CuGenDBv2

Gene ID	Lag0025415
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	LINE-1 reverse transcriptase-like
Genome location	chr10:12665338..12665652
RNA-Seq Expression	Lag0025415
Synteny	Lag0025415
Gene Ontology terms	GO:0006796 - phosphate-containing compound metabolic process (biological process) GO:0044260 - cellular macromolecule metabolic process (biological process) GO:0090304 - nucleic acid metabolic process (biological process) GO:0016020 - membrane (cellular component) GO:0016772 - transferase activity, transferring phosphorus-containing groups (molecular function) GO:0016787 - hydrolase activity (molecular function) GO:0097159 - organic cyclic compound binding (molecular function) GO:0140640 - catalytic activity, acting on a nucleic acid (molecular function) GO:0140657 - ATP-dependent activity (molecular function) GO:1901363 - heterocyclic compound binding (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

RVW88105.1 putative ribonuclease H protein [Vitis vinifera]

1.2e-22

44.23

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC+V + L+ YLG P G  +  L++WD + ++   KL +W+   LSKGGR+TL +S L S+PIY  S+ + PK+V+K++EK+ RDF+W GGS     ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        +NWE
Subjt:  VNWE

RVX22948.1 ATP-dependent RNA helicase DBP2 [Vitis vinifera]

3.1e-23

45.19

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC+V +F + YLG P G +H  L+ WD + ++   +L +W+   LSKGGR+TL +S L S+PIY  SL + PK V+K++EK+ RDF+W GGS +   ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        +NWE
Subjt:  VNWE

RVX23975.1 LINE-1 reverse transcriptase-like [Vitis vinifera]

2.0e-22

43.27

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC++ +F +NYLG P G  H  L +WD + ++   +L +W+   LSKGGR+TL +S L S+PIY  S+ + PK+V+K++EK+ RDF+W GGS     ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        +NW+
Subjt:  VNWE

XP_010653273.1 PREDICTED: uncharacterized protein LOC104879995 [Vitis vinifera]

9.2e-23

44.23

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GCKV +F + YLG P G +H  L+ WD + ++   +L +W+   LSKGGR+TL +S L S+PIY  S+ + PK V+K++EK+ RDF+W GGS +   ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        +NW+
Subjt:  VNWE

XP_021991187.1 uncharacterized protein LOC110887939 [Helianthus annuus]

1.6e-22

48.08

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GCK E F   YLG P G N +R+++W+ ++D F A+L +W+  +LS GGRVTL +S+L SLPIY+FSL K P  VIK++E I+R F+W G      +  
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        V+WE
Subjt:  VNWE

TrEMBL top hits

e value

%identity

Alignment

A0A438KP17 ATP-dependent RNA helicase DBP2

1.5e-23

45.19

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC+V +F + YLG P G +H  L+ WD + ++   +L +W+   LSKGGR+TL +S L S+PIY  SL + PK V+K++EK+ RDF+W GGS +   ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        +NWE
Subjt:  VNWE

A0A803P465 Uncharacterized protein

2.6e-23

46.15

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC+V ++ + YLG P GG+  + + W+P++DK   +LD W+   LSKGGR+TL QS+L+SLP+Y  SL KAP++V K +EK++RDF+W G     G ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        V W+
Subjt:  VNWE

A0A803P8A0 Uncharacterized protein

6.8e-24

47.12

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC+V  + + YLG P GG+  +   W+P+LDK   ++D W+   LS+GGR+TL QS+L+SLPIY+ SL K PK V+K++EK++RDF W GG    G ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        V W+
Subjt:  VNWE

A0A803PZS1 Uncharacterized protein

1.4e-24

50.96

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GCKV  + + YLG P GG+  +   W+P+LDK   +LD W+   LS+GGR+TL QS+L+SLPIY+ SL KAPK VIK +EK++RDF W GG    G ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        V W+
Subjt:  VNWE

A0A803QQM3 Uncharacterized protein

2.4e-24

48.08

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Query:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV
        +GC+V  + + YLG P GG+  + + W+P+LDK   ++D W+   LS+GGR+TL QS+L+SLPIY  SL KAPK V+K++EK++RDF W GG    G ++
Subjt:  MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNV

Query:  VNWE
        V W+
Subjt:  VNWE

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

1.0e-08

27.03

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Query:  LLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNVVNW
        +L++ ++++  WR   LS  GR+TL +++L+S+P++  S +  P++++ +++++ R F+W   + K   ++V W
Subjt:  LLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNVVNW

Arabidopsis top hits

e value

%identity

Alignment

AT3G24255.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

1.6e-09

29.79

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Query:  INYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNVVNW
        + YLG P        + + PL++K   ++  W    LS  GR+ L  S+++SL  +  S  + P A IK+++ I   F+WSG         V W
Subjt:  INYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNVVNW

Sequences

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CDS sequence

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ATGGGCTGCAAAGTTGAGAACTTCCTAATTAACTATCTTGGATTTCCCCCGGGAGGGAATCATCATAGGCTCGCGTCCTGGGACCCTTTGTTGGATAAGTTCAATGCTAA
ATTGGATGTGTGGAGGCACTTTCTTTTATCCAAGGGTGGTAGAGTTACATTGGCTCAATCGATCCTCAATAGCCTCCCAATTTACCACTTTTCTTTGCTTAAAGCTCCAA
AGGCGGTGATAAAGAAGATGGAAAAGATCATTAGAGATTTTGTCTGGAGTGGTGGATCCTACAAACCAGGCAGCAACGTTGTTAATTGGGAGTAG

mRNA sequence

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Protein sequence

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MGCKVENFLINYLGFPPGGNHHRLASWDPLLDKFNAKLDVWRHFLLSKGGRVTLAQSILNSLPIYHFSLLKAPKAVIKKMEKIIRDFVWSGGSYKPGSNVVNWE

Lag0025415 (gene) of Sponge gourd (AG-4) v1 genome