CuGenDBv2

Gene ID	Lag0019039
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	RNase H domain-containing protein
Genome location	chr5:37903783..37904256
RNA-Seq Expression	Lag0019039
Synteny	Lag0019039
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022143535.1 uncharacterized protein LOC111013412 [Momordica charantia]

5.3e-14

32.7

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFI-----KQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVV
        M +  ++WE RN+ IF      T+D+  +I  +I     +  +    S NK  H    +E  +G      W PP  N WKLN++A +  +    G+G ++
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFI-----KQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVV

Query:  RDHQGQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
        RD +G+V  A CR I  E  +  LE  A+ EGL+A+ +       P+ +ESDSLE  H+
Subjt:  RDHQGQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

XP_022154990.1 uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]

1.3e-15

34.19

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ
        M +  ++WE RN+ IF      T+D+  +I  +I  ++   +++  KSK   P+     G +T   W PP  N WKLN+DA +  +    G+G ++RD +
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ

Query:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
        G+V   GCR I  E  +  LE  A+ EGL+A+ +       P+ +ESDSLE  H+
Subjt:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

XP_022154991.1 uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]

1.3e-15

34.19

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ
        M +  ++WE RN+ IF      T+D+  +I  +I  ++   +++  KSK   P+     G +T   W PP  N WKLN+DA +  +    G+G ++RD +
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ

Query:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
        G+V   GCR I  E  +  LE  A+ EGL+A+ +       P+ +ESDSLE  H+
Subjt:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

XP_022155262.1 uncharacterized protein LOC111022403 [Momordica charantia]

9.3e-11

29.05

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Query:  LWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQVRLAG
        +W  RN +IF         +I  ++ F+ ++S  S +     HK          +  L W PP  + W LN+DA++S    + G+G ++R   G + LAG
Subjt:  LWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQVRLAG

Query:  CRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
         R +     VK+LE  A++EGL+ L  +  ++  P+ +E+DS E++ +
Subjt:  CRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

XP_022156777.1 uncharacterized protein LOC111023608 [Momordica charantia]

3.1e-14

33.12

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ
        M +  ++WE RN+ IF      T+D+   I  +I  ++   +++  KS +K   +    G +T   W PP  N WKLN+DA +  +    G+G ++RD +
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ

Query:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLK-----IPAIQRNPVGVESDSLEIQHI
        G+V  A CR I  E  +  LE  A+ EGL+A+ +     I      P+ +ESDSLE  H+
Subjt:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLK-----IPAIQRNPVGVESDSLEIQHI

TrEMBL top hits

e value

%identity

Alignment

A0A6J1CP26 uncharacterized protein LOC111013412

2.6e-14

32.7

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFI-----KQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVV
        M +  ++WE RN+ IF      T+D+  +I  +I     +  +    S NK  H    +E  +G      W PP  N WKLN++A +  +    G+G ++
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFI-----KQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVV

Query:  RDHQGQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
        RD +G+V  A CR I  E  +  LE  A+ EGL+A+ +       P+ +ESDSLE  H+
Subjt:  RDHQGQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

A0A6J1DL64 uncharacterized protein LOC111022134 isoform X1

6.1e-16

34.19

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ
        M +  ++WE RN+ IF      T+D+  +I  +I  ++   +++  KSK   P+     G +T   W PP  N WKLN+DA +  +    G+G ++RD +
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ

Query:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
        G+V   GCR I  E  +  LE  A+ EGL+A+ +       P+ +ESDSLE  H+
Subjt:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

A0A6J1DNV9 uncharacterized protein LOC111022403

4.5e-11

29.05

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Query:  LWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQVRLAG
        +W  RN +IF         +I  ++ F+ ++S  S +     HK          +  L W PP  + W LN+DA++S    + G+G ++R   G + LAG
Subjt:  LWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQVRLAG

Query:  CRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
         R +     VK+LE  A++EGL+ L  +  ++  P+ +E+DS E++ +
Subjt:  CRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

A0A6J1DQC9 uncharacterized protein LOC111022134 isoform X2

6.1e-16

34.19

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ
        M +  ++WE RN+ IF      T+D+  +I  +I  ++   +++  KSK   P+     G +T   W PP  N WKLN+DA +  +    G+G ++RD +
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ

Query:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI
        G+V   GCR I  E  +  LE  A+ EGL+A+ +       P+ +ESDSLE  H+
Subjt:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHI

A0A6J1DSV1 uncharacterized protein LOC111023608

1.5e-14

33.12

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Query:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ
        M +  ++WE RN+ IF      T+D+   I  +I  ++   +++  KS +K   +    G +T   W PP  N WKLN+DA +  +    G+G ++RD +
Subjt:  MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSI-NKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQ

Query:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLK-----IPAIQRNPVGVESDSLEIQHI
        G+V  A CR I  E  +  LE  A+ EGL+A+ +     I      P+ +ESDSLE  H+
Subjt:  GQVRLAGCRAISGECEVKVLETKALIEGLKALLK-----IPAIQRNPVGVESDSLEIQHI

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.1e-04

24.59

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Query:  LCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQV
        L  +LW++RN+++F   +    +++       ++ S+      + K   P +E    ++  + W  P     K N+DAT+ +E  + G+G ++R+  G V
Subjt:  LCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQV

Query:  RLAGCRAISGECEVKVLETKAL
           G RA+     V   E +AL
Subjt:  RLAGCRAISGECEVKVLETKAL

AT4G29090.1 Ribonuclease H-like superfamily protein

3.5e-08

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Query:  LCGKLWETRNQIIFGDSKCHTKDLI----DSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDH
        L  +LW+ RN+++F   + + ++++    D +  +  +  + S       ++          S+C  W PP     K N+DAT++ + ++ G+G V+R+ 
Subjt:  LCGKLWETRNQIIFGDSKCHTKDLI----DSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDH

Query:  QGQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDS
        +G+V+  G RA+     V   E +A+     A+L +   Q N V  ESDS
Subjt:  QGQVRLAGCRAISGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDS

Sequences

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CDS sequence

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ATGCGACTTTGTGGAAAATTATGGGAGACTCGGAACCAAATCATTTTTGGAGATTCAAAGTGCCACACGAAGGATTTGATAGACTCCATTTCTACATTCATCAAGCAGAA
CAGTTCCAACAGTTCAAGCATTAACAAAAGCAAGCATAAATTTCCTTTAATGGAGGCAGGAAGTGGTCAAAGTACCTGTTTGAGCTGGAACCCACCTGATAAAAATCATT
GGAAGCTGAACTCGGACGCGACGTACAGTGTTGAGCGTAAGCAAGCGGGTTTAGGAGGGGTAGTGCGCGACCATCAAGGGCAGGTCCGTCTAGCGGGTTGTCGAGCTATT
TCAGGCGAGTGTGAAGTCAAAGTTCTGGAAACCAAGGCCTTAATCGAAGGGCTCAAAGCTCTTTTGAAAATTCCTGCAATACAAAGGAATCCAGTCGGTGTGGAGAGCGA
CTCTTTGGAGATCCAACATATCAGAAGAACCTAG

mRNA sequence

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Protein sequence

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MRLCGKLWETRNQIIFGDSKCHTKDLIDSISTFIKQNSSNSSSINKSKHKFPLMEAGSGQSTCLSWNPPDKNHWKLNSDATYSVERKQAGLGGVVRDHQGQVRLAGCRAI
SGECEVKVLETKALIEGLKALLKIPAIQRNPVGVESDSLEIQHIRRT

Lag0019039 (gene) of Sponge gourd (AG-4) v1 genome