CuGenDBv2

Gene ID	Lag0013732
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr1:52323163..52323910
RNA-Seq Expression	Lag0013732
Synteny	Lag0013732
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022143535.1 uncharacterized protein LOC111013412 [Momordica charantia]

4.8e-18

38.76

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK
        WKLN++AA+ ++   GG+GW++RD +  V  A CR +  +  +  LE+MA+ EGL+A   I +   RPI ++ DSLE ++LL  +  D TEI   + E  
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK

Query:  SLIKNVGVPFIQHIRRNQNTLAHQVARRA
         ++K++ +  ++HI R  N +AH +ARRA
Subjt:  SLIKNVGVPFIQHIRRNQNTLAHQVARRA

XP_022148737.1 uncharacterized protein LOC111017329 [Momordica charantia]

2.9e-15

32.35

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK
        WKLN+DAA+++ +  GGLGW++R+ +R ++ AG + +T   ++  LE+MA+  G++A++    S    + ++ +SLE ++L++G   ++TEI   V++ K
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK

Query:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTE
        +  +   +   QH++R+ N++A ++A RA+   V E
Subjt:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTE

XP_022155262.1 uncharacterized protein LOC111022403 [Momordica charantia]

1.0e-12

34.06

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK
        W LN+DA+++    +GG+GW++R     + +AG R V     VK+LE  A++EGL+ L ++     RP+ ++ DS EV +LL  +  DLT+    V E  
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK

Query:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERW
        +L  +  +     + R  N  AH +A+RAS L  +  W
Subjt:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERW

XP_022156777.1 uncharacterized protein LOC111023608 [Momordica charantia]

8.2e-18

37.31

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALID-----IPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPF
        WKLN+DAA+ ++   GG+GW++RD +  V  A CR +  +  +  LE+MA+ EGL+A+       I +   RPI ++ DSLE ++LL  +  D TEI   
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALID-----IPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPF

Query:  VRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRA
        + E   +++++ +  ++HI R  N +AH +ARRA
Subjt:  VRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRA

XP_025877173.1 uncharacterized protein LOC107278050 isoform X1 [Oryza sativa Japonica Group]

7.9e-13

32.87

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Query:  KLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETKS
        KLN D +++++R +GG+G ++RD+      A C+ +         EI A +EGL   +   +   RPI ++ D + +VNLLK    DL+E+A  V+E K 
Subjt:  KLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETKS

Query:  LIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERWREPQLN
        L+       +  I R+QN ++H +A RA   S++  W E   N
Subjt:  LIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERWREPQLN

TrEMBL top hits

e value

%identity

Alignment

A0A6J1CP26 uncharacterized protein LOC111013412

2.3e-18

38.76

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK
        WKLN++AA+ ++   GG+GW++RD +  V  A CR +  +  +  LE+MA+ EGL+A   I +   RPI ++ DSLE ++LL  +  D TEI   + E  
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK

Query:  SLIKNVGVPFIQHIRRNQNTLAHQVARRA
         ++K++ +  ++HI R  N +AH +ARRA
Subjt:  SLIKNVGVPFIQHIRRNQNTLAHQVARRA

A0A6J1D5W1 uncharacterized protein LOC111017329

1.4e-15

32.35

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK
        WKLN+DAA+++ +  GGLGW++R+ +R ++ AG + +T   ++  LE+MA+  G++A++    S    + ++ +SLE ++L++G   ++TEI   V++ K
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK

Query:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTE
        +  +   +   QH++R+ N++A ++A RA+   V E
Subjt:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTE

A0A6J1DNV9 uncharacterized protein LOC111022403

5.0e-13

34.06

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK
        W LN+DA+++    +GG+GW++R     + +AG R V     VK+LE  A++EGL+ L ++     RP+ ++ DS EV +LL  +  DLT+    V E  
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETK

Query:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERW
        +L  +  +     + R  N  AH +A+RAS L  +  W
Subjt:  SLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERW

A0A6J1DSV1 uncharacterized protein LOC111023608

4.0e-18

37.31

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Query:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALID-----IPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPF
        WKLN+DAA+ ++   GG+GW++RD +  V  A CR +  +  +  LE+MA+ EGL+A+       I +   RPI ++ DSLE ++LL  +  D TEI   
Subjt:  WKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALID-----IPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPF

