CuGenDBv2

Gene ID	Lag0018136
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	DPH4 homolog
Genome location	chr5:17276603..17278387
RNA-Seq Expression	Lag0018136
Synteny	Lag0018136
Gene Ontology terms	GO:0002098 - tRNA wobble uridine modification (biological process) GO:0017183 - peptidyl-diphthamide biosynthetic process from peptidyl-histidine (biological process) GO:0005634 - nucleus (cellular component) GO:0005829 - cytosol (cellular component) GO:0046872 - metal ion binding (molecular function)
InterPro domains	IPR007872 - DPH-type metal-binding domain IPR036671 - DPH-type metal-binding domain superfamily IPR036869 - Chaperone J-domain superfamily IPR044248 - Diphthamide biosynthesis protein 3/4-like

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022144178.1 DPH4 homolog [Momordica charantia]

4.7e-52

74.38

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSS  G IGETYY VLSL+ DASYDEIRASYR                              VQKAWEVL +STSRA YDRELQAAKGDAMGAESISLED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        MVVEDK EVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTL+ALPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

XP_022962179.1 DPH4 homolog [Cucurbita moschata]

8.0e-52

73.75

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSGSIGETYY VLSLREDASYDEIRASYR                              VQKAWEVL +S SRA YDRELQAAKGDA+GAESI LED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        M VEDK EVVELFYQCRCGDYFFIDSGEL+EMGYPLLRNG KVSLRTLD LPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

XP_022996470.1 DPH4 homolog [Cucurbita maxima]

8.0e-52

73.75

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSGSIGETYY VLSLREDASYDEIRASYR                              VQKAWEVL +S SRA YDRELQAAKGDA+GAESI LED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        M VEDK EVVELFYQCRCGDYFFIDSGEL+EMGYPLLRNG KVSLRTLD LPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

XP_038884887.1 DPH4 homolog isoform X1 [Benincasa hispida]

1.5e-53

75.62

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSGSIGETYY VLSL+EDASYDEIRASYR                              VQKAWEVL +S SRA+YDRELQAAKGDA+GAESISLED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        MVVEDK EVVELFYQCRCGDYFFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

XP_038884890.1 DPH4 homolog isoform X2 [Benincasa hispida]

1.5e-53

75.62

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSGSIGETYY VLSL+EDASYDEIRASYR                              VQKAWEVL +S SRA+YDRELQAAKGDA+GAESISLED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        MVVEDK EVVELFYQCRCGDYFFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BBM0 DPH4 homolog

8.6e-52

73.75

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSG+IGETYY VLSLREDAS+DEIRASYR                              VQKAWEVL +  SRA+YDRELQAAKGDA+GAESISLED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        MVVEDK EVVELFYQCRCGD FFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

A0A5A7V202 DPH4-like protein

8.6e-52

73.75

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSG+IGETYY VLSLREDAS+DEIRASYR                              VQKAWEVL +  SRA+YDRELQAAKGDA+GAESISLED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        MVVEDK EVVELFYQCRCGD FFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

A0A6J1CSL8 DPH4 homolog

2.3e-52

74.38

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSS  G IGETYY VLSL+ DASYDEIRASYR                              VQKAWEVL +STSRA YDRELQAAKGDAMGAESISLED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        MVVEDK EVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTL+ALPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

A0A6J1HGB5 DPH4 homolog

3.9e-52

73.75

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSGSIGETYY VLSLREDASYDEIRASYR                              VQKAWEVL +S SRA YDRELQAAKGDA+GAESI LED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        M VEDK EVVELFYQCRCGDYFFIDSGEL+EMGYPLLRNG KVSLRTLD LPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

A0A6J1K4U3 DPH4 homolog

3.9e-52

73.75

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Query:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED
        MSSSSGSIGETYY VLSLREDASYDEIRASYR                              VQKAWEVL +S SRA YDRELQAAKGDA+GAESI LED
Subjt:  MSSSSGSIGETYY-VLSLREDASYDEIRASYR------------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLED

