CuGenDBv2

Gene ID	Lag0025289
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	twinkle homolog protein, chloroplastic/mitochondrial
Genome location	chr10:10892943..10895830
RNA-Seq Expression	Lag0025289
Synteny	Lag0025289
Gene Ontology terms	GO:0006260 - DNA replication (biological process) GO:0032508 - DNA duplex unwinding (biological process) GO:0003697 - single-stranded DNA binding (molecular function) GO:0005524 - ATP binding (molecular function) GO:0043139 - 5'-3' DNA helicase activity (molecular function)
InterPro domains	IPR006171 - TOPRIM domain IPR027032 - Twinkle-like protein IPR034154 - Archaeal primase DnaG/twinkle, TOPRIM domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7019451.1 Twinkle-like protein, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma]

8.7e-73

79.55

Show/hide

Query:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS
        ++++     I+IAFTY RRGALISCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPS SQK          
Subjt:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS

Query:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
              DTKYQYLWNCKDYLSKASRII+ATDGD PGQALAEEIARRVGRERCWRVKWPKKNE +HFKDANE+L  L
Subjt:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

XP_022142394.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Momordica charantia]

6.6e-73

83.23

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        IAIAFTY+R G L+SCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGE+DKLSM EAGFHNCVSVPDGAPPSVSQK                DTK
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
        YQYLWNCK+YLSKASRII+ATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANE+L  L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

XP_022142395.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X2 [Momordica charantia]

6.6e-73

83.23

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        IAIAFTY+R G L+SCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGE+DKLSM EAGFHNCVSVPDGAPPSVSQK                DTK
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
        YQYLWNCK+YLSKASRII+ATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANE+L  L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

XP_022927084.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata]

8.7e-73

79.55

Show/hide

Query:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS
        ++++     I+IAFTY RRGALISCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPS SQK          
Subjt:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS

Query:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
              DTKYQYLWNCKDYLSKASRII+ATDGD PGQALAEEIARRVGRERCWRVKWPKKNE +HFKDANE+L  L
Subjt:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

XP_023000969.1 twinkle homolog protein, chloroplastic/mitochondrial [Cucurbita maxima]

1.0e-73

80.11

Show/hide

Query:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS
        ++++     I+IAFTY RRGALISCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPS SQK          
Subjt:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS

Query:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
              DTKYQYLWNCKDYLSKASRII+ATDGDPPGQALAEEIARRVGRERCWRVKWPKKNE +HFKDANE+L  L
Subjt:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

TrEMBL top hits

e value

%identity

Alignment

A0A6J1CKU2 twinkle homolog protein, chloroplastic/mitochondrial isoform X2

3.2e-73

83.23

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        IAIAFTY+R G L+SCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGE+DKLSM EAGFHNCVSVPDGAPPSVSQK                DTK
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
        YQYLWNCK+YLSKASRII+ATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANE+L  L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

A0A6J1CLF6 twinkle homolog protein, chloroplastic/mitochondrial isoform X1

3.2e-73

83.23

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        IAIAFTY+R G L+SCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGE+DKLSM EAGFHNCVSVPDGAPPSVSQK                DTK
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
        YQYLWNCK+YLSKASRII+ATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANE+L  L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

A0A6J1EH06 twinkle homolog protein, chloroplastic/mitochondrial isoform X1

4.2e-73

79.55

Show/hide

Query:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS
        ++++     I+IAFTY RRGALISCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPS SQK          
Subjt:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS

Query:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
              DTKYQYLWNCKDYLSKASRII+ATDGD PGQALAEEIARRVGRERCWRVKWPKKNE +HFKDANE+L  L
Subjt:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

A0A6J1EMW1 twinkle homolog protein, chloroplastic/mitochondrial isoform X2

4.2e-73

79.55

Show/hide

Query:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS
        ++++     I+IAFTY RRGALISCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPS SQK          
Subjt:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS

Query:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
              DTKYQYLWNCKDYLSKASRII+ATDGD PGQALAEEIARRVGRERCWRVKWPKKNE +HFKDANE+L  L
Subjt:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

A0A6J1KLG2 twinkle homolog protein, chloroplastic/mitochondrial

5.0e-74

80.11

Show/hide

Query:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS
        ++++     I+IAFTY RRGALISCKYRDVNKKFWQEANTEKIFYGLD IDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPS SQK          
Subjt:  MLERLKTLPIAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFS

Query:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL
              DTKYQYLWNCKDYLSKASRII+ATDGDPPGQALAEEIARRVGRERCWRVKWPKKNE +HFKDANE+L  L
Subjt:  HFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEILNLL

