CuGenDBv2

Gene ID	Lag0017118
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	RNase H domain-containing protein
Genome location	chr5:321790..322977
RNA-Seq Expression	Lag0017118
Synteny	Lag0017118
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022143317.1 uncharacterized protein LOC111013216 [Momordica charantia]

1.1e-16

33.51

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Query:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVD-------DDPQML--
        R  WT+ D     WN   +  S E+ +  S++I WQIW  RN      +  + +QLD  I  + +  + +  T +   RR    D       ++  ML  
Subjt:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVD-------DDPQML--

Query:  LWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVEAPA-LRIESDAREVVKLLRDEDED
         W   P   WKLN+DA W E+   GGIGWI+ D +G+ V AG  K+ +K +I+ LE   I  GL+ I +++ + + +ESD+ EV++L++ ED D
Subjt:  LWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVEAPA-LRIESDAREVVKLLRDEDED

XP_022143535.1 uncharacterized protein LOC111013412 [Momordica charantia]

7.1e-24

37.7

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Query:  DASGEDRMKRSLIICWQIWCFRNEERNNKQIY---NPEQLDNKIQRYCDELLTNH------------NTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLN
        D +GE+  +RS+II WQIW     E  NK I+   +PE  D  IQ   D  + N             N  L L RR+    +D     W P     WKLN
Subjt:  DASGEDRMKRSLIICWQIWCFRNEERNNKQIY---NPEQLDNKIQRYCDELLTNH------------NTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLN

Query:  SDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLLRDEDEDWSEISNFIKE
        ++A WR D N GGIGWI+RD+KG+ ++A C+ +  +  I++LE  AICEGL++I  E    + +ESD+ E + LL  + +D +EI   ++E
Subjt:  SDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLLRDEDEDWSEISNFIKE

XP_022154990.1 uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]

1.9e-21

35.14

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Query:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTNHNTHLPLARRVSAVDDDPQMLLWI
        R  WT+ +Y E +      D +GE+  +RS+II  QIW  RN+            +   I RY       D  L   +      RR+     D     W 
Subjt:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTNHNTHLPLARRVSAVDDDPQMLLWI

Query:  PAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLL
        P     WKLN+DA WR D N  GIGWI+RD+KG+ ++ GC+ +  +  I++LE  AICEGL++I  E    + +ESD+ E + LL
Subjt:  PAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLL

XP_022154991.1 uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]

1.1e-21

33.01

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Query:  FLFRESNLFLTGSQLPC--SKTHSRAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTN
        + F +S + L   +  C  ++   R  WT+ +Y E +      D +GE+  +RS+II  QIW  RN+            +   I RY       D  L  
Subjt:  FLFRESNLFLTGSQLPC--SKTHSRAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTN

Query:  HNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIES
         +      RR+     D     W P     WKLN+DA WR D N  GIGWI+RD+KG+ ++ GC+ +  +  I++LE  AICEGL++I  E    + +ES
Subjt:  HNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIES

Query:  DAREVVKLL
        D+ E + LL
Subjt:  DAREVVKLL

XP_022156777.1 uncharacterized protein LOC111023608 [Momordica charantia]

2.7e-23

35.15

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Query:  DASGEDRMKRSLIICWQIWCFRNE--------ERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWRE
        D +GE+  +RS+II WQIW  RN+        E  + Q+     + N   R  +    + N  L L RR+     D     W P     WKLN+DA WR 
Subjt:  DASGEDRMKRSLIICWQIWCFRNE--------ERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWRE

Query:  DLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSI------PVEAPALR---IESDAREVVKLLRDEDEDWSEISNFIKEAKALILSK
        D N GGIGWI+RD+KG+ ++A C+ +  +  I++LE  AICEGL++I      P++    R   +ESD+ E + LL  + +D +EI   ++E   ++   
Subjt:  DLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSI------PVEAPALR---IESDAREVVKLLRDEDEDWSEISNFIKEAKALILSK

