CuGenDBv2

Gene ID	Lag0019043
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase
Genome location	chr5:37959128..37959517
RNA-Seq Expression	Lag0019043
Synteny	Lag0019043
Gene Ontology terms	GO:0090304 - nucleic acid metabolic process (biological process) GO:0016020 - membrane (cellular component) GO:0005488 - binding (molecular function) GO:0140640 - catalytic activity, acting on a nucleic acid (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

ONI01138.1 hypothetical protein PRUPE_6G123900 [Prunus persica]

2.3e-39

58.14

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        MARFW    K  RGIHWV W+ L K K  GG+GFRD+E FNQALLAKQCWRI++ P SL+A + + RY P   FL AEVG+ PSFIWRSL WG+ELL +G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS
        L WR G+G  + VY   WLP    F++ S
Subjt:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS

VVA32947.1 PREDICTED: retrotransposon [Prunus dulcis]

6.0e-40

58.91

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        MARFW    K  RGIHWV W+ L K K  GG+GFRD+E FNQALLAKQCWRI++ P SL+A + + RY P   FL AEVG+ PSFIWRSL WG+ELL +G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS
        L WR GNG  + VY   WLP    F++ S
Subjt:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS

XP_022150918.1 uncharacterized protein LOC111018954 [Momordica charantia]

1.5e-43

58.59

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Query:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL
        ARFW G  K D+ IHWV+W SL  PKC GGMGFRD+E+FN+ALLAKQCWRI+ +P+S+L+ VLKGRYF    F+ A++   PS+IWRS+LWGR+LL++GL
Subjt:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL

Query:  CWRAGNGECVPVYGVNWLPDDGNFRVRS
         WR GNG+ V +YG NW+P+    ++ S
Subjt:  CWRAGNGECVPVYGVNWLPDDGNFRVRS

XP_022159001.1 uncharacterized protein LOC111025446 [Momordica charantia]

3.5e-40

60.16

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Query:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL
        ARFW      D+ IHW+ W +LRKPK  GGMGFRD+E+FN+ALLAKQ WRI+ NP S+LA VLKGRYF   D L+AE+   PSFIWRSL+WG ELL+ GL
Subjt:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL

Query:  CWRAGNGECVPVYGVNWLPDDGNFRVRS
         WR GNGE V VY  NW+P+  + ++ S
Subjt:  CWRAGNGECVPVYGVNWLPDDGNFRVRS

XP_022159057.1 uncharacterized protein LOC111025497 [Momordica charantia]

1.3e-39

61.24

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        MARFW G    D  IHWVSWK +   KC GG+GFRD+E FNQALLAKQ W+I  +PSSLLA VLK +YF   DF+ A VGSR SFIWRSLLWG +LL  G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS
        L WR GNG  + +Y  NW+P DG   V+S
Subjt:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS

TrEMBL top hits

e value

%identity

Alignment

A0A5E4FZN9 PREDICTED: retrotransposon

2.9e-40

58.91

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        MARFW    K  RGIHWV W+ L K K  GG+GFRD+E FNQALLAKQCWRI++ P SL+A + + RY P   FL AEVG+ PSFIWRSL WG+ELL +G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS
        L WR GNG  + VY   WLP    F++ S
Subjt:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS

A0A6J1DAR4 uncharacterized protein LOC111018954

7.4e-44

58.59

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Query:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL
        ARFW G  K D+ IHWV+W SL  PKC GGMGFRD+E+FN+ALLAKQCWRI+ +P+S+L+ VLKGRYF    F+ A++   PS+IWRS+LWGR+LL++GL
Subjt:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL

Query:  CWRAGNGECVPVYGVNWLPDDGNFRVRS
         WR GNG+ V +YG NW+P+    ++ S
Subjt:  CWRAGNGECVPVYGVNWLPDDGNFRVRS

A0A6J1DYS7 uncharacterized protein LOC111025497

6.5e-40

61.24

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        MARFW G    D  IHWVSWK +   KC GG+GFRD+E FNQALLAKQ W+I  +PSSLLA VLK +YF   DF+ A VGSR SFIWRSLLWG +LL  G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS
        L WR GNG  + +Y  NW+P DG   V+S
Subjt:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS

