CuGenDBv2

Gene ID	Lag0012954
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr1:46083470..46084372
RNA-Seq Expression	Lag0012954
Synteny	Lag0012954
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

GAU33827.1 hypothetical protein TSUD_221650 [Trifolium subterraneum]

2.7e-18

32.16

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Query:  NCPVCQEELETTDHALFLCKRAREGLD-IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDY-ISEYRTTNPKGGA---LVQSEKDVTRIIS
        NCPVC EE+E   H  F    + E  D + R+ +  W IW++RN   W  N  +P   GR     W D Y + + ++ N  G     LV+ EK       
Subjt:  NCPVCQEELETTDHALFLCKRAREGLD-IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDY-ISEYRTTNPKGGA---LVQSEKDVTRIIS

Query:  MSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMW
          + +  N DVA++   G++ +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L+++I+ +  +G+S   S + 
Subjt:  MSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMW

Query:  DIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        DI  + S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  DIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

GAU36566.1 hypothetical protein TSUD_277720 [Trifolium subterraneum]

6.7e-17

29.58

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Query:  NCPVCQEELETTDHALFLCKRARE-----GLD----------------------------IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWID
        NCPVC EE+E   H  F C  AR+     GL                             + R+ +  WSIW++RN   W  N  +P  TGR     W D
Subjt:  NCPVCQEELETTDHALFLCKRARE-----GLD----------------------------IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWID

Query:  DYISEYRTTNPKGGALVQSEKDVTRIISMS-EEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRL
         Y      +N        +E+D+ R    + + +  N D A++   G++ +GL  RD SG  + A          S++  EA A+L  +  AR  G+ R+
Subjt:  DYISEYRTTNPKGGALVQSEKDVTRIISMS-EEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRL

Query:  TVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
           SDS  L+++I+ +  +G+S   S + DI  + S+F     NFV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  TVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

GAU40431.1 hypothetical protein TSUD_397500 [Trifolium subterraneum]

3.6e-18

31.89

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Query:  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYISEYRTTNPKGGA----LVQSEKDVTRIISM
        NCPVC EE+E   H  F  K +     + R+ +  W IW++RN   W  N  +P+  GR     W D Y      +N   G     LV+ EK     +  
Subjt:  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYISEYRTTNPKGGA----LVQSEKDVTRIISM

Query:  SEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWD
              N DVA++   G++ +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L+  I+ +  +G+S   S + D
Subjt:  SEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWD

Query:  IHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        I D  S+F      FV R+ N +AH+LA A          +N P  IE+L   E
Subjt:  IHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

GAU41000.1 hypothetical protein TSUD_92250 [Trifolium subterraneum]

8.8e-17

30.82

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Query:  NGNCPVCQEELETTDHALFLCKRARE-----GLD----------------------------IERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EW
        N NCPVC EE+E   H  F C  AR+     GL                             + R+ +  W IW +RN   WI N   P   GR     W
Subjt:  NGNCPVCQEELETTDHALFLCKRARE-----GLD----------------------------IERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EW

Query:  IDDYI-------SEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLA
         D Y        SE RTT      LV+ EK     +        N DVA++   G++ +GL  R+  GQ++         V +S  G EA A+L  +  A
Subjt:  IDDYI-------SEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLA

Query:  RSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        R  G+ R+   SDS  LV++I+ +  +G+S   S + DI    S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  RSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

GAU47930.1 hypothetical protein TSUD_14000 [Trifolium subterraneum]

6.7e-17

33.08

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Query:  NGNCPVCQEELETTDHALFLCKRAREGLDI-ERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYI-------SEYRTTNPKGGALVQSEKDV
        N NCPVC EE++   H  F    + E  DI  R+ +  W IW +RN   W  N   P   GR     W D Y        SE RTT      LV+ EK  
Subjt:  NGNCPVCQEELETTDHALFLCKRAREGLDI-ERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYI-------SEYRTTNPKGGALVQSEKDV