Query:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        M VEDK EVVELFYQCRCGDYFFIDSGEL+EMGYPLLRNG KVSLRTLD LPASVVLPCG
Subjt:  MVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

SwissProt top hits

e value

%identity

Alignment

Q5SNN4 Zinc finger CCCH domain-containing protein 40

2.0e-05

56.41

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEMPSL PP+DESIRTLY+GGL+ ++TE
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

Q6Z358 Zinc finger CCCH domain-containing protein 49

1.1e-06

61.54

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEMPSL PP+DESIRTLY+GGLD++VTE
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

Q9FL40 Zinc finger CCCH domain-containing protein 53

1.3e-04

53.85

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEM +LEPPEDESI+TLYVGGL++++ E
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

Arabidopsis top hits

e value

%identity

Alignment

AT1G07360.1 CCCH-type zinc fingerfamily protein with RNA-binding domain

2.3e-04

48.72

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEM +LE P+DESI+TLYVGGL++++ E
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

AT2G29580.1 CCCH-type zinc fingerfamily protein with RNA-binding domain

7.8e-05

53.85

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEM +LE PED+SIRTLYVGGL+++V E
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

AT4G10130.1 DNAJ heat shock N-terminal domain-containing protein

6.3e-31

46.98

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Query:  IGETYY-VLSLREDASYDEIRASYR--------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLEDMVVEDKREVVE
        + ETYY +LS++EDASY+EIR SYR                          +QKAWEVL ++  R  YD +L++++ D + A+ IS+EDM VE   +V++
Subjt:  IGETYY-VLSLREDASYDEIRASYR--------------------------VQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLEDMVVEDKREVVE

Query:  LFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG
        LFYQCRCGDYF +DS EL  MG+ LLR+G  V ++ L A  ASVVLPCG
Subjt:  LFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCG

AT5G07060.1 CCCH-type zinc fingerfamily protein with RNA-binding domain

9.2e-06

53.85

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEM +LEPPEDESI+TLYVGGL++++ E
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

AT5G07060.2 CCCH-type zinc fingerfamily protein with RNA-binding domain

9.2e-06

53.85

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Query:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE
        D +   ++   GEM +LEPPEDESI+TLYVGGL++++ E
Subjt:  DALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTE

Sequences

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CDS sequence

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ATGAGTTCAAGCAGTGGCTCTATTGGTGAAACTTATTATGTACTGTCTTTGAGAGAAGATGCCAGCTATGACGAAATTCGAGCCAGCTACCGGGTGCAGAAGGCTTGGGA
AGTCCTCGACAACTCAACATCTCGTGCAACTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGGTGCAGAGAGCATAAGCTTAGAAGACATGGTTGTGGAAG
ATAAACGTGAAGTTGTAGAACTCTTTTATCAGTGTCGCTGTGGTGATTACTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGT
AAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGAGAGATGCCTTCCCTAGAACCTCCAGAGGATGAGAGTATCAGAACCTTGTATGT
GGGTGGACTTGATGCTCAAGTCACCGAACTAATCTGCGAAGTCCACCCTTCAAGTAGGTATATGTTAAGTGTGCTCAACAATCGGGATCTACGGAGTGTGGACACTACAC
TCTTAAATTTATGCGAGATATGGTATCCCATAGGAGCAGAGTGA

mRNA sequence

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Protein sequence

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MSSSSGSIGETYYVLSLREDASYDEIRASYRVQKAWEVLDNSTSRATYDRELQAAKGDAMGAESISLEDMVVEDKREVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGS
KVSLRTLDALPASVVLPCGEMPSLEPPEDESIRTLYVGGLDAQVTELICEVHPSSRYMLSVLNNRDLRSVDTTLLNLCEIWYPIGAE

Lag0018136 (gene) of Sponge gourd (AG-4) v1 genome