CuGenDBv2

Gene ID	Lag0007981
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	RNase H domain-containing protein
Genome location	chr9:9304276..9305483
RNA-Seq Expression	Lag0007981
Synteny	Lag0007981
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

CAB4274760.1 unnamed protein product [Prunus armeniaca]

5.9e-12

37.58

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W   + G  K+N DA+W+A R  GGVGW +RDS G  + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +A +A+
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

CAB4274761.1 unnamed protein product [Prunus armeniaca]

5.9e-12

37.58

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W   + G  K+N DA+W+A R  GGVGW +RDS G  + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +A +A+
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

CAB4303756.1 unnamed protein product [Prunus armeniaca]

2.2e-11

36.91

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W     G  K+N DA+W+A R  GGVGW +RDS G  + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +A + +
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

XP_022155262.1 uncharacterized protein LOC111022403 [Momordica charantia]

1.4e-13

37.76

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Query:  WNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRIL-PIIVESDALCVVYLLNGEDE
        W P     W LN DASW+    RGG+GW +R  +G  + AG   +    ++K+LE  AI+EGLR L        NL +L P+ +E+D+  V  LLN + E
Subjt:  WNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRIL-PIIVESDALCVVYLLNGEDE

Query:  DLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        DL++  ++VE+IL ++   E ++F    R  N  AH LA+ A+
Subjt:  DLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

XP_025877173.1 uncharacterized protein LOC107278050 isoform X1 [Oryza sativa Japonica Group]

1.5e-10

36.17

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Query:  WNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYLLNGEDED
        W     G  KLNVD S++A R +GG+G  +RD+ GS ++A    +    +    E+RA +EGL     I A +  +R  PI++E+D + +V LL   + D
Subjt:  WNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYLLNGEDED

Query:  LSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYA
        LSE + LV++I ++  E   V      RS+N  +HLLA  A
Subjt:  LSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYA

TrEMBL top hits

e value

%identity

Alignment

A0A6J1DNV9 uncharacterized protein LOC111022403

6.8e-14

37.76

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Query:  WNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRIL-PIIVESDALCVVYLLNGEDE
        W P     W LN DASW+    RGG+GW +R  +G  + AG   +    ++K+LE  AI+EGLR L        NL +L P+ +E+D+  V  LLN + E
Subjt:  WNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRIL-PIIVESDALCVVYLLNGEDE

Query:  DLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        DL++  ++VE+IL ++   E ++F    R  N  AH LA+ A+
Subjt:  DLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

A0A6J5UE59 Reverse transcriptase domain-containing protein

2.9e-12

37.58

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W   + G  K+N DA+W+A R  GGVGW +RDS G  + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +A +A+
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

A0A6J5UF50 Uncharacterized protein

2.9e-12

37.58

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W   + G  K+N DA+W+A R  GGVGW +RDS G  + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +A +A+
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

A0A6J5WPU6 Reverse transcriptase domain-containing protein

1.1e-11

36.91

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W     G  K+N DA+W+A R  GGVGW +RDS G  + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +A + +
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

A0A6J5YDN0 Uncharacterized protein

1.2e-10

35.57

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Query:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL
        S P+   W     G  K+N DA+W+A R  GGVGW +RDS    + AG        S  M+EL AI   L       +   N  +  IIVESD+   + +
Subjt:  SQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDALCVVYL

Query:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA
        LNG     S+   +V DI ++      VSFVF PRS N+ AH +  + +
Subjt:  LNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAA

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.3e-09

29.32

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Query:  VKRLILKHSKHSNLDNLEGRSASEKKSQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILP
        ++R +    + S    LEG+++  +  +     W        K N DA+W     R G+GW +R+  G  ++ G   + ++ ++    L A +E LR   
Subjt:  VKRLILKHSKHSNLDNLEGRSASEKKSQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILP

Query:  AIRASRPNLRILPIIVESDALCVVYLLNGED--EDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYA-AFGEFCSPRFSFGP
         +  SR N +   II ESDA  +V LLN +D    L  A   +EDI ++    E V F F PR  N+ A  +AR + +F  +    FS  P
Subjt:  AIRASRPNLRILPIIVESDALCVVYLLNGED--EDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYA-AFGEFCSPRFSFGP