SwissProt top hits

e value

%identity

Alignment

B5X582 Twinkle homolog protein, chloroplastic/mitochondrial

7.4e-59

67.07

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        I IAFTY++RG L+SCKYR + K F+QE  T +I YGLD I+  S++IIVEGEIDKL+M EAGF NCVSVPDGAP  VS K    +I S        DTK
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL
        Y++LWNC DYL KASRI+IATDGD PGQA+AEEIARR+G+ERCWRVKWPKK+E +HFKDANE+L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL

F4I6E6 Primase homolog protein

1.7e-58

67.68

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        I IAFTY++RG L+SCKYR + KKF QE NT KI YGLD I+  S+IIIVEGE DKL+M EAGF NCVSVPDGAP +VS K    +I S S      DT 
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL
        ++Y+WNC DYL KASRI+IATDGD PGQALAEE+ARR+G+ERCW VKWPKK+E +HFKDANE+L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL

Arabidopsis top hits

e value

%identity

Alignment

AT1G30660.1 nucleic acid binding;nucleic acid binding

1.2e-59

67.68

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        I IAFTY++RG L+SCKYR + KKF QE NT KI YGLD I+  S+IIIVEGE DKL+M EAGF NCVSVPDGAP +VS K    +I S S      DT 
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL
        ++Y+WNC DYL KASRI+IATDGD PGQALAEE+ARR+G+ERCW VKWPKK+E +HFKDANE+L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL

AT1G30680.1 toprim domain-containing protein

5.3e-60

67.07

Show/hide

Query:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK
        I IAFTY++RG L+SCKYR + K F+QE  T +I YGLD I+  S++IIVEGEIDKL+M EAGF NCVSVPDGAP  VS K    +I S        DTK
Subjt:  IAIAFTYYRRGALISCKYRDVNKKFWQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTK

Query:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL
        Y++LWNC DYL KASRI+IATDGD PGQA+AEEIARR+G+ERCWRVKWPKK+E +HFKDANE+L
Subjt:  YQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIARRVGRERCWRVKWPKKNEVDHFKDANEIL

Sequences

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CDS sequence

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ATGTGTCTGAAAGCATCGGAAAACTTAGACCACCTTTTTCTGCATTGTGAGTTCGTCGGAAAAGCTTGGAATTTTGTTGCAAAGTTGCTGGGAATCTTGCTGTGCTTGCC
AAAGAAGATTGACGATTGGCTTTGTGAAGGCTTGATGGCGTGGAATTTCAAAAGGAAAGCTAGGATTATGGCTAGTTGTGTGTTTATGGCTACCCTTTGGCTTTTGTGGG
TGAAAGAAATGCTAGAACGTTTGAAGACACTTCCGATTGCTATTGCATTTACATATTATCGACGTGGAGCATTGATTAGTTGCAAGTATCGTGATGTCAACAAAAAGTTC
TGGCAGGAGGCAAATACTGAGAAAATATTTTATGGATTGGATTACATAGATGGCGCAAGTGATATCATCATTGTTGAAGGGGAGATAGACAAGCTTTCAATGGCAGAAGC
TGGTTTCCATAATTGCGTGAGTGTTCCAGATGGTGCACCACCATCAGTTTCCCAAAAGACGTACCTCCTACAGATCAGCTCTTTCTCCCATTTTACAGCTGCATGGGATA
CGAAGTATCAGTATTTATGGAACTGCAAAGATTACTTGAGTAAGGCATCACGCATTATCATTGCTACTGATGGTGATCCTCCTGGTCAAGCTTTAGCAGAGGAGATTGCA
CGTCGTGTTGGAAGGGAAAGATGTTGGAGGGTCAAATGGCCAAAAAAAAATGAGGTCGACCATTTCAAAGATGCAAATGAGATCTTGAACTTGCTTTGGGCAATGGACGT
TATTGATGAGATAAGGATCAGGAGTGGAATGTCATCTTTCTGGTGCTAA

mRNA sequence

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Protein sequence

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MCLKASENLDHLFLHCEFVGKAWNFVAKLLGILLCLPKKIDDWLCEGLMAWNFKRKARIMASCVFMATLWLLWVKEMLERLKTLPIAIAFTYYRRGALISCKYRDVNKKF
WQEANTEKIFYGLDYIDGASDIIIVEGEIDKLSMAEAGFHNCVSVPDGAPPSVSQKTYLLQISSFSHFTAAWDTKYQYLWNCKDYLSKASRIIIATDGDPPGQALAEEIA
RRVGRERCWRVKWPKKNEVDHFKDANEILNLLWAMDVIDEIRIRSGMSSFWC

Lag0025289 (gene) of Sponge gourd (AG-4) v1 genome