CuGenDBv2

Gene ID	Lag0013731
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr1:52322693..52323067
RNA-Seq Expression	Lag0013731
Synteny	Lag0013731
Gene Ontology terms	GO:0090304 - nucleic acid metabolic process (biological process) GO:0016020 - membrane (cellular component) GO:0005488 - binding (molecular function) GO:0140640 - catalytic activity, acting on a nucleic acid (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

XP_023881891.1 uncharacterized protein LOC111994244 [Quercus suber]

1.1e-25

56.31

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        M+KM F E W+K VM CI+SVSYSIL+N        PTRG+RQGDP+SPY+FLL A+GFS+LLN       +SG  I + CP +THLFFADDS++F K+ 
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQK
         Q+
Subjt:  QQK

XP_023902041.1 uncharacterized protein LOC112013897 [Quercus suber]

1.8e-25

55.34

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        M+KM F E W+K VM CI+SVSYSIL+N        PTRG+RQGDP+SPY+FLL A+GFS+LLN       +SG  I + CP +THLFFADD+++F K+ 
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQK
         Q+
Subjt:  QQK

XP_023923071.1 uncharacterized protein LOC112034488 [Quercus suber]

4.7e-26

51.26

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        MKKM F++ WV  +M+CISS +YS+LIN  P  +  PTRG+RQGDPLSPYLFLL  EGF  LL + ES+ ++ G  + K  P LTHL FADDS+IF KS+
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQKPPKFQESSGVVRGSFG
         ++  K  E   +   + G
Subjt:  QQKPPKFQESSGVVRGSFG

XP_026384911.1 uncharacterized protein LOC113280510 [Papaver somniferum]

4.7e-26

53.92

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        MK + FS+ W + +MECIS+V+ SIL+N +P   F PTRG+RQGDPLSPY+F+L  E  S  LN+ E+   + GF++ KHCP +THLFFADD +IF+K+R
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQ
        ++
Subjt:  QQ

XP_027169360.1 uncharacterized protein LOC113769076 [Coffea eugenioides]

4.7e-26

58.82

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        M+KM F++ W   +MECISSVSYS +IN   +E   P RGIRQGDPLSPYLFLLY+EGFS LLN+  +   ++G +I++  P LTHLFFADDS+IF K+ 
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQ
         Q
Subjt:  QQ

TrEMBL top hits

e value

%identity

Alignment

A0A2N9EQW7 Reverse transcriptase domain-containing protein

2.5e-25

53.57

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        MKKM F + W+  + ECISSVSYSILIN  P     P+RG+RQGDPLSPYLFLL AEGF +LL + ES  ++ G  + +  P +THLFFADDS++F K+ 
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQKPPKFQESSG
             K Q   G
Subjt:  QQKPPKFQESSG

A0A2N9EVJ2 Reverse transcriptase domain-containing protein

8.7e-26

58.25

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        M KM F+  WV  +MECISSVSYSILIN SPQ   +PTRG+RQGDPLSPYLFLLYAEG + LLN+  +   + G  +++  P LTHLFF DDS++F  +R
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQK
        + +
Subjt:  QQK

A0A2N9G3I8 Reverse transcriptase domain-containing protein

2.5e-25

53.57

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        MKKM F + W+  + ECISSVSYSILIN  P     P+RG+RQGDPLSPYLFLL AEGF +LL + ES  ++ G  + +  P +THLFFADDS++F K+ 
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQKPPKFQESSG
             K Q   G
Subjt:  QQKPPKFQESSG

A0A2N9G5I8 Reverse transcriptase domain-containing protein

1.9e-25

57.28

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        M KM F+  WV  +MECISSVSYSILIN SPQ   +PTRG+RQGDPLSPYLFLL AEG   LL++  +   + G  +++  P LTHLFFADDS++F ++R
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQK
        +++
Subjt:  QQK

A0A2N9IP69 Reverse transcriptase domain-containing protein

3.3e-25

54.13

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Query:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR
        M KM F   WV  +MECIS+VSYS+LIN  P     P+RG+RQGDP+SPYLFLL AEG + LLN+  S  ++ G  I +  P LTHLFFADDS++F ++ 
Subjt:  MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSR

Query:  QQKPPKFQE
        Q +  K QE
Subjt:  QQKPPKFQE

SwissProt top hits

e value

%identity

Alignment

P92555 Uncharacterized mitochondrial protein AtMg01250

2.4e-12

47.06

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Query:  LINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDS
        +IN +PQ    P+RG+RQGDPLSPYLF+L  E  S L  R +    L G +++ + P + HL FADD+
Subjt:  LINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDS

Arabidopsis top hits

e value

%identity

Alignment

ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase)

1.7e-13

47.06

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Query:  LINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDS
        +IN +PQ    P+RG+RQGDPLSPYLF+L  E  S L  R +    L G +++ + P + HL FADD+
Subjt:  LINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDS

Sequences

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CDS sequence

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ATGAAGAAAATGAATTTTAGTGAAGATTGGGTTAAAAAGGTCATGGAATGTATATCATCGGTTAGCTACTCCATCCTGATCAATGACTCGCCTCAGGAGGAATTTAGGCC
AACCAGAGGAATCAGACAAGGAGACCCTCTATCGCCTTATTTGTTTCTCCTCTACGCGGAAGGATTCTCGGCTCTTTTGAACAGGGATGAATCTTTATCTAACCTTTCTG
GTTTTCAAATTAATAAGCACTGTCCTGTTTTGACCCATCTTTTCTTTGCAGACGACAGCATGATTTTCTTGAAAAGTAGACAACAAAAACCTCCAAAATTTCAAGAAAGT
TCTGGGGTGGTACGAGGAAGCTTCGGGCCAGACCATAAATCTTGA

mRNA sequence

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Protein sequence

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MKKMNFSEDWVKKVMECISSVSYSILINDSPQEEFRPTRGIRQGDPLSPYLFLLYAEGFSALLNRDESLSNLSGFQINKHCPVLTHLFFADDSMIFLKSRQQKPPKFQES
SGVVRGSFGPDHKS

Lag0013731 (gene) of Sponge gourd (AG-4) v1 genome