.1 eIF-2-alpha kinase GCN2, partial [Cucurbita argyrosperma subsp. sororia]

7.2e-55

84.96

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MGHSSKKKRRGGGGGGKR+KGRTP KD+S SGEESEL+S+E+TALC IFQEDCKVV+G  PQVTIKLRPYSNDMGFEDLDVSA LSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPEKGLAKGDTEKLLSLLHEQ +  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

XP_022959907.1 eIF-2-alpha kinase GCN2 isoform X1 [Cucurbita moschata]

7.2e-55

84.96

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MGHSSKKKRRGGGGGGKR+KGRTP KD+S SGEESEL+S+E+TALC IFQEDCKVV+G  PQVTIKLRPYSNDMGFEDLDVSA LSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPEKGLAKGDTEKLLSLLHEQ +  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

XP_023004333.1 eIF-2-alpha kinase GCN2 isoform X1 [Cucurbita maxima]

2.1e-54

84.21

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MGHSSKKKRRGGGGGGKR+KGRTP KD+S SGEE EL+S+E+TALC IFQEDCKVV+G  PQVTIKLRPYSNDMGFEDLDVSA LSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPEKGLAKGDTEKLLSLLHEQ +  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

XP_023514310.1 eIF-2-alpha kinase GCN2 isoform X1 [Cucurbita pepo subsp. pepo]

7.2e-55

84.96

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MGHSSKKKRRGGGGGGKR+KGRTP KD+S SGEESEL+S+E+TALC IFQEDCKVV+G  PQVTIKLRPYSNDMGFEDLDVSA LSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPEKGLAKGDTEKLLSLLHEQ +  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

XP_038885257.1 eIF-2-alpha kinase GCN2 isoform X1 [Benincasa hispida]

1.0e-53

84.21

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MG SSKKKRRGGG GGKR+KGRTPL DYS SGEESEL+++E+TALCAIFQEDCKVVTG  PQVTIKL+PYSNDMGFEDLDVSALLSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPE+GLAKGD EKLLSLLHEQ N  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BCY3 Non-specific serine/threonine protein kinase

1.9e-53

83.46

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MG SSKKKRRGGG GGKR+KGRTPL DYS SGEES+L+++EITALCAIFQEDCKVVTG  PQVTIKL+PYSNDMGFED DVSALLSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPE+GL KGDTEKLLSLLHEQ N  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

A0A1S3BE48 eIF-2-alpha kinase GCN2 isoform X4

1.9e-53

83.46

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MG SSKKKRRGGG GGKR+KGRTPL DYS SGEES+L+++EITALCAIFQEDCKVVTG  PQVTIKL+PYSNDMGFED DVSALLSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPE+GL KGDTEKLLSLLHEQ N  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

A0A6J1D9Z7 Non-specific serine/threonine protein kinase

1.5e-53

85.94

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Query:  KKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKLLITPE
        KKKR GGGG GKR+KGRTPLKDYSLSGEESELLS+E+TA+CAIFQEDCKVVTG PPQVTIKLRPYSN MGFEDLDVSALLSVK+LPGYPYKCPKLLITPE
Subjt:  KKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKLLITPE

Query:  KGLAKGDTEKLLSLLHEQQNEQA-DGEM
        KGL+KGDT+KLLSLLH+Q N  A DG +
Subjt:  KGLAKGDTEKLLSLLHEQQNEQA-DGEM

A0A6J1H5V7 Non-specific serine/threonine protein kinase

3.5e-55

84.96

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MGHSSKKKRRGGGGGGKR+KGRTP KD+S SGEESEL+S+E+TALC IFQEDCKVV+G  PQVTIKLRPYSNDMGFEDLDVSA LSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPEKGLAKGDTEKLLSLLHEQ +  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

A0A6J1KZ64 Non-specific serine/threonine protein kinase

1.0e-54

84.21

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL
        MGHSSKKKRRGGGGGGKR+KGRTP KD+S SGEE EL+S+E+TALC IFQEDCKVV+G  PQVTIKLRPYSNDMGFEDLDVSA LSVK+LPGYPYKCPKL
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKL

Query:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM
        LITPEKGLAKGDTEKLLSLLHEQ +  A DG +
Subjt:  LITPEKGLAKGDTEKLLSLLHEQQNEQA-DGEM

