CuGenDBv2

Gene ID	Lag0010523
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr1:544071..544463
RNA-Seq Expression	Lag0010523
Synteny	Lag0010523
Gene Ontology terms	GO:0090304 - nucleic acid metabolic process (biological process) GO:0016020 - membrane (cellular component) GO:0140640 - catalytic activity, acting on a nucleic acid (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAA3467389.1 reverse transcriptase [Gossypium australe]

1.4e-28

56.14

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S GGKE+ + AI+Q I  YTM+CF L  S C E+  + ++FWW  S  KR   W +WK LS SKE GGMG RD+K FN +LLAK  WR+LRNPNSLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
         RTL+ KY+KD  F
Subjt:  ARTLRGKYFKDETF

XP_022145148.1 uncharacterized protein LOC111014662 [Momordica charantia]

1.7e-29

55.26

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S GGKE+L+ A+ QAI NY+M+CF    ++C+EIN + A FWWGS+  ++K  W SWK L  SKE GG+G +D+ +FNQ +LAK SW+I++NPNSLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
         R LRGKYFK   F
Subjt:  ARTLRGKYFKDETF

XP_022150918.1 uncharacterized protein LOC111018954 [Momordica charantia]

2.0e-27

53.91

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Query:  AKKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSL
        AK  S+GGKE+L+ A+ QAI  YTM+CF L   +  E + + A FWWGSSKE +K  W++W +L   K  GGMG RD++LFN+ LLAK  WRIL +PNS+
Subjt:  AKKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSL

Query:  LARTLRGKYFKDETF
        L+R L+G+YFKD +F
Subjt:  LARTLRGKYFKDETF

XP_022155286.1 uncharacterized protein LOC111022423 [Momordica charantia]

2.3e-31

59.65

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S+GGKE+L+  + QAI NYT++CF L  S+C E++++   FWWGSS E RK  W SWK L   K+LGGMG RDI +FNQ +LAK SWRILR+P+SLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
        A+TLRGKYFK  +F
Subjt:  ARTLRGKYFKDETF

XP_030940187.1 uncharacterized protein LOC115965136 [Quercus lobata]

1.3e-26

51.35

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Query:  SLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLLART
        S  G+EIL+  + QA   YTM+CF L +++C+E+N M + FWWG   ++RK  W++W+ L TSKE GGMG RD+K FN  LLAK  WRI +NPN+L+ + 
Subjt:  SLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLLART

Query:  LRGKYFKDETF
        L+ KYF D TF
Subjt:  LRGKYFKDETF

TrEMBL top hits

e value

%identity

Alignment

A0A5B6VE25 Reverse transcriptase

6.8e-29

56.14

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S GGKE+ + AI+Q I  YTM+CF L  S C E+  + ++FWW  S  KR   W +WK LS SKE GGMG RD+K FN +LLAK  WR+LRNPNSLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
         RTL+ KY+KD  F
Subjt:  ARTLRGKYFKDETF

A0A6J1CV63 uncharacterized protein LOC111014662

8.0e-30

55.26

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S GGKE+L+ A+ QAI NY+M+CF    ++C+EIN + A FWWGS+  ++K  W SWK L  SKE GG+G +D+ +FNQ +LAK SW+I++NPNSLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
         R LRGKYFK   F
Subjt:  ARTLRGKYFKDETF

A0A6J1DAR4 uncharacterized protein LOC111018954

9.8e-28

53.91

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Query:  AKKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSL
        AK  S+GGKE+L+ A+ QAI  YTM+CF L   +  E + + A FWWGSSKE +K  W++W +L   K  GGMG RD++LFN+ LLAK  WRIL +PNS+
Subjt:  AKKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSL

Query:  LARTLRGKYFKDETF
        L+R L+G+YFKD +F
Subjt:  LARTLRGKYFKDETF

A0A6J1DRA0 uncharacterized protein LOC111022423

1.1e-31

59.65

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S+GGKE+L+  + QAI NYT++CF L  S+C E++++   FWWGSS E RK  W SWK L   K+LGGMG RDI +FNQ +LAK SWRILR+P+SLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
        A+TLRGKYFK  +F
Subjt:  ARTLRGKYFKDETF

A0A803PKJ2 Uncharacterized protein

2.8e-27

52.63

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        KK S+GG E+LL A+VQAI  Y M+CF LS   C++I  M A FWWGSS + +K  W SWK+L TSK  GG+G R    FNQ  LAK +WR+ +NP+SLL
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDETF
        +R L+G+Y+    F
Subjt:  ARTLRGKYFKDETF

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

1.2e-11

31.25

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Query:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL
        K  S  G+  L  A++ ++  ++M+   L  S+   ++++   F WGS+ EK+K   + W  + + K+ GG+G R  K  N+ L++K+ WR+L+  NSL 
Subjt:  KKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLL

Query:  ARTLRGKYFKDE
           L+ KY   E
Subjt:  ARTLRGKYFKDE

P93295 Uncharacterized mitochondrial protein AtMg00310

2.3e-18

48.31

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Query:  YTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKE-LGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTLRGKYF
        Y M+CF LS  +C+++      FWW S + KRK  W++W+ L  SKE  GG+G RD+  FNQ LLAK S+RI+  P++LL+R LR +YF
Subjt:  YTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKE-LGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTLRGKYF

Arabidopsis top hits

e value

%identity

Alignment

AT4G29090.1 Ribonuclease H-like superfamily protein

3.4e-17

41.3

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Query:  AILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTLRGKYF
        A+  YTM CF L  ++C++I  + A+FWW + +E +   W +W +LS  K  GG+G +DI+ FN  LL K  WR+L  P SL+A+  + +YF
Subjt:  AILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTLRGKYF

ATMG00310.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

1.7e-19

48.31

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Query:  YTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKE-LGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTLRGKYF
        Y M+CF LS  +C+++      FWW S + KRK  W++W+ L  SKE  GG+G RD+  FNQ LLAK S+RI+  P++LL+R LR +YF
Subjt:  YTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKE-LGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTLRGKYF

Sequences

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CDS sequence

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ATGCTCTCCAAAATTGCAAAGAAAAGCTCTTTGGGAGGGAAAGAAATTCTCTTAAATGCCATTGTTCAAGCTATACTTAATTACACAATGAATTGTTTTGGACTTTCCAA
TTCGATGTGTGAGGAGATAAATAGAATGCGTGCAAACTTTTGGTGGGGCTCATCTAAAGAGAAGAGAAAGGCACCCTGGATGAGTTGGAAAAATCTCAGCACAAGCAAAG
AATTAGGAGGTATGGGCTCTAGAGATATCAAGTTGTTCAATCAAACTTTGTTAGCCAAGATTAGTTGGAGAATTCTAAGAAATCCGAATAGCCTCCTTGCAAGAACGCTT
CGTGGGAAATATTTCAAGGACGAAACTTTCTCAATGCTCCCTTGGGAAACAACCCCTCCCTAA

mRNA sequence

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Protein sequence

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MLSKIAKKSSLGGKEILLNAIVQAILNYTMNCFGLSNSMCEEINRMRANFWWGSSKEKRKAPWMSWKNLSTSKELGGMGSRDIKLFNQTLLAKISWRILRNPNSLLARTL
RGKYFKDETFSMLPWETTPP

Lag0010523 (gene) of Sponge gourd (AG-4) v1 genome