Query:  KI
        KI
Subjt:  KI

TrEMBL top hits

e value

%identity

Alignment

A0A6J1CP26 uncharacterized protein LOC111013412

3.4e-24

37.7

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Query:  DASGEDRMKRSLIICWQIWCFRNEERNNKQIY---NPEQLDNKIQRYCDELLTNH------------NTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLN
        D +GE+  +RS+II WQIW     E  NK I+   +PE  D  IQ   D  + N             N  L L RR+    +D     W P     WKLN
Subjt:  DASGEDRMKRSLIICWQIWCFRNEERNNKQIY---NPEQLDNKIQRYCDELLTNH------------NTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLN

Query:  SDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLLRDEDEDWSEISNFIKE
        ++A WR D N GGIGWI+RD+KG+ ++A C+ +  +  I++LE  AICEGL++I  E    + +ESD+ E + LL  + +D +EI   ++E
Subjt:  SDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLLRDEDEDWSEISNFIKE

A0A6J1CQG0 uncharacterized protein LOC111013216

5.3e-17

33.51

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Query:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVD-------DDPQML--
        R  WT+ D     WN   +  S E+ +  S++I WQIW  RN      +  + +QLD  I  + +  + +  T +   RR    D       ++  ML  
Subjt:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVD-------DDPQML--

Query:  LWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVEAPA-LRIESDAREVVKLLRDEDED
         W   P   WKLN+DA W E+   GGIGWI+ D +G+ V AG  K+ +K +I+ LE   I  GL+ I +++ + + +ESD+ EV++L++ ED D
Subjt:  LWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVEAPA-LRIESDAREVVKLLRDEDED

A0A6J1DL64 uncharacterized protein LOC111022134 isoform X1

9.4e-22

35.14

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Query:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTNHNTHLPLARRVSAVDDDPQMLLWI
        R  WT+ +Y E +      D +GE+  +RS+II  QIW  RN+            +   I RY       D  L   +      RR+     D     W 
Subjt:  RAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTNHNTHLPLARRVSAVDDDPQMLLWI

Query:  PAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLL
        P     WKLN+DA WR D N  GIGWI+RD+KG+ ++ GC+ +  +  I++LE  AICEGL++I  E    + +ESD+ E + LL
Subjt:  PAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIESDAREVVKLL

A0A6J1DQC9 uncharacterized protein LOC111022134 isoform X2

5.5e-22

33.01

Show/hide

Query:  FLFRESNLFLTGSQLPC--SKTHSRAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTN
        + F +S + L   +  C  ++   R  WT+ +Y E +      D +GE+  +RS+II  QIW  RN+            +   I RY       D  L  
Subjt:  FLFRESNLFLTGSQLPC--SKTHSRAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYC------DELLTN

Query:  HNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIES
         +      RR+     D     W P     WKLN+DA WR D N  GIGWI+RD+KG+ ++ GC+ +  +  I++LE  AICEGL++I  E    + +ES
Subjt:  HNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVE-APALRIES

Query:  DAREVVKLL
        D+ E + LL
Subjt:  DAREVVKLL

A0A6J1DSV1 uncharacterized protein LOC111023608

1.3e-23

35.15

Show/hide

Query:  DASGEDRMKRSLIICWQIWCFRNE--------ERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWRE
        D +GE+  +RS+II WQIW  RN+        E  + Q+     + N   R  +    + N  L L RR+     D     W P     WKLN+DA WR 
Subjt:  DASGEDRMKRSLIICWQIWCFRNE--------ERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQMLLWIPAPKGVWKLNSDACWRE

Query:  DLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSI------PVEAPALR---IESDAREVVKLLRDEDEDWSEISNFIKEAKALILSK
        D N GGIGWI+RD+KG+ ++A C+ +  +  I++LE  AICEGL++I      P++    R   +ESD+ E + LL  + +D +EI   ++E   ++   
Subjt:  DLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSI------PVEAPALR---IESDAREVVKLLRDEDEDWSEISNFIKEAKALILSK

Query:  KI
        KI
Subjt:  KI

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

6.5e-07

24.14

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Query:  WTSADYLELIWNESSSDASGEDRMKRSLI--ICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQMLLWIPAPKGVW
        WT + Y  L W  +      +     +L+  + W++W  RNE     + Y+  +    + R   E     +T   L  + S    +  + +   AP   W
Subjt:  WTSADYLELIWNESSSDASGEDRMKRSLI--ICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQMLLWIPAPKGVW

