CuGenDBv2

Gene ID	Lag0029361
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	DNA polymerase epsilon catalytic subunit
Genome location	chr8:38173204..38174811
RNA-Seq Expression	Lag0029361
Synteny	Lag0029361
Gene Ontology terms	GO:0000278 - mitotic cell cycle (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0071897 - DNA biosynthetic process (biological process) GO:0006272 - leading strand elongation (biological process) GO:0006287 - base-excision repair, gap-filling (biological process) GO:0006297 - nucleotide-excision repair, DNA gap filling (biological process) GO:0045004 - DNA replication proofreading (biological process) GO:0008622 - epsilon DNA polymerase complex (cellular component) GO:0008310 - single-stranded DNA 3'-5' exodeoxyribonuclease activity (molecular function) GO:0000166 - nucleotide binding (molecular function) GO:0008270 - zinc ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function) GO:0003887 - DNA-directed DNA polymerase activity (molecular function) GO:0003677 - DNA binding (molecular function)
InterPro domains	IPR006133 - DNA-directed DNA polymerase, family B, exonuclease domain IPR012337 - Ribonuclease H-like superfamily IPR029703 - DNA polymerase epsilon catalytic subunit IPR036397 - Ribonuclease H superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAB5561539.1 hypothetical protein DKX38_006496 [Salix brachista]

1.9e-16

91.3

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

KAF9684575.1 hypothetical protein SADUNF_Sadunf04G0132300 [Salix dunnii]

1.9e-16

91.3

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

KAG5248027.1 DNA-directed DNA polymerase epsilon catalytic family protein [Salix suchowensis]

1.9e-16

91.3

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

XP_022153061.1 DNA polymerase epsilon catalytic subunit A-like isoform X1 [Momordica charantia]

8.7e-17

93.48

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        +LELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

XP_022153062.1 DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia]

8.7e-17

93.48

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        +LELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

TrEMBL top hits

e value

%identity

Alignment

A0A5N5N2H6 DNA polymerase epsilon catalytic subunit

9.4e-17

91.3

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

A0A6J1DHX7 DNA polymerase epsilon catalytic subunit

4.2e-17

93.48

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        +LELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

A0A6J1DJK0 DNA polymerase epsilon catalytic subunit

4.2e-17

93.48

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        +LELL LWF HMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

A0A6J1H1A3 DNA polymerase epsilon catalytic subunit

2.1e-16

89.13

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        +LELL  WF HMREVKPGIYVTYNGDYFDWPF+ESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

A0A6J1K6J2 DNA polymerase epsilon catalytic subunit

2.1e-16

89.13

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        +LELL  WF HMREVKPGIYVTYNGDYFDWPF+ESRAAYHGFKMSD
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

SwissProt top hits

e value

%identity

Alignment

F4HW04 DNA polymerase epsilon catalytic subunit A

5.5e-14

67.39

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL  WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG KM++
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

F4IFN6 DNA polymerase epsilon catalytic subunit B

7.2e-14

67.39

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL  WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG KM++
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

P87154 DNA polymerase epsilon catalytic subunit A

9.1e-09

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ LL  +F H+R  KP + VTYNGD+FDWPF+++RAA+HG  +++
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

Q07864 DNA polymerase epsilon catalytic subunit A

1.1e-09

59.09

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKM
        +  L+  WF H++E KP I VTYNGD+FDWPF+E+RAA HG  M
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKM

Q9WVF7 DNA polymerase epsilon catalytic subunit A

6.3e-10

59.09

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKM
        ++ L+  WF H++E KP I VTYNGD+FDWPF+E+RAA HG  M
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKM

Arabidopsis top hits

e value

%identity

Alignment

AT1G08260.1 DNA polymerase epsilon catalytic subunit

3.9e-15

67.39

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL  WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG KM++
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

AT2G27120.1 DNA polymerase epsilon catalytic subunit

5.1e-15

67.39

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Query:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD
        ++ELL  WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG KM++
Subjt:  QLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD

Sequences

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CDS sequence

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ATGAGGCCTCGAGAAAGAGGTCGCCCACCCTCGTCTGTTTCGCCCCCTGCGCACCAAATCTCCCGTGCCGACGGTTCTCCTTCGTCGTCGTCGTCGTTTATCTCTCGCCG
CCGTCGTTGTTTTGCTCGTGCTTTCGCCGTCGTTCCACTCCGTAGCCATCGTCTTTTCTCGCTTCAACAGTTAGAGCTTCTCATACTGTGGTTTGTCCATATGCGAGAGG
TGAAACCTGGTATCTACGTCACCTACAATGGCGACTATTTTGACTGGCCATTCCTTGAAAGCAGAGCAGCATATCATGGATTTAAAATGAGTGATGCAAAACTTGAGCAA
TCAGAAGCAAAGATTATCCAGGTGGAATCAATGGTTGAGGAAGAGAGGAGTGCAAGACTTGAAACGAAAGCTGAGCTAGAAAACTTGAGAGGAATGATCCAACAATTCAT
GTCGTCACAAGGAGGAGAAACATCGAAATATGTATGA

mRNA sequence

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Protein sequence

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MRPRERGRPPSSVSPPAHQISRADGSPSSSSSFISRRRRCFARAFAVVPLRSHRLFSLQQLELLILWFVHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSDAKLEQ
SEAKIIQVESMVEEERSARLETKAELENLRGMIQQFMSSQGGETSKYV

Lag0029361 (gene) of Sponge gourd (AG-4) v1 genome