Query:  TRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCI
           +        N DVA++   G++ +GL  R+  GQ++         V +S  G EA A+L  +  AR  G+ R+   SDS  LV++I+ +  +G+S  
Subjt:  TRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCI

Query:  TSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
         S + DI    S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  TSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

TrEMBL top hits

e value

%identity

Alignment

A0A2Z6N9N6 ZnF_PMZ domain-containing protein

1.7e-18

31.89

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Query:  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYISEYRTTNPKGGA----LVQSEKDVTRIISM
        NCPVC EE+E   H  F  K +     + R+ +  W IW++RN   W  N  +P+  GR     W D Y      +N   G     LV+ EK     +  
Subjt:  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYISEYRTTNPKGGA----LVQSEKDVTRIISM

Query:  SEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWD
              N DVA++   G++ +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L+  I+ +  +G+S   S + D
Subjt:  SEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWD

Query:  IHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        I D  S+F      FV R+ N +AH+LA A          +N P  IE+L   E
Subjt:  IHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

A0A2Z6NUW6 CCHC-type domain-containing protein

1.3e-18

32.16

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Query:  NCPVCQEELETTDHALFLCKRAREGLD-IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDY-ISEYRTTNPKGGA---LVQSEKDVTRIIS
        NCPVC EE+E   H  F    + E  D + R+ +  W IW++RN   W  N  +P   GR     W D Y + + ++ N  G     LV+ EK       
Subjt:  NCPVCQEELETTDHALFLCKRAREGLD-IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDY-ISEYRTTNPKGGA---LVQSEKDVTRIIS

Query:  MSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMW
          + +  N DVA++   G++ +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L+++I+ +  +G+S   S + 
Subjt:  MSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMW

Query:  DIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        DI  + S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  DIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

A0A2Z6NW00 Uncharacterized protein

4.3e-17

30.82

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Query:  NGNCPVCQEELETTDHALFLCKRARE-----GLD----------------------------IERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EW
        N NCPVC EE+E   H  F C  AR+     GL                             + R+ +  W IW +RN   WI N   P   GR     W
Subjt:  NGNCPVCQEELETTDHALFLCKRARE-----GLD----------------------------IERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EW

Query:  IDDYI-------SEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLA
         D Y        SE RTT      LV+ EK     +        N DVA++   G++ +GL  R+  GQ++         V +S  G EA A+L  +  A
Subjt:  IDDYI-------SEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLA

Query:  RSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        R  G+ R+   SDS  LV++I+ +  +G+S   S + DI    S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  RSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

A0A2Z6P8I4 Uncharacterized protein

7.3e-17

31.08

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Query:  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMS-EE
        NCPVC EE+E   H +F          + R+ +  W IW +RN   W  N  +P   GR     W D Y      +N   G    +E DV R    + + 
Subjt:  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMS-EE

Query:  IILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHD
        +  N DVA++   G++ +GL  RD SG  + A          S++  EA A+L  +  AR  G++R+   SD   L+++I+ +  +G+S   S + DI  
Subjt:  IILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHD

Query:  IRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
        + S+F      FV R+ N +AH+LA A     S    +N P  IE L   E
Subjt:  IRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

A0A2Z6PCM8 Uncharacterized protein

3.3e-17

33.08

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Query:  NGNCPVCQEELETTDHALFLCKRAREGLDI-ERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYI-------SEYRTTNPKGGALVQSEKDV
        N NCPVC EE++   H  F    + E  DI  R+ +  W IW +RN   W  N   P   GR     W D Y        SE RTT      LV+ EK  
Subjt:  NGNCPVCQEELETTDHALFLCKRAREGLDI-ERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYI-------SEYRTTNPKGGALVQSEKDV

