CuGenDBv2

Gene ID	Lag0032858
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	chr11:38256434..38256742
RNA-Seq Expression	Lag0032858
Synteny	Lag0032858
Gene Ontology terms	NA
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

XP_010513742.1 PREDICTED: uncharacterized protein LOC104789794 [Camelina sativa]

1.5e-30

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MANFWW + E+ +K+HW+ WE M K KD+GG+GFRDI  FNQALLAK  WRLL  P SLLA++ KARY+P   F++ NLG RPSY WR I++GREL  KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

XP_013654011.1 uncharacterized protein LOC106358754 [Brassica napus]

5.3e-31

62.75

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MA+FWW SSE   KIHWL W+ +  PKDRGGMGF+DI  FNQALLAK  WR+L  P SLL++ LK+RY+P G FL   LGSRPS+ WR I++GREL  KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

XP_013654036.2 uncharacterized protein LOC106358780 [Brassica napus]

4.0e-31

61.76

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MA+FWW SSE   KIHWL W+ +  PKDRGGMGF+DI  FNQALLAK  WR+L  P SL+++ LK+RY+P G FL  +LGSRPS+ WR I++GREL  KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

XP_013709208.1 uncharacterized protein LOC106412876 [Brassica napus]

2.4e-31

62.75

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MA+FWW SSE   KIHWL W+ +  PKDRGGMGF+DI  FNQALLAK  WR+L  P SLL++ LK+RY+P G FL  +LGSRPS+ WR I++GREL  KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

XP_018479968.1 PREDICTED: uncharacterized protein LOC108851032 [Raphanus sativus]

5.3e-31

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MAN+WWG+ E  RKIHWL W+ +  PK+ GGMGFRD+ +FNQALLAK  W++L  P  LLAQ+LK+RY+P  +FL+  LG RPSY WR +++GREL +KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

TrEMBL top hits

e value

%identity

Alignment

A0A1J3FMQ9 Putative mitochondrial protein (Fragment)

8.2e-30

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MA++WW S E  RKIHW  WE +  PK  GGMGFRDI LFNQALLAK  WR+L+ P+ LLA++LK+RY+P+G+FLK  LG+RPS+ WR + +G+EL  +G
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

A0A6D2KLU0 Reverse transcriptase domain-containing protein

2.8e-30

60.78

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MA+FWW S E  RK HW+ WE M  PK  GGMGFRDI  FNQALLAK  W++L  P+SLL + LK+RY+    FL++ +GSRPSY WR IIWGREL +KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

A0A6D2KWQ7 Reverse transcriptase domain-containing protein

1.6e-30

60.78

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MANFWW + E  RKIHW+ WE +  PK  GGMGFRDI  FNQALLAK  W+ L  P SL+A++LK+RY+P+  FL   +GSRPSY WR I+WGREL  KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

A0A6J0LN06 uncharacterized protein LOC108832269

2.2e-30

59.8

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MANFWWGS    RKIHW+ WE +  PKD GGMGFR++  FNQALLAK  W++L  P  LLAQ+LK+RY+P  DFL+  LG RPSY WR ++ GR+L +KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

A0A6J0N7Y1 uncharacterized protein LOC108851032

2.5e-31

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        MAN+WWG+ E  RKIHWL W+ +  PK+ GGMGFRD+ +FNQALLAK  W++L  P  LLAQ+LK+RY+P  +FL+  LG RPSY WR +++GREL +KG
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

2.2e-08

39.39

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Query:  FWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYY
        F WGS+ + +K H +KW  +  PK  GG+G R  +  N+AL++K  WRLL    SL   +L+ +Y+
Subjt:  FWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYY

P93295 Uncharacterized mitochondrial protein AtMg00310

1.8e-26

49.5

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPK-DRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMK
        M  FWW S E  RKI W+ W+ + K K D GG+GFRD+  FNQALLAK ++R++++P +LL+++L++RY+P    ++ ++G+RPSY WR II GREL  +
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPK-DRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMK

Query:  G
        G
Subjt:  G

Arabidopsis top hits

e value

%identity

Alignment

AT4G29090.1 Ribonuclease H-like superfamily protein

1.3e-24

44.12

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG
        +A+FWW + ++ + +HW  W+ ++  K  GG+GF+DI  FN ALL K  WR+L RP+SL+A++ K+RY+ + D L   LGSRPS+VW+ I   +E+  +G
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKG

Query:  GR
         R
Subjt:  GR

ATMG00310.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

1.3e-27

49.5

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Query:  MANFWWGSSEQGRKIHWLKWESMTKPK-DRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMK
        M  FWW S E  RKI W+ W+ + K K D GG+GFRD+  FNQALLAK ++R++++P +LL+++L++RY+P    ++ ++G+RPSY WR II GREL  +
Subjt:  MANFWWGSSEQGRKIHWLKWESMTKPK-DRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMK

Query:  G
        G
Subjt:  G

Sequences

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CDS sequence

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ATGGCTAATTTTTGGTGGGGCAGCTCAGAACAAGGGAGGAAAATTCACTGGTTGAAATGGGAGTCAATGACAAAGCCGAAGGATAGAGGAGGGATGGGGTTCAGAGACAT
AAGACTGTTTAACCAGGCTTTGTTGGCAAAACATACATGGAGGCTTTTATACAGACCTCAATCTCTGTTGGCTCAAATCCTAAAAGCCAGATATTACCCACAAGGAGATT
TCCTTAAAACAAACCTGGGAAGCAGACCATCATATGTCTGGAGGGGGATCATATGGGGAAGAGAACTAGCCATGAAGGGAGGACGCTGA

mRNA sequence

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Protein sequence

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MANFWWGSSEQGRKIHWLKWESMTKPKDRGGMGFRDIRLFNQALLAKHTWRLLYRPQSLLAQILKARYYPQGDFLKTNLGSRPSYVWRGIIWGRELAMKGGR

Lag0032858 (gene) of Sponge gourd (AG-4) v1 genome