SwissProt top hits

e value

%identity

Alignment

Q9LX30 eIF-2-alpha kinase GCN2

5.9e-36

63.36

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDY-SLSGEESELLSDEITALCAIFQEDCKVVTG--SPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKC
        MG SS KK++  GG G+R +    LKD+ S + E++ELLS+EITAL AIFQEDCKVV+   SPPQ+ IKLRPYS DMG+ED D+SA+L V+ LPGYPYKC
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDY-SLSGEESELLSDEITALCAIFQEDCKVVTG--SPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKC

Query:  PKLLITPEKGLAKGDTEKLLSLLHEQQNEQA
        PKL ITPE+GL   D EKLLSLL +Q N  A
Subjt:  PKLLITPEKGLAKGDTEKLLSLLHEQQNEQA

Arabidopsis top hits

e value

%identity

Alignment

AT3G59410.1 protein kinase family protein

4.2e-37

63.36

Show/hide

Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDY-SLSGEESELLSDEITALCAIFQEDCKVVTG--SPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKC
        MG SS KK++  GG G+R +    LKD+ S + E++ELLS+EITAL AIFQEDCKVV+   SPPQ+ IKLRPYS DMG+ED D+SA+L V+ LPGYPYKC
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDY-SLSGEESELLSDEITALCAIFQEDCKVVTG--SPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKC

Query:  PKLLITPEKGLAKGDTEKLLSLLHEQQNEQA
        PKL ITPE+GL   D EKLLSLL +Q N  A
Subjt:  PKLLITPEKGLAKGDTEKLLSLLHEQQNEQA

AT3G59410.2 protein kinase family protein

4.8e-33

53.55

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Query:  MGHSSKKKRRGGGGGGKRNKGRTPLKDY-SLSGEESELLSDEITAL------------------------CAIFQEDCKVVTG--SPPQVTIKLRPYSND
        MG SS KK++  GG G+R +    LKD+ S + E++ELLS+EITAL                         AIFQEDCKVV+   SPPQ+ IKLRPYS D
Subjt:  MGHSSKKKRRGGGGGGKRNKGRTPLKDY-SLSGEESELLSDEITAL------------------------CAIFQEDCKVVTG--SPPQVTIKLRPYSND

Query:  MGFEDLDVSALLSVKFLPGYPYKCPKLLITPEKGLAKGDTEKLLSLLHEQQNEQA
        MG+ED D+SA+L V+ LPGYPYKCPKL ITPE+GL   D EKLLSLL +Q N  A
Subjt:  MGFEDLDVSALLSVKFLPGYPYKCPKLLITPEKGLAKGDTEKLLSLLHEQQNEQA

AT3G60300.1 RWD domain-containing protein

3.6e-04

22.68

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Query:  ELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKLLITPEKGLAKGDTEKLLSLLHEQQNEQADGEM
        E ++ E+ AL A++ EDC ++   PP + + ++P + ++  +   V A++  +    YP + P++ +   KGL +   + L+ ++ E+ ++ +   M
Subjt:  ELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKLLITPEKGLAKGDTEKLLSLLHEQQNEQADGEM

Sequences

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CDS sequence

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ATGGGGCATAGTTCCAAGAAGAAGCGGCGTGGTGGGGGAGGGGGTGGGAAGAGGAACAAGGGAAGGACGCCACTGAAAGATTACTCGTTGAGTGGTGAAGAGAGTGAGCT
TCTATCAGACGAGATTACTGCCTTGTGTGCGATATTTCAAGAAGACTGCAAAGTTGTCACTGGCTCACCTCCTCAAGTTACCATCAAACTTAGGCCATATTCAAATGACA
TGGGGTTTGAAGATCTAGATGTATCTGCTCTTCTTTCAGTGAAGTTTTTGCCTGGATACCCATACAAATGCCCAAAGTTGCTTATTACCCCAGAGAAAGGTTTGGCAAAA
GGTGACACTGAAAAGTTGCTTTCTCTTCTTCATGAACAGCAAAACGAACAAGCCGATGGGGAAATGCAACGCGAGGAGGTTTACACTGCATAA

mRNA sequence

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Protein sequence

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MGHSSKKKRRGGGGGGKRNKGRTPLKDYSLSGEESELLSDEITALCAIFQEDCKVVTGSPPQVTIKLRPYSNDMGFEDLDVSALLSVKFLPGYPYKCPKLLITPEKGLAK
GDTEKLLSLLHEQQNEQADGEMQREEVYTA

Lag0001883 (gene) of Sponge gourd (AG-4) v1 genome