Query:  TRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCI
           +        N DVA++   G++ +GL  R+  GQ++         V +S  G EA A+L  +  AR  G+ R+   SDS  LV++I+ +  +G+S  
Subjt:  TRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCI

Query:  TSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE
         S + DI    S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Subjt:  TSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT3G09510.1 Ribonuclease H-like superfamily protein

6.3e-05

22.82

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Query:  VNGNCPVCQEELETTDHALFLCKRA---------------------REGL-------------DIERICVA--AWSIWNDRNTWIHN--HPIPTAT----
        ++ +CP C  E E+ +HALF C  A                      E +             D  ++      W IW  RN  + N     P+ T    
Subjt:  VNGNCPVCQEELETTDHALFLCKRA---------------------REGL-------------DIERICVA--AWSIWNDRNTWIHN--HPIPTAT----

Query:  --GRCEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGI---------GLIMRDRSGQIIQAAQAMISPVSTSALGAEAE
             +W++   S  +T +P            TR I+ ++    N    Y+     +G          G I+R+  G  I      ++  S + L AE +
Subjt:  --GRCEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGI---------GLIMRDRSGQIIQAAQAMISPVSTSALGAEAE

Query:  AVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAG-----LYSPS---VLWLDNY
        A+L  L+     G +++ +  D   L+  IN       S + + + DI    + F  + F F+ R+ N +AH LA  G      YS S    +WLD Y
Subjt:  AVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAG-----LYSPS---VLWLDNY

AT4G09490.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.3e-05

29.2

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Query:  EEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDI
        + + + TD A+       G G ++R+         Q+    V    L AEA A+   L+ A+S+GI++L++ SDS  L+ +I  E    +      ++DI
Subjt:  EEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDI

Query:  HDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVL
         ++   F  V+F+FV R  N +A  LA + L S S++
Subjt:  HDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVL

Sequences

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CDS sequence

Show/hide CDS sequence

ATGAATAATCTCAGGTATCACCATGTTCTTGTTAACGGAAATTGTCCTGTTTGTCAGGAAGAGCTGGAGACCACAGATCATGCTCTATTCCTTTGCAAGAGAGCCAGGGA
GGGGTTGGATATTGAAAGGATTTGTGTCGCAGCATGGTCCATTTGGAACGATAGGAATACTTGGATCCATAACCATCCCATTCCTACGGCTACAGGGAGATGTGAATGGA
TTGATGATTATATATCCGAATATAGGACGACTAATCCCAAGGGTGGGGCTCTTGTACAGTCAGAGAAGGATGTTACCAGGATTATCTCAATGAGTGAAGAGATTATTTTA
AATACCGATGTAGCGTATATGGGAGCTATTGGTAAAAGTGGAATTGGGTTAATCATGCGGGATAGATCGGGACAAATAATCCAGGCGGCTCAGGCAATGATCTCTCCAGT
TTCTACTTCAGCGTTAGGGGCGGAAGCGGAGGCAGTGTTACAAGGGCTTCGACTAGCCAGATCAATGGGCATTAGTCGGTTAACAGTTATGTCTGATTCTCTGAATCTGG
TTAAATCGATTAATAAGGAGATTAATCAAGGCGATTCGTGCATTACTTCCACTATGTGGGATATTCATGATATACGATCAAACTTCACCAAGGTGACCTTTAACTTTGTT
AGTAGGAGATTCAACGACATTGCCCACTCATTGGCAAATGCAGGTCTTTATTCACCTTCCGTATTATGGTTGGATAACTATCCGGAATGGATAGAAAATTTGTCGTCACA
AGAACGACGTCTGTTTGTATCCCTTCAGGGGCATTCTGATTATTAA

mRNA sequence

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Protein sequence

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MNNLRYHHVLVNGNCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRNTWIHNHPIPTATGRCEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMSEEIIL
NTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFV
SRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQERRLFVSLQGHSDY

Lag0012954 (gene) of Sponge gourd (AG-4) v1 genome