CuGenDBv2

Gene ID	Lag0013613
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	DNA-directed RNA polymerases IV and V subunit 4-like
Genome location	chr1:51592354..51594283
RNA-Seq Expression	Lag0013613
Synteny	Lag0013613
Gene Ontology terms	GO:0006352 - DNA-templated transcription, initiation (biological process) GO:0005634 - nucleus (cellular component) GO:0030880 - RNA polymerase complex (cellular component) GO:0000166 - nucleotide binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0005509 - calcium ion binding (molecular function)
InterPro domains	IPR010997 - HRDC-like superfamily IPR038324 - Rpb4/RPC9 superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6594716.1 DNA-directed RNA polymerases IV and V subunit 4, partial [Cucurbita argyrosperma subsp. sororia]

1.9e-78

95.73

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSK KKGRKVQFDAQGSVDAQIN SLK+SGKNGDLGKGGKG+NG KASV+KE QPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

KAG7026685.1 DNA-directed RNA polymerases IV and V subunit 4, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.2e-77

92.9

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKD---ASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKSSASKD   +SLKGKDDSLSK KKGRKVQFDAQGSVDAQIN SLK+SGKNGDLGKGGKG+NG KASV+KE QPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKD---ASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP
        QELPKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP

XP_022926857.1 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1 [Cucurbita moschata]

2.8e-77

94.51

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSK KKGRKVQFDAQGSVDAQIN SLK+SGKNGDLGKGGKG+NG KASV+KE QPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTE VRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

XP_023003382.1 DNA-directed RNA polymerases IV and V subunit 4-like [Cucurbita maxima]

1.8e-76

93.29

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKS ASKDASLKGKDDSLSK KKGRKVQFDAQGSVDA IN S+K+ GKNGDLGKGGKG+NG KASV+KE QPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

XP_023517145.1 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1 [Cucurbita pepo subsp. pepo]

1.2e-77

95.12

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSK KKGRKVQFDAQGSVDAQIN SLK+SGKNGDLGKGGKG+NG KASV+KE QPLELKIE EL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

TrEMBL top hits

e value

%identity

Alignment

A0A5A7SHG0 Calcineurin B-like protein 3

2.0e-73

89.41

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGF VQKKP KSSLKSSA KDASLKGKDDSLSK KKGRKVQFDAQGSVDA  +FS+KYSGKNGD+GKGGKG N  KASV KEPQ LELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYPWFYE
        PKNVKCQCLMDCEAAQLLQGIQDQMV LSADPTIKIPTSFDRGLQYAKRANHYVN ESVRPVLEYP F E
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYPWFYE

A0A6J1EGC4 DNA-directed RNA polymerases IV and V subunit 4-like isoform X2

5.3e-74

92.07

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKSSASKD    GKDDSLSK KKGRKVQFDAQGSVDAQIN SLK+SGKNGDLGKGGKG+NG KASV+KE QPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTE VRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

A0A6J1EJD9 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1

1.3e-77

94.51

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSK KKGRKVQFDAQGSVDAQIN SLK+SGKNGDLGKGGKG+NG KASV+KE QPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTE VRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

A0A6J1IMM9 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1

6.5e-72

89.63

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKG P+ KKPGKSSLKSS+ KDASLKGKDDSL KPKKGRKVQFDAQGSVDAQINFS+KYSGKNG+LGKGGKG +GA     KEPQPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQ+LQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVL+
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

A0A6J1KMD1 DNA-directed RNA polymerases IV and V subunit 4-like

8.7e-77

93.29

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL
        MSEKGEKGFPVQKKPGKSSLKS ASKDASLKGKDDSLSK KKGRKVQFDAQGSVDA IN S+K+ GKNGDLGKGGKG+NG KASV+KE QPLELKIEQEL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQEL

Query:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  PKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

SwissProt top hits

e value

%identity

Alignment

O48890 DNA-directed RNA polymerase II subunit 4

9.2e-07

37.18

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Query:  KEPQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        +E    ELKI  E    +K +CLM+CE + +L+   +Q+  +S DP  ++   F++ LQY KR + Y N ++VR V E
Subjt:  KEPQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

Q6DBA5 DNA-directed RNA polymerases IV and V subunit 4

1.0e-18

45.51

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQ---INFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIE
        MSEKG KG        KSSLK   SKD    GKD S +K KKGRK+ FD Q +  A    +N S           K GK     K+S        ELK  
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQ---INFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
         +LP+N   +C+MDCEA Q+L GI+ Q+V LS DP+IKIP S+DR L Y +   HY N +SVR VLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

Arabidopsis top hits

e value

%identity

Alignment

AT4G15950.1 RNA polymerase II, Rpb4, core protein

7.4e-20

45.51

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQ---INFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIE
        MSEKG KG        KSSLK   SKD    GKD S +K KKGRK+ FD Q +  A    +N S           K GK     K+S        ELK  
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQ---INFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
         +LP+N   +C+MDCEA Q+L GI+ Q+V LS DP+IKIP S+DR L Y +   HY N +SVR VLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

AT5G09920.1 RNA polymerase II, Rpb4, core protein

6.5e-08

37.18

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Query:  KEPQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        +E    ELKI  E    +K +CLM+CE + +L+   +Q+  +S DP  ++   F++ LQY KR + Y N ++VR V E
Subjt:  KEPQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

Sequences

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CDS sequence

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ATGTCGGAGAAGGGCGAAAAGGGTTTTCCTGTGCAGAAGAAACCTGGAAAGTCTTCGCTCAAATCTTCGGCTTCCAAGGATGCTTCTCTAAAAGGAAAGGATGATAGTTT
ATCAAAGCCGAAGAAGGGAAGGAAAGTCCAGTTTGATGCTCAAGGATCAGTTGATGCGCAGATCAATTTTTCATTGAAATACAGTGGCAAAAATGGTGATTTGGGTAAAG
GAGGAAAAGGTGTAAATGGTGCGAAGGCTTCTGTTACAAAGGAACCACAACCGCTAGAATTGAAGATTGAGCAAGAACTTCCCAAGAATGTTAAATGCCAATGCCTTATG
GACTGTGAGGCTGCACAACTTTTACAGGGAATCCAAGATCAGATGGTTCTTCTATCAGCAGATCCAACCATTAAAATCCCTACGTCATTTGATAGGGGACTGCAATATGC
TAAAAGAGCCAACCACTACGTTAATACCGAGTCAGTCAGACCAGTTCTTGAGTATCCTTGGTTTTATGAATAG

mRNA sequence

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Protein sequence

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MSEKGEKGFPVQKKPGKSSLKSSASKDASLKGKDDSLSKPKKGRKVQFDAQGSVDAQINFSLKYSGKNGDLGKGGKGVNGAKASVTKEPQPLELKIEQELPKNVKCQCLM
DCEAAQLLQGIQDQMVLLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYPWFYE

Lag0013613 (gene) of Sponge gourd (AG-4) v1 genome