Query:  -KLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKI--SWLEA--WAICEGLKSIPVEAPALRIESDAREVVKLLRDEDEDWSEISNFIKEAK
         K N+DA W+ +    GIGWI+R++ G  +  G + + +   +  + LEA  WA+   L         +  ESDA+ +V LL + D+ W  +   +++ +
Subjt:  -KLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKI--SWLEA--WAICEGLKSIPVEAPALRIESDAREVVKLLRDEDEDWSEISNFIKEAK

Query:  ALI
         L+
Subjt:  ALI

AT4G29090.1 Ribonuclease H-like superfamily protein

1.4e-09

24.2

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Query:  WTSADYLELIW--NESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQML-----LWIPA
        W  + Y+ L W  N  + +   E   +    + W++W  RNE     + +N +++   ++R  D+L         +     +    PQ+       W P 
Subjt:  WTSADYLELIW--NESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLARRVSAVDDDPQML-----LWIPA

Query:  PKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKI--SWLEA--WAICEGLKSIPVEAPALRIESDAREVVKLLRDEDEDWSEISNFI
        P    K N+DA W  D    GIGW++R++KG+    G + + K   +  + LEA  WA+   L     +   +  ESD++ ++++L + DE W  +   I
Subjt:  PKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKI--SWLEA--WAICEGLKSIPVEAPALRIESDAREVVKLLRDEDEDWSEISNFI

Query:  KEAKALILSKKIEKIVQMP
        ++ + L+      K V +P
Subjt:  KEAKALILSKKIEKIVQMP

Sequences

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CDS sequence

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ATGTACAACTCCTTTCTCTTCAGGGAATCCAATCTATTTCTCACAGGCTCACAGCTACCTTGTTCAAAAACCCATTCTAGGGCAGGGTGGACCTCGGCTGACTAC
CTGGAATTAATCTGGAACGAAAGCAGCAGCGATGCAAGCGGAGAAGATAGGATGAAGAGGAGCTTAATCATATGCTGGCAAATATGGTGTTTTCGAAATGAAGAG
AGAAACAATAAGCAGATCTACAATCCGGAGCAGTTGGACAACAAAATCCAGAGATATTGCGATGAATTGTTAACAAACCACAATACGCACCTTCCTTTGGCAAGG
AGAGTATCTGCCGTCGACGATGATCCTCAGATGTTGCTTTGGATACCAGCGCCGAAAGGAGTCTGGAAACTCAATAGTGATGCGTGTTGGAGGGAGGATTTAAAT
TGTGGGGGAATAGGCTGGATCATTCGGGATGACAAGGGTCAACCGGTGAGAGCAGGCTGCAAAAAGGTGGATAAAAAATGGAAAATCTCGTGGCTTGAGGCGTGG
GCAATTTGCGAGGGGCTGAAATCGATTCCGGTGGAGGCTCCTGCTCTGCGAATAGAGTCAGATGCTCGAGAAGTGGTGAAATTATTGAGGGATGAGGATGAAGAT
TGGTCTGAGATATCCAACTTTATTAAGGAAGCAAAAGCCCTAATCTTGTCTAAGAAGATTGAGAAGATAGTTCAGATGCCTGTGTTTGGAGTGCTGTATTTGGGC
TGTAATCTGACCGATGCCTGTGTTTGGAGTGCTGTATTTGGGTTGTAA

mRNA sequence

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Protein sequence

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MYNSFLFRESNLFLTGSQLPCSKTHSRAGWTSADYLELIWNESSSDASGEDRMKRSLIICWQIWCFRNEERNNKQIYNPEQLDNKIQRYCDELLTNHNTHLPLAR
RVSAVDDDPQMLLWIPAPKGVWKLNSDACWREDLNCGGIGWIIRDDKGQPVRAGCKKVDKKWKISWLEAWAICEGLKSIPVEAPALRIESDAREVVKLLRDEDED
WSEISNFIKEAKALILSKKIEKIVQMPVFGVLYLGCNLTDACVWSAVFGL

Lag0017118 (gene) of Sponge gourd (AG-4) v1 genome