CuGenDBv2

Gene ID	Lag0028940
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr8:33203282..33203659
RNA-Seq Expression	Lag0028940
Synteny	Lag0028940
Gene Ontology terms	GO:0016020 - membrane (cellular component) GO:0005488 - binding (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

CAB4263564.1 unnamed protein product [Prunus armeniaca]

1.6e-34

56.8

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        MC+ K  G +GFR +  FNQALL KQ WR+++ P SL+  + K RYFPHSDF  AS G  PSF W+SLLWGR LL  G RWRIG+GR + IYG  WVP +
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
            +QS  +LP++SRVCDLF+ SG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

ONI01138.1 hypothetical protein PRUPE_6G123900 [Prunus persica]

2.1e-34

53.6

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        +C+ K  G LGFR++E FNQALLAKQCWR+++ PESL+  + + RY P   F  A +G  PSFIWRSL WG+ LLN+G RWR+G+G SI +Y   W+P  
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
           ++ S   LPLS+RVCDLF+ SG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

XP_008237273.1 PREDICTED: uncharacterized protein LOC103336015 [Prunus mume]

3.6e-34

57.6

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        MC+ K  G LGFR +  FNQALL KQ WR+++ P SL+  +LK RYFP+SDF  AS G  PSF W+SLLWGR LL  G RWRIG+GR + IYG  WVP +
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
            +QS  +LP +SRVCDLF+ SG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

XP_022150918.1 uncharacterized protein LOC111018954 [Momordica charantia]

1.9e-35

58.97

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Query:  PKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL
        PKC G +GFR++E FN+ALLAKQCWR++  P S+L  VLKGRYF    F  A +   PS+IWRS+LWGR LL +G RWRIGNG S+ IYG NWVP+ P+L
Subjt:  PKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL

Query:  RVQSASSLPLSSRVCDL
        ++ S+  LPL SRV  L
Subjt:  RVQSASSLPLSSRVCDL

XP_022159057.1 uncharacterized protein LOC111025497 [Momordica charantia]

5.6e-35

60.8

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        MC  KCMG LGFR++EFFNQALLAKQ W++  DP SLL  VLK +YF + DF  AS+G R SFIWRSLLWG  LL  G RWR+GNG +I IY  NW+P +
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
          L VQS  SLP  S V D ++PSG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

TrEMBL top hits

e value

%identity

Alignment

A0A251NPF0 Reverse transcriptase domain-containing protein

1.0e-34

53.6

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        +C+ K  G LGFR++E FNQALLAKQCWR+++ PESL+  + + RY P   F  A +G  PSFIWRSL WG+ LLN+G RWR+G+G SI +Y   W+P  
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
           ++ S   LPLS+RVCDLF+ SG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

A0A6J1DAR4 uncharacterized protein LOC111018954

9.4e-36

58.97

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Query:  PKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL
        PKC G +GFR++E FN+ALLAKQCWR++  P S+L  VLKGRYF    F  A +   PS+IWRS+LWGR LL +G RWRIGNG S+ IYG NWVP+ P+L
Subjt:  PKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL

Query:  RVQSASSLPLSSRVCDL
        ++ S+  LPL SRV  L
Subjt:  RVQSASSLPLSSRVCDL

A0A6J1DYS7 uncharacterized protein LOC111025497

2.7e-35

60.8

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        MC  KCMG LGFR++EFFNQALLAKQ W++  DP SLL  VLK +YF + DF  AS+G R SFIWRSLLWG  LL  G RWR+GNG +I IY  NW+P +
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
          L VQS  SLP  S V D ++PSG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

A0A6J5TIF9 Reverse transcriptase domain-containing protein

7.9e-35

56.8

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        MC+ K  G +GFR +  FNQALL KQ WR+++ P SL+  + K RYFPHSDF  AS G  PSF W+SLLWGR LL  G RWRIG+GR + IYG  WVP +
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
            +QS  +LP++SRVCDLF+ SG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

M5W5F3 Reverse transcriptase domain-containing protein (Fragment)

1.0e-34

53.6

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN
        +C+ K  G LGFR++E FNQALLAKQCWR+++ PESL+  + + RY P   F  A +G  PSFIWRSL WG+ LLN+G RWR+G+G SI +Y   W+P  
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDN

Query:  PSLRVQSASSLPLSSRVCDLFSPSG
           ++ S   LPLS+RVCDLF+ SG
Subjt:  PSLRVQSASSLPLSSRVCDLFSPSG

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

5.5e-09

32.43

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Query:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFP---HSDFWGASLGHRPSFIWRSLLWG-RALLNQGCRWRIGNGRSIPIYGSNW
        +C PK  G LG R  +  N+AL++K  WR++Q+  SL  +VL+ +Y         W    G   S  WRS+  G R +++ G  W  G+G+ I  +   W
Subjt:  MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFP---HSDFWGASLGHRPSFIWRSLLWG-RALLNQGCRWRIGNGRSIPIYGSNW

Query:  VPDNPSLRVQS
        V   P L + +
Subjt:  VPDNPSLRVQS

P93295 Uncharacterized mitochondrial protein AtMg00310

1.4e-20

46.88

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Query:  GSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL
        G LGFR++ +FNQALLAKQ +R+I  P +LL  +L+ RYFPHS     S+G RPS+ WRS++ GR LL++G    IG+G    ++   W+ D   L
Subjt:  GSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL

Arabidopsis top hits

e value

%identity

Alignment

AT4G29090.1 Ribonuclease H-like superfamily protein

3.4e-22

41.86

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Query:  KCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNP---
        K  G +GF+++E FN ALL KQ WR++  PESL+  V K RYF  SD   A LG RPSF+W+S+   + +L QG R  +GNG  I I+   W+   P   
Subjt:  KCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNP---

Query:  SLRVQSA-----SSLPLSSRVCDLFSPSG
        +LR+Q       +S+    +V DL   SG
Subjt:  SLRVQSA-----SSLPLSSRVCDLFSPSG

ATMG00310.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

9.9e-22

46.88

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Query:  GSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL
        G LGFR++ +FNQALLAKQ +R+I  P +LL  +L+ RYFPHS     S+G RPS+ WRS++ GR LL++G    IG+G    ++   W+ D   L
Subjt:  GSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSL

Sequences

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CDS sequence

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ATGTGTCGTCCTAAGTGTATGGGGAGTTTGGGTTTCCGCAATATGGAGTTTTTTAATCAAGCGTTGTTGGCTAAGCAGTGCTGGCGAGTAATCCAAGATCCTGAGTCCCT
CCTGGGTGTCGTCCTTAAGGGTAGGTATTTTCCCCATTCTGATTTCTGGGGGGCGTCTTTGGGCCATCGGCCATCTTTCATCTGGCGTAGTTTGTTGTGGGGCCGGGCAC
TGCTGAACCAAGGGTGCAGATGGAGGATTGGTAATGGTCGGTCTATTCCCATATATGGTTCGAATTGGGTGCCGGATAATCCATCTTTGCGTGTGCAGTCTGCTTCTTCA
CTTCCTTTATCTAGTAGGGTCTGTGATCTGTTTTCTCCGTCAGGTTAG

mRNA sequence

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Protein sequence

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MCRPKCMGSLGFRNMEFFNQALLAKQCWRVIQDPESLLGVVLKGRYFPHSDFWGASLGHRPSFIWRSLLWGRALLNQGCRWRIGNGRSIPIYGSNWVPDNPSLRVQSASS
LPLSSRVCDLFSPSG

Lag0028940 (gene) of Sponge gourd (AG-4) v1 genome