AT4G29090.1 Ribonuclease H-like superfamily protein

4.2e-08

27.62

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Query:  EGRSASEKKSQPSHALWNPSASGEW--------KLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNL
        E R  +E +S  +    N S+ G W        K N DA+WN    R G+GW +R+ +G   + G   + K  S+   EL A+   +  L   + +    
Subjt:  EGRSASEKKSQPSHALWNPSASGEW--------KLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNL

Query:  RILPIIVESDALCVVYLLNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYA-AFGEFCSPRFSFGPS
            +I ESD+  ++ +LN  DE        ++D+ ++  +   V FVF PR  N  A  +AR + +F  +    +S  PS
Subjt:  RILPIIVESDALCVVYLLNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYA-AFGEFCSPRFSFGPS

Sequences

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CDS sequence

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ATGACATTATCTGGAATTGTGACCCCAAAGACCATTTCTCTGTTAGGAGCGTTTATCATCTGGCATCGCATCTCATTAACTCCTAGAAGCCGTCATCGTCTTCAGGAGAA
GAAGCTAGTCCCTTGTGGAGAAATTTTTGGAAGATTAAAGCCATTCCAAAGGCAAAAATCTGTGCGTGGAGGATTATTCAGAACTCCCTCCCTACCTATCAAAATATTAG
GAAGAAAGGAATTCAGGCTGATCCCCTTTGCAGCCGTTGCAGAAAGCACGAAGAATCTGTGGTGCACGCAATTTGGGAGTGTAAAAAGATTAATTCTAAAGCATTCAAAG
CATTCAAACCTGGATAATTTGGAGGGTAGATCCGCCAGTGAGAAGAAGAGTCAGCCGAGTCATGCTCTGTGGAATCCGTCAGCTTCGGGAGAGTGGAAATTGAATGTTGA
CGCCTCTTGGAATGCCCTCCGATCTCGAGGAGGGGTGGGTTGGACCATGCGTGACTCGGAAGGATCTCCAATCTACGCGGGTGTATGTCGAATTTTCAAATCGTGGTCGA
TCAAGATGTTGGAGCTGAGGGCGATTGTGGAAGGTCTCCGAATCTTGCCGGCGATTAGAGCCTCAAGACCGAACCTGCGGATCCTACCGATTATAGTGGAATCAGACGCC
TTGTGCGTGGTTTACCTTCTCAATGGAGAAGACGAGGACCTCTCGGAAGCTTCGTTTCTGGTTGAGGATATTCTCAAAATGAAGATGGAAGCTGAAGCAGTCTCCTTTGT
TTTTTGTCCTAGATCGCGCAATGAGGAGGCTCATCTCTTGGCGCGTTATGCAGCTTTTGGGGAGTTCTGTTCCCCACGTTTTTCTTTTGGTCCCTCTTCCATTTCGGAAG
AGGACTTTTTCTGTTTTATTGGGCTTGGGCCCAGTGACTTCTCTTCCTTGTTGGGAGCTGGTTTTGCTGGTGTTGGTGTGTTTTAA

mRNA sequence

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Protein sequence

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MTLSGIVTPKTISLLGAFIIWHRISLTPRSRHRLQEKKLVPCGEIFGRLKPFQRQKSVRGGLFRTPSLPIKILGRKEFRLIPFAAVAESTKNLWCTQFGSVKRLILKHSK
HSNLDNLEGRSASEKKSQPSHALWNPSASGEWKLNVDASWNALRSRGGVGWTMRDSEGSPIYAGVCRIFKSWSIKMLELRAIVEGLRILPAIRASRPNLRILPIIVESDA
LCVVYLLNGEDEDLSEASFLVEDILKMKMEAEAVSFVFCPRSRNEEAHLLARYAAFGEFCSPRFSFGPSSISEEDFFCFIGLGPSDFSSLLGAGFAGVGVF

Lag0007981 (gene) of Sponge gourd (AG-4) v1 genome