CuGenDBv2

Gene ID	Lag0025282
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	twinkle homolog protein, chloroplastic/mitochondrial
Genome location	chr10:10820544..10823980
RNA-Seq Expression	Lag0025282
Synteny	Lag0025282
Gene Ontology terms	GO:0006260 - DNA replication (biological process) GO:0006353 - DNA-templated transcription, termination (biological process) GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0032508 - DNA duplex unwinding (biological process) GO:0003690 - double-stranded DNA binding (molecular function) GO:0003697 - single-stranded DNA binding (molecular function) GO:0005524 - ATP binding (molecular function) GO:0043139 - 5'-3' DNA helicase activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7019451.1 Twinkle-like protein, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.3e-52

77.93

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI---SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPP
        MRFLHH+ CLYS FSKLSSLSSSF LMGS  LCKS+SL FLS +   SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRPP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI---SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPP

Query:  AFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
         F+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  AFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

XP_022927084.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata]

1.7e-52

77.4

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP
        MRFLHH+ CLYS FSKLSSLSSSF LMGS  LCKS+SL FLS +    SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP

Query:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        P F+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

XP_022927085.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X2 [Cucurbita moschata]

1.7e-52

77.4

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP
        MRFLHH+ CLYS FSKLSSLSSSF LMGS  LCKS+SL FLS +    SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP

Query:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        P F+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

XP_023000969.1 twinkle homolog protein, chloroplastic/mitochondrial [Cucurbita maxima]

5.0e-52

77.4

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP
        MRFLHH+ CL S FSKLSSLSSSF LMGS  LCKS+SL FLS +    SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP

Query:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        PAF+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

XP_038894379.1 twinkle homolog protein, chloroplastic/mitochondrial [Benincasa hispida]

2.1e-58

83.33

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHISSSSSSQ--RHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA
        MRFLHH+ CLY+PFS LSS SSSFNLMG+IPLCKSTSL  LSH+SSSSSS   ++FLYRSNPLLH  FPV+PMS  KPFSMKPNGVSSFTSH+NVP PPA
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHISSSSSSQ--RHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA

Query:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        FLENPLDEAL ST LNVLRKKL+ELDIDTESCVPGQTNHLLCPM
Subjt:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LYM8 Uncharacterized protein

3.9e-50

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI--SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA
        MRFLH++HCLY+PFSKLSS SS   LMGS PLCKSTSL FLSH+  SSSSSSQ++FLYRS  LLH SFPV+P+S  KPF+MKPNGVSSFTSHANVPRPPA
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI--SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA

Query:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
         LENP D+A  ST+LN+LRKKL++LDID E+CVPGQ   LLCPM
Subjt:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

A0A6J1CLF6 twinkle homolog protein, chloroplastic/mitochondrial isoform X1

2.3e-50

78.47

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHISSSS--SSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA
        MRFLHH+ C +SPF+KLSSLSSSFNLMGSIPLCKSTSL FLSHISSSS  SSQR+FLYR+N LLH SFPVQ MS  K FSMK NGVS FTSHANVP PP 
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHISSSS--SSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA

Query:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
              DE L STQLNVLRKKLEEL+++TESCVPGQTNHLLCPM
Subjt:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

A0A6J1EH06 twinkle homolog protein, chloroplastic/mitochondrial isoform X1

8.3e-53

77.4

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP
        MRFLHH+ CLYS FSKLSSLSSSF LMGS  LCKS+SL FLS +    SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP

Query:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        P F+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

A0A6J1EMW1 twinkle homolog protein, chloroplastic/mitochondrial isoform X2

8.3e-53

77.4

Show/hide

Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP
        MRFLHH+ CLYS FSKLSSLSSSF LMGS  LCKS+SL FLS +    SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP

Query:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        P F+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

A0A6J1KLG2 twinkle homolog protein, chloroplastic/mitochondrial

2.4e-52

77.4

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP
        MRFLHH+ CL S FSKLSSLSSSF LMGS  LCKS+SL FLS +    SSSSSSQR FLY+SN +LH SFPV+PMS GK FSMKPNGVSSFTSHANVPRP
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHI----SSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRP

Query:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM
        PAF+ENPL EAL  T+LN+L+KKL+ELDID E CVPGQTNHLLCPM
Subjt:  PAFLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCPM

SwissProt top hits

e value

%identity

Alignment

B5X582 Twinkle homolog protein, chloroplastic/mitochondrial

1.5e-06

33.57

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Query:  MRFLHHHHCLYSPFSKLSSLSSSFN-LMGSIPLCKSTSL-AFLSHISSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA
        MRFL     L  P      LS S + LMGS    +   L +F S+ SS S S    +   +         +P+S   P+  + NG+SS+ S   VP  P 
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFN-LMGSIPLCKSTSL-AFLSHISSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA

Query:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCP
          E   D+ ++ ++L  LR+KL E  +D E+C PGQ + L+CP
Subjt:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCP

Arabidopsis top hits

e value

%identity

Alignment

AT1G30680.1 toprim domain-containing protein

1.1e-07

33.57

Show/hide

Query:  MRFLHHHHCLYSPFSKLSSLSSSFN-LMGSIPLCKSTSL-AFLSHISSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA
        MRFL     L  P      LS S + LMGS    +   L +F S+ SS S S    +   +         +P+S   P+  + NG+SS+ S   VP  P 
Subjt:  MRFLHHHHCLYSPFSKLSSLSSSFN-LMGSIPLCKSTSL-AFLSHISSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPA

Query:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCP
          E   D+ ++ ++L  LR+KL E  +D E+C PGQ + L+CP
Subjt:  FLENPLDEALISTQLNVLRKKLEELDIDTESCVPGQTNHLLCP

Sequences

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CDS sequence

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ATGCGTTTTCTTCATCACCATCACTGTCTCTATAGCCCCTTCTCCAAGCTCTCTTCCCTTTCCTCCTCATTTAATCTTATGGGTTCTATTCCTTTATGCAAGTCCACTTC
TCTGGCATTCCTGTCACACATTTCTTCTTCTTCCTCCTCACAGAGGCATTTCCTGTACAGAAGCAATCCTCTTCTCCATAGGTCGTTTCCTGTACAACCCATGTCCCATG
GCAAACCCTTTTCAATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCTTTTTGGAGAATCCACTAGACGAGGCTTTAATCTCG
ACTCAATTGAATGTTCTAAGAAAAAAATTGGAGGAACTTGACATTGACACCGAATCTTGTGTTCCAGGGCAGACAAACCACTTGCTTTGTCCAATGGAGCACCTTCGTTC
TTCGGATGTTGCTGCCCTTATTGCTCTTTTGGATGCTACTATTTTCTATCCGGGGAAGCGGGATGATAGGTGTTGGGTTCCTAGTCCTCCAAAAGGATTCTCTTGTGGTT
CCTTTTTCAGCCTTTTGTATAGCAATAGTCCTCTCCCTCCCAATTCTTTGCTTGTTCGTGTTAAAGCCATGGTGAAACTTCTTAAACAGGGTTGGCCTGCATATTTTACT
GTCATTATTGCATTTCTCAATGCAAAGGTGGCGATTCAGGTGAACGGTCCTTCTCCCTATTTATCTCAGAAGATGGATTGTCCTGGCCCTCTCACATTTTCTGGATGGAA
ATGTGGCTCAAGTGCTTTGTGGCCATTCTTTCAAGAGGATAGCAAAGCCTCAAGTTGA

mRNA sequence

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Protein sequence

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MRFLHHHHCLYSPFSKLSSLSSSFNLMGSIPLCKSTSLAFLSHISSSSSSQRHFLYRSNPLLHRSFPVQPMSHGKPFSMKPNGVSSFTSHANVPRPPAFLENPLDEALIS
TQLNVLRKKLEELDIDTESCVPGQTNHLLCPMEHLRSSDVAALIALLDATIFYPGKRDDRCWVPSPPKGFSCGSFFSLLYSNSPLPPNSLLVRVKAMVKLLKQGWPAYFT
VIIAFLNAKVAIQVNGPSPYLSQKMDCPGPLTFSGWKCGSSALWPFFQEDSKASS

Lag0025282 (gene) of Sponge gourd (AG-4) v1 genome