A0A6J1E2L3 uncharacterized protein LOC111025446

1.7e-40

60.16

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Query:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL
        ARFW      D+ IHW+ W +LRKPK  GGMGFRD+E+FN+ALLAKQ WRI+ NP S+LA VLKGRYF   D L+AE+   PSFIWRSL+WG ELL+ GL
Subjt:  ARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGL

Query:  CWRAGNGECVPVYGVNWLPDDGNFRVRS
         WR GNGE V VY  NW+P+  + ++ S
Subjt:  CWRAGNGECVPVYGVNWLPDDGNFRVRS

M5W5F3 Reverse transcriptase domain-containing protein (Fragment)

1.1e-39

58.14

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        MARFW    K  RGIHWV W+ L K K  GG+GFRD+E FNQALLAKQCWRI++ P SL+A + + RY P   FL AEVG+ PSFIWRSL WG+ELL +G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS
        L WR G+G  + VY   WLP    F++ S
Subjt:  LCWRAGNGECVPVYGVNWLPDDGNFRVRS

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

1.1e-09

31.78

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Query:  HWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYF--PFRDFLRAEVGSRPSFIWRSLLWG-RELLERGLCWRAGNGECVP
        H V W  +  PK  GG+G R  +  N+AL++K  WR++Q  +SL   VL+ +Y     RD          S  WRS+  G R+++  G+ W  G+G+ + 
Subjt:  HWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYF--PFRDFLRAEVGSRPSFIWRSLLWG-RELLERGLCWRAGNGECVP

Query:  VYGVNWL
         +   W+
Subjt:  VYGVNWL

P93295 Uncharacterized mitochondrial protein AtMg00310

4.6e-27

47.15

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPK-CYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLER
        M  FW    +  R I WV+W+ L K K   GG+GFRD+  FNQALLAKQ +RII  P +LL+ +L+ RYFP    +   VG+RPS+ WRS++ GRELL R
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPK-CYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLER

Query:  GLCWRAGNGECVPVYGVNWLPDD
        GL    G+G    V+   W+ D+
Subjt:  GLCWRAGNGECVPVYGVNWLPDD

Arabidopsis top hits

e value

%identity

Alignment

AT4G29090.1 Ribonuclease H-like superfamily protein

5.6e-28

44.54

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG
        +A FW   ++  +G+HW +W  L   K  GG+GF+D+E FN ALL KQ WR++  P SL+A V K RYF   D L A +GSRPSF+W+S+   +E+L +G
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERG

Query:  LCWRAGNGECVPVYGVNWL
             GNGE + ++   WL
Subjt:  LCWRAGNGECVPVYGVNWL

ATMG00310.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

3.3e-28

47.15

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Query:  MARFWCGGEKVDRGIHWVSWKSLRKPK-CYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLER
        M  FW    +  R I WV+W+ L K K   GG+GFRD+  FNQALLAKQ +RII  P +LL+ +L+ RYFP    +   VG+RPS+ WRS++ GRELL R
Subjt:  MARFWCGGEKVDRGIHWVSWKSLRKPK-CYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLER

Query:  GLCWRAGNGECVPVYGVNWLPDD
        GL    G+G    V+   W+ D+
Subjt:  GLCWRAGNGECVPVYGVNWLPDD

Sequences

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CDS sequence

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ATGGCCCGATTTTGGTGTGGTGGGGAGAAGGTGGATAGAGGAATTCATTGGGTGAGTTGGAAATCTCTACGTAAGCCTAAATGCTATGGTGGGATGGGTTTTAGGGATAT
GGAGATTTTTAACCAAGCTTTGCTGGCAAAACAGTGTTGGAGAATTATCCAGAATCCATCTTCCCTCTTGGCAAGTGTTTTGAAGGGCAGATATTTTCCTTTCAGAGATT
TCTTGAGAGCAGAAGTGGGGTCGAGACCGTCCTTTATATGGAGGAGTTTGTTGTGGGGGAGAGAGCTTTTGGAGAGGGGCTTGTGTTGGCGAGCTGGGAATGGGGAATGT
GTGCCGGTATATGGAGTGAACTGGCTTCCAGATGATGGTAATTTTAGAGTGCGATCATGA

mRNA sequence

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Protein sequence

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MARFWCGGEKVDRGIHWVSWKSLRKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLASVLKGRYFPFRDFLRAEVGSRPSFIWRSLLWGRELLERGLCWRAGNGEC
VPVYGVNWLPDDGNFRVRS

Lag0019043 (gene) of Sponge gourd (AG-4) v1 genome