CuGenDBv2

Gene ID	Lag0027451
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	RNase H domain-containing protein
Genome location	chr8:960031..965262
RNA-Seq Expression	Lag0027451
Synteny	Lag0027451
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

PRQ34202.1 putative ribonuclease H-like domain-containing protein [Rosa chinensis]

5.6e-08

32.2

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Query:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA
        G + GAM + +   LSP   E  A++HGLR  A +   R+ +  D+L+ I  +  E  +     H + E+  L + F      ++ R+ N+ A  LAK+A
Subjt:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA

Query:  LMHQQTMLWLENFPLWLL
        L   Q  L LE+ P WL+
Subjt:  LMHQQTMLWLENFPLWLL

XP_022158377.1 uncharacterized protein LOC111024874 [Momordica charantia]

2.7e-10

36.67

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Query:  NICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL
        ++  A  I     LSP  AE++ I+ GL+  A      + V SDSL AI++I  EI    + ++W+ EI  L   F  + FS+  R  NR A  LAK  +
Subjt:  NICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL

Query:  MH-QQTMLWLENFPLWLLSM
             T  WL NFP WLL +
Subjt:  MH-QQTMLWLENFPLWLLSM

XP_022158489.1 uncharacterized protein LOC111024968 [Momordica charantia]

5.6e-08

30.47

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Query:  ICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWL-EVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL
        I GAM +      +P  A++ AI  GL L  R+ + RV V +DSL A+ +I G+   W  E   W+ +I    + F  +CF ++ R+ N  A  L ++ +
Subjt:  ICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWL-EVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL

Query:  MHQQTMLWLENFPLWLLSMGNQSHDKSI
          +   LW  +FP+WL  +       S+
Subjt:  MHQQTMLWLENFPLWLLSMGNQSHDKSI

XP_024164210.1 uncharacterized protein LOC112171231 [Rosa chinensis]

1.5e-08

31.62

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Query:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA
        GN+ GAM + +   ++P   E  A++HGLR    +   R+ V  D+L+ I  +     ++    H I E+  L + F      ++ R+ N+ A  LAK+A
Subjt:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA

Query:  LMHQQTMLWLENFPLWL
        L   Q  L LE+ P WL
Subjt:  LMHQQTMLWLENFPLWL

XP_024177947.1 uncharacterized protein LOC112183863 [Rosa chinensis]

2.5e-08

31.62

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Query:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA
        GN+ GAM + +   ++P   E  A++HGLR    +   R+ V  D L+ I  +     ++    H I E+  L + F      ++ R+ N+ A  LAK+A
Subjt:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA

Query:  LMHQQTMLWLENFPLWL
        L   Q  L LE+ P WL
Subjt:  LMHQQTMLWLENFPLWL

TrEMBL top hits

e value

%identity

Alignment

A0A251PKK0 RNase H domain-containing protein

6.7e-07

31.01

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Query:  LVVNEVGNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGE-WLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKA
        +V N+ G +  AM +  +SA S   AE+ A + G++         + + SDS   +  +  +  E W    H I +I    + F  V  SY PR  N+ A
Subjt:  LVVNEVGNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGE-WLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKA

Query:  DALAKDALMHQQTMLWLENFPLWLLSMGN
          LAK AL     + W+E  P WL S+ N
Subjt:  DALAKDALMHQQTMLWLENFPLWLLSMGN

A0A2P6QJ46 Putative ribonuclease H-like domain-containing protein

2.7e-08

32.2

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Query:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA
        G + GAM + +   LSP   E  A++HGLR  A +   R+ +  D+L+ I  +  E  +     H + E+  L + F      ++ R+ N+ A  LAK+A
Subjt:  GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDA

Query:  LMHQQTMLWLENFPLWLL
        L   Q  L LE+ P WL+
Subjt:  LMHQQTMLWLENFPLWLL

A0A6J1DX30 uncharacterized protein LOC111024874

1.3e-10

36.67

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Query:  NICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL
        ++  A  I     LSP  AE++ I+ GL+  A      + V SDSL AI++I  EI    + ++W+ EI  L   F  + FS+  R  NR A  LAK  +
Subjt:  NICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL

Query:  MH-QQTMLWLENFPLWLLSM
             T  WL NFP WLL +
Subjt:  MH-QQTMLWLENFPLWLLSM

A0A6J1DZK3 uncharacterized protein LOC111024968

2.7e-08

30.47

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Query:  ICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWL-EVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL
        I GAM +      +P  A++ AI  GL L  R+ + RV V +DSL A+ +I G+   W  E   W+ +I    + F  +CF ++ R+ N  A  L ++ +
Subjt:  ICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWL-EVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDAL

Query:  MHQQTMLWLENFPLWLLSMGNQSHDKSI
          +   LW  +FP+WL  +       S+
Subjt:  MHQQTMLWLENFPLWLLSMGNQSHDKSI

A0A803NTU1 Uncharacterized protein

8.8e-07

34.09

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Query:  YNQPSPPNSPLVVNEV-GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFS
        YN     +S +V+ +  G +  +     S  LS  PAE  AI+ GL+L     +++  V SD LNAI +IN       +V + + EI  L   F LV F 
Subjt:  YNQPSPPNSPLVVNEV-GNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFS

Query:  YIPRDKNRKADALAKDALMHQQTMLWLENFPL
        +  RD N  A +LAK AL  + +  W    PL
Subjt:  YIPRDKNRKADALAKDALMHQQTMLWLENFPL

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G27870.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

3.1e-04

30.21

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Query:  AEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDALMHQQTML---WLEN
        +E+QA++  ++       +RV    D+     +ING    +  V +WI +IH   + F  + F++I R  N  AD LAK  L +Q   L   W+ N
Subjt:  AEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDALMHQQTML---WLEN

AT4G09490.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.6e-05

36.05

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Query:  PFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDALM
        P  AE  A+   L+    + I ++ + SDS   I  I  E     E    I +I +L  GF  V FS++PR +NR AD LAK +L+
Subjt:  PFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDALM

Sequences

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CDS sequence

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ATGTCACTCATTGACTTTTCTACCGATGACCGACCGACGACTTTTCCAGCGACCGCCGTCGACTTTCCGGCCACTTTTCCGGCAATCACCGACGACTTTTCTGCTATTAT
AACCCCTAGCTATTATAACCAACCCAGCCCCCCAAACAGCCCTTTAGTGGTTAATGAAGTCGGCAATATTTGTGGAGCAATGGAGATACATGAGAGTTCAGCTTTGTCAC
CGTTCCCAGCGGAAGTCCAGGCTATAATGCATGGACTAAGATTGACTGCACGCATGGAAATACAAAGAGTTCATGTGTACTCAGACTCTTTAAATGCGATTCGGATGATC
AATGGAGAGATTGGCGAGTGGCTGGAAGTTCGGCACTGGATATCAGAAATTCATGATCTATGCAAAGGGTTCCTTTTAGTGTGTTTCTCTTATATCCCAAGGGATAAAAA
TAGGAAAGCAGATGCTCTAGCGAAGGATGCGTTAATGCACCAGCAAACTATGTTGTGGTTAGAAAATTTTCCGCTATGGCTTTTATCAATGGGTAACCAATCCCACGATA
AGAGTATTGGTCGAATCGGGGTGAATCGGGACACCGTGAGTCGGCTGGGGGAGAACAAGCTTGACTTACCCAAGTGGGTCGAGGGTCGACCTCGCCCTTGGGTCGAGGCC
GAGCATGGGATTGGGCCAAGGCCCGATCCCTCCAGCCTTGATCCAGTTGCTCCGACCTATTCCTCCTTCAGTGCCCACTTCTTGATCGTATCTCCGGATCGATGTTGTGT
TGTCCTCGTCGATCCCTTACATTTTGGACCAGCTTAA

mRNA sequence

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Protein sequence

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MSLIDFSTDDRPTTFPATAVDFPATFPAITDDFSAIITPSYYNQPSPPNSPLVVNEVGNICGAMEIHESSALSPFPAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMI
NGEIGEWLEVRHWISEIHDLCKGFLLVCFSYIPRDKNRKADALAKDALMHQQTMLWLENFPLWLLSMGNQSHDKSIGRIGVNRDTVSRLGENKLDLPKWVEGRPRPWVEA
EHGIGPRPDPSSLDPVAPTYSSFSAHFLIVSPDRCCVVLVDPLHFGPA

Lag0027451 (gene) of Sponge gourd (AG-4) v1 genome