CuGenDBv2

Gene ID	Lag0031583
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr11:10362248..10362622
RNA-Seq Expression	Lag0031583
Synteny	Lag0031583
Gene Ontology terms	GO:0006139 - nucleobase-containing compound metabolic process (biological process) GO:0016301 - kinase activity (molecular function)
InterPro domains	IPR026960 - Reverse transcriptase zinc-binding domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAF4399926.1 hypothetical protein G4B88_021140 [Cannabis sativa]

8.4e-15

36.07

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Query:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSG-SNFAERI
        +S+  S +  WW++ WK K+PPK ++F+WK +H  L     L++R +  SP C  C S +ETI+H L  C R+K +      R+  N P+ G S+ A+ +
Subjt:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSG-SNFAERI

Query:  SWLSQNLGDEDFEKACITFWAI
          LS  L   DFE   +  W+I
Subjt:  SWLSQNLGDEDFEKACITFWAI

XP_030483669.1 uncharacterized protein LOC115700241 [Cannabis sativa]

7.8e-13

29.75

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Query:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS
        E+++ + L  WWT  WK K+P K ++F+WK +H+ LP    L ++ +  +P C  C+  +ET++H L  C R+K +        +  + +S ++ A+ + 
Subjt:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS

Query:  WLSQNLGDEDFEKACITFWAI
        ++S N    +FEK  +  W+I
Subjt:  WLSQNLGDEDFEKACITFWAI

XP_030502765.1 uncharacterized protein LOC115717936 [Cannabis sativa]

7.1e-14

32.77

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Query:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS
        +++S S +  WW++ WK KIPPK ++F+WK +H  LP    L++R +  SP C  C S +E+I H L  C R+K + +     +D N     ++ A+ + 
Subjt:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS

Query:  WLSQNLGDEDFEKACITFW
         LS  L   ++E+  +  W
Subjt:  WLSQNLGDEDFEKACITFW

XP_030508852.1 uncharacterized protein LOC115723496 [Cannabis sativa]

1.2e-13

31.4

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Query:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS
        +S+  + +  WW++ WK K+PPK ++F+WK +H  LP    L++R +  SP C  C S +ET+ H L  C R+K +       +D       S  A+ + 
Subjt:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS

Query:  WLSQNLGDEDFEKACITFWAI
         LS +L   + E   +  W+I
Subjt:  WLSQNLGDEDFEKACITFWAI

XP_030509188.1 uncharacterized protein LOC115723863 [Cannabis sativa]

4.6e-13

31.09

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Query:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS
        +++S   +  WW++ WK K+PPK ++F+WK +H  LP    L++R +  SP C  C S +E+I+H L  C R+K +       +D +     ++ A+ + 
Subjt:  ESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERIS

Query:  WLSQNLGDEDFEKACITFW
         LS  L   +FE   +  W
Subjt:  WLSQNLGDEDFEKACITFW

TrEMBL top hits

e value

%identity

Alignment

A0A2N9GM55 Uncharacterized protein

3.0e-18

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Query:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW
        SS S++ +W W  +WK  IP K K FIW+AYHD LPT+H L+KR +  +PLC  C  ++E+  H L  C  ++     +  R+   + + G +FA  + W
Subjt:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW

Query:  LSQNLGDEDFEKACITFWAI
        + QN   ED E+  IT WAI
Subjt:  LSQNLGDEDFEKACITFWAI

A0A2N9HDH5 Uncharacterized protein

1.3e-16

37.5

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Query:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW
        SS S++ +W W  +WK +IP K K FIW+AYHD LPT+  L++R +  +PLC  C  ++E+  H +  C  ++     +  RV   +P+ G +FA  + W
Subjt:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW

Query:  LSQNLGDEDFEKACITFWAI
        + QN   ++ E   IT WAI
Subjt:  LSQNLGDEDFEKACITFWAI

A0A2N9HJA4 zf-RVT domain-containing protein

4.3e-17

37.5

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Query:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW
        SS S++ +W W  +WK +IP K K FIW+AYHD LPT+  L++R +  +PLC  C  ++E+  H L  C  ++     +  RV   +P+ G +FA  + W
Subjt:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW

Query:  LSQNLGDEDFEKACITFWAI
        + QN   ++ E   +T WAI
Subjt:  LSQNLGDEDFEKACITFWAI

A0A2N9HRC9 Uncharacterized protein

1.3e-16

37.5

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Query:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW
        SS S++ +W W  +WK  IP K K FIW+ YHD LPT+H L++R +  +P C  C  ++E+  H L  C  +      +   V   +P+ G +FA  + W
Subjt:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW

Query:  LSQNLGDEDFEKACITFWAI
        + QN   E+ E   IT WAI
Subjt:  LSQNLGDEDFEKACITFWAI

A0A2N9HWG1 Reverse transcriptase domain-containing protein

4.3e-17

37.5

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Query:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW
        SS S++ +W W  +WK +IP K K FIW+AYHD LPT+  L++R +  +PLC  C  ++E+  H L  C  ++     +  RV   +P+ G +FA  + W
Subjt:  SSGSELMKW-WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISW

Query:  LSQNLGDEDFEKACITFWAI
        + QN   ++ E   +T WAI
Subjt:  LSQNLGDEDFEKACITFWAI

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

1.4e-04

27.72

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Query:  LMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISWLSQNLG
        +  ++  LWK ++P + K F+W   +  + T     +R L  S +C  C+   E++ HVL  C     I    + RV       G        WL  NLG
Subjt:  LMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISWLSQNLG

Query:  D
        D
Subjt:  D

Arabidopsis top hits

e value

%identity

Alignment

AT2G02520.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

4.9e-05

27.62

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Query:  ELMKWWTSLW-KSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISWLSQN
        E + W+ ++W K KI PK     W      L T   +   G    PLC+FC +  ET  H+   C+ ++++      RV    P     F + I WL   
Subjt:  ELMKWWTSLW-KSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISWLSQN

Query:  LGDED
          D++
Subjt:  LGDED

AT3G25270.1 Ribonuclease H-like superfamily protein

6.4e-05

32.31

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Query:  LWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNA
        +WK K  PK K F+WK     L T   L +R +   P C  C  + ET  H+   C  ++++  A
Subjt:  LWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNA

AT4G29090.1 Ribonuclease H-like superfamily protein

2.6e-06

37.29

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Query:  WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGC
        +  +WKS+  PK + F+WK   + LP    L  R L     CI C S +ET++H+L  C
Subjt:  WTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGC

Sequences

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CDS sequence

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ATGGAATCAAGTAGTGGCAGCGAATTGATGAAATGGTGGACTTCTTTGTGGAAATCAAAGATCCCACCAAAATGCAAGATGTTCATTTGGAAAGCATACCATGACTGTTT
ACCAACCAATCACTGCTTATGGAAACGAGGCCTCGATATTTCACCGCTATGTATTTTTTGCAGGTCCAAACAGGAAACAATCGACCACGTTCTATGTGGATGCAAGAGAT
CTAAGAAGATATGTAATGCAATGTTCCAAAGGGTGGATGCAAATATCCCTTCTTCGGGAAGTAATTTCGCTGAACGAATATCATGGTTGTCTCAAAATCTTGGAGATGAG
GATTTTGAGAAGGCATGCATCACGTTTTGGGCTATATGTCGCTAG

mRNA sequence

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Protein sequence

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MESSSGSELMKWWTSLWKSKIPPKCKMFIWKAYHDCLPTNHCLWKRGLDISPLCIFCRSKQETIDHVLCGCKRSKKICNAMFQRVDANIPSSGSNFAERISWLSQNLGDE
DFEKACITFWAICR

Lag0031583 (gene) of Sponge gourd (AG-4) v1 genome