Query:  VRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRA
        + E   +++++ +  ++HI R  N +AH +ARRA
Subjt:  VRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRA

A0A803QK79 Uncharacterized protein

3.1e-15

29.63

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Query:  MSEFRPISLCNVIYKIIAKVIANIMKRVIDSIISPTQAAFVSGRQITDNVIVGFEYHQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDC
        M++FRPI+LCNVIYKI++KV+AN  K V+  +I  +Q+AF+ GR I DN+++ FE   + K  S         +  +            ++ C+P  G  
Subjt:  MSEFRPISLCNVIYKIIAKVIANIMKRVIDSIISPTQAAFVSGRQITDNVIVGFEYHQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDC

Query:  EVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRAS
          ++ E M + E L  L    +     + ++ D L VV  ++      +     + + K+L+K+V    I H++R+ N++AH + R +S
Subjt:  EVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRAS

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

2.9e-05

24.68

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Query:  SGRQITDNVIVGFEY--HQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLK-ALIDIPESQRRPIEVDCDSLEVV
        SG Q+  N+ V ++   +Q  K N+DA +  E  + G+GW++R+    V   G R +     V    + A +E L+ A++ +     + I  + D+  +V
Subjt:  SGRQITDNVIVGFEY--HQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLK-ALIDIPESQRRPIEVDCDSLEVV

Query:  NLLKGEGHDLTEIAPFVRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRASTLS
        NLL  +    T + P + + + L+ +      +   R  N +A ++AR + + S
Subjt:  NLLKGEGHDLTEIAPFVRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRASTLS

AT4G29090.1 Ribonuclease H-like superfamily protein

6.9e-07

26.52

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Query:  HQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVR
        HQ  K N+DA +N + ++ G+GWV+R+ +  V+  G R +     V   E+ AM     A++ +   Q   +  + DS  ++ +L  +      + P ++
Subjt:  HQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAMIEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVR

Query:  ETKSLIKNVGVPFIQHIRRNQNTLAHQVARRA
        + + L+          I R  NTLA +VAR +
Subjt:  ETKSLIKNVGVPFIQHIRRNQNTLAHQVARRA

Sequences

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CDS sequence

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ATGTCAGAATTCAGGCCTATTAGCTTATGCAACGTCATCTACAAGATAATTGCAAAAGTAATAGCGAACATAATGAAAAGGGTTATAGATTCAATAATATCCCCGACTCA
AGCCGCTTTTGTGTCGGGAAGACAAATCACGGACAACGTTATAGTGGGATTTGAGTACCATCAACATTGGAAATTAAACTCGGACGCAGCGTACAATTCCGAAAGAAAGC
AAGGCGGCCTTGGATGGGTTGTGCGCGACCATCAACGACGTGTTCGAATGGCGGGTTGTCGTCCTGTTACTGGCGACTGTGAGGTCAAGGTGCTGGAAATTATGGCCATG
ATCGAAGGTCTAAAAGCTTTGATTGATATTCCTGAGAGCCAAAGGAGACCGATTGAAGTGGATTGTGATTCCTTAGAGGTGGTGAATCTTCTCAAAGGGGAAGGGCATGA
TCTTACTGAAATTGCTCCTTTTGTGAGAGAAACCAAGTCCCTAATAAAGAATGTTGGAGTCCCCTTCATTCAGCATATCCGAAGAAATCAGAACACTCTTGCCCATCAGG
TTGCACGAAGGGCTTCGACTTTATCAGTGACTGAACGATGGAGGGAACCTCAGCTCAACATCTTTCTATTGCTGTTTTCTGATTTTTGCTGA

mRNA sequence

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Protein sequence

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MSEFRPISLCNVIYKIIAKVIANIMKRVIDSIISPTQAAFVSGRQITDNVIVGFEYHQHWKLNSDAAYNSERKQGGLGWVVRDHQRRVRMAGCRPVTGDCEVKVLEIMAM
IEGLKALIDIPESQRRPIEVDCDSLEVVNLLKGEGHDLTEIAPFVRETKSLIKNVGVPFIQHIRRNQNTLAHQVARRASTLSVTERWREPQLNIFLLLFSDFC

Lag0013732 (gene) of Sponge gourd (AG-4) v1 genome