CuGenDBv2

Gene ID	Lag0021830
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
Genome location	chr7:12746533..12750570
RNA-Seq Expression	Lag0021830
Synteny	Lag0021830
Gene Ontology terms	GO:0017183 - peptidyl-diphthamide biosynthetic process from peptidyl-histidine (biological process) GO:0016740 - transferase activity (molecular function)
InterPro domains	IPR010014 - Diphthamide synthesis DHP2 IPR016435 - Diphthamide synthesis DPH1/DPH2 IPR042263 - Diphthamide synthesis DPH1/DPH2, domain 1

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6597669.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2, partial [Cucurbita argyrosperma subsp. sororia]

6.8e-47

72.22

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

KAG7029112.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita argyrosperma subsp. argyrosperma]

6.8e-47

72.22

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

XP_022932622.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita moschata]

6.8e-47

72.22

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

XP_022972272.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita maxima]

2.2e-45

70.83

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALND NQSD EN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

XP_023541019.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita pepo subsp. pepo]

6.8e-47

72.22

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BYG9 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

5.5e-42

66.44

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Query:  LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLL
        L+FPDELLKDSTRVV AL+ RLR L  +DTN SD +NEVRLF+MADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                       
Subjt:  LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLL

Query:  IFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                  TTTLPA FVFGKDSIDV+TCAKTLS YSLS+GKPVL
Subjt:  IFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

A0A5A7TMM8 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

5.5e-42

66.44

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Query:  LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLL
        L+FPDELLKDSTRVV AL+ RLR L  +DTN SD +NEVRLF+MADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                       
Subjt:  LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLL

Query:  IFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                  TTTLPA FVFGKDSIDV+TCAKTLS YSLS+GKPVL
Subjt:  IFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

A0A5D3D327 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

5.5e-42

66.44

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Query:  LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLL
        L+FPDELLKDSTRVV AL+ RLR L  +DTN SD +NEVRLF+MADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                       
Subjt:  LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLL

Query:  IFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                  TTTLPA FVFGKDSIDV+TCAKTLS YSLS+GKPVL
Subjt:  IFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

A0A6J1EXA1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

3.3e-47

72.22

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

A0A6J1I4D3 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

1.1e-45

70.83

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDSTRVV+ALR+RL ALND NQSD EN+VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                TTTLPAFFVFGKD IDVSTCAKTLSHYS SNGKPVL
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

SwissProt top hits

e value

%identity

Alignment

A0A1D8PL26 Diphthamide biosynthesis protein 2-2

1.8e-13

46.05

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEV--RLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLS
        L+FPD L+ DS  +V+ L+R L  +   NQ  GE+    R++I+ADT+Y +CCVDEV A H  +D V+H+G  CL+
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEV--RLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLS

A4QN59 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

7.2e-15

35.11

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELL D+ RV             + + + + + + +I+ DT+YGSCCVDEV A H  ADC++HYG +CLSP  R                      
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKT
                   LP  +VFGK  IDV  CA +
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKT

Q4I5M4 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

9.4e-15

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP
        L+FPD +L D+ RVV AL +    +N  +Q + + E R++++AD++Y +CCVDE+ A H +AD V+HYG TCLSP
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP

Q59SJ9 Diphthamide biosynthesis protein 2-1

1.8e-13

46.05

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Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEV--RLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLS
        L+FPD L+ DS  +V+ L+R L  +   NQ  GE+    R++I+ADT+Y +CCVDEV A H  +D V+H+G  CL+
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEV--RLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLS

Q5B2Q1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

6.1e-14

39.83

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Query:  ELHQSRIAGISLNSQILEFPDELLKDSTRVVNALRRRL----------RALNDTNQSDG--------------ENEVRLFIMADTTYGSCCVDEVGAAHA
        E+ Q+R   I+     L+FPDE+L D+ RV   L R L          +A  +T  +DG              E   +L+I+ADT+YG+CCVDEV A H 
Subjt:  ELHQSRIAGISLNSQILEFPDELLKDSTRVVNALRRRL----------RALNDTNQSDG--------------ENEVRLFIMADTTYGSCCVDEVGAAHA

Query:  NADCVIHYGHTCLSPALR
        +AD V+HYG +CLSP  R
Subjt:  NADCVIHYGHTCLSPALR

Arabidopsis top hits

e value

%identity

Alignment

AT3G59630.1 diphthamide synthesis DPH2 family protein

1.1e-29

47.22

Show/hide

Query:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF
        L+FPDELLKDST+VV+AL+ + R L D        EVR F+MADTTYGSCC+DEVGA H +++CV+HYG TCLSP                         
Subjt:  LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIF

Query:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL
                T+ LPAFFVFGK SI+VS+C K L  Y+  + KP++
Subjt:  LHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVL

AT5G62030.1 diphthamide synthesis DPH2 family protein

1.3e-08

25.3

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Query:  FIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSN
        F++ D TYG+CCVD+  A    AD +IHYGH+CL P                               + +T +P  +VF +  IDV     T+ H +L++
Subjt:  FIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSN

Query:  G--KPVLGTQIPAKNSPTSYKDVI--RIFRCLVAKSTPLGPEKNQECSLDKFYGVNAPRNHKVLFL
             +L   I   ++  + K  +  + F  L+ +S PL   +   C+  K   V   ++  ++F+
Subjt:  G--KPVLGTQIPAKNSPTSYKDVI--RIFRCLVAKSTPLGPEKNQECSLDKFYGVNAPRNHKVLFL

Sequences

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CDS sequence

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ATGAGACATGGAGTTGTGAAACAGTTGTTGAAAGGTGGAGTTATCTTCTTGGAGTATGTGAGGTCTGAGAGGAATTTGGCAGATCCTCTCACCAAAGGCCTAACAAGGAG
AGTAATTCTAGAGTCCTCAACAATGGAAGCAAGTGTTCACCCCACACGTGCGTGCTGCCGCCGCAGGTTGGACAGGTGGGTTCGGGTAATCACGCTCGCGCAAAATAAAG
AGAGCGGTTTTGGTGTTAATTCCTCCTATAAAAGGCAGCAAAGCAAGCCTCTCGAGTGCACGAATGAGGATGTAGGTGTTGTATTAACCTTGGGGAGCAACCGGCGTAAA
CGATTTAGCACCGTGGTCGTGCCAAAATTGCTTTCAAGAATCGTACTACGAAATTCCCCGCACCGCCGACTTCATTCACAGCCGGAACTTCACCAGAGTCGCATTGCAGG
TATTTCTTTGAATTCCCAAATACTGGAGTTCCCAGACGAGCTCTTGAAGGATTCAACTAGAGTGGTGAATGCTTTAAGGAGAAGGCTTCGTGCGCTTAATGATACAAACC
AAAGCGATGGCGAAAATGAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGTTGTTGTGTTGATGAGGTTGGAGCAGCTCACGCTAATGCTGATTGTGTCATT
CACTATGGACATACATGTCTGAGCCCTGCCCTTAGAAATCAGCATCCTAAGTTATTGAGTCGTTTCTGCAAATTTGCGCCAGTGTTTGTGCTGTTGATTTTTCTACATTC
GTGTGAGAAACTAAAGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATTCAATTGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTTGTCCAATG
GAAAGCCTGTTTTGGGCACCCAAATACCTGCAAAAAACTCTCCTACATCTTACAAAGATGTGATCCGAATCTTCAGATGTTTGGTTGCAAAAAGCACACCACTTGGTCCT
GAGAAAAATCAAGAATGCTCTTTGGATAAATTCTATGGTGTCAACGCTCCCAGGAACCACAAGGTGCTTTTTCTAGAAAAACCTTGCGAAGAAGCCTTGCTTCTTTCAAT
GAAGCACACTGAGGTGCAAGCCTTAGCAGTTGAATCTGATGTAGCCGATTATATGTTCATTAATTAG

mRNA sequence

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Protein sequence

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MRHGVVKQLLKGGVIFLEYVRSERNLADPLTKGLTRRVILESSTMEASVHPTRACCRRRLDRWVRVITLAQNKESGFGVNSSYKRQQSKPLECTNEDVGVVLTLGSNRRK
RFSTVVVPKLLSRIVLRNSPHRRLHSQPELHQSRIAGISLNSQILEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVI
HYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVLGTQIPAKNSPTSYKDVIRIFRCLVAKSTPLGP
EKNQECSLDKFYGVNAPRNHKVLFLEKPCEEALLLSMKHTEVQALAVESDVADYMFIN

Lag0021830 (gene) of Sponge gourd (AG-4) v1 genome