CuGenDBv2

Gene ID	Lag0034045
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	protein MIS12 homolog
Genome location	chr3:3955924..3958456
RNA-Seq Expression	Lag0034045
Synteny	Lag0034045
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6589575.1 Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]

3.4e-113

90.79

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDLILGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESP  TS+S DCL DVDLDTELD LRNKLSEVRKEN VLNQELQALERQTASSNSQVN FNEALQLYEQNSVNEMFQEMMGTASELR KIGKLKKRRME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK
        ESKLT+VEK+ TNGD+SHHHKG SNAK DDIQEFLADLK
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK

KAG7023263.1 Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma]

3.8e-112

89.96

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDLILGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESP  TS+S DCL DVDLDTELD LRNKLSEVRKEN VLNQELQALERQ ASSNSQVN FNEALQLYE+NSVNEMFQEMMGTASELR KIGKLKKRRME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK
        ESKLT+VEK+ TNGD+SHHHKG SNAK DDIQEFLADLK
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK

XP_022921372.1 protein MIS12 homolog [Cucurbita moschata]

6.4e-112

89.96

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDLILGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESP  TS+S DCL DVDLDTELD LRNKLSEVRKEN VLNQELQALERQ ASSNSQVN FNEALQLYE+NSVNEMFQEMMGTASELR KIGKLKKRRME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK
        ESKLT+VEK+ TNGD SHHHKG SNAK DDIQEFLADLK
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK

XP_023515498.1 protein MIS12 homolog isoform X1 [Cucurbita pepo subsp. pepo]

1.1e-111

90.04

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDL LGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQ-EMMGTASELRTKIGKLKKRRM
        DESP  TS+S DCL DVDLDTELD LRNKLSEVRKEN VLNQELQALERQTASSNSQVN FNEALQLYEQNSVNEMFQ EMMGTASELR KIGKLKKRRM
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQ-EMMGTASELRTKIGKLKKRRM

Query:  EESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT
        EESKLT+VEK+ TNGD+SHHHKG SNAK DDIQEFLADLKT
Subjt:  EESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

4.5e-113

90.42

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDL LGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESP  TS+S DCL DVDLDTELD LRNKLSEVRKEN VLNQELQALERQTASSNSQVN FNEALQLYEQNSVNEMFQEMMGTASELR KIGKLKKRRME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT
        ESKLT+VEK+ TNGD+SHHHKG SNAK DDIQEFLADLKT
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT

TrEMBL top hits

e value

%identity

Alignment

A0A6J1C2Q6 protein MIS12 homolog

3.2e-109

86.25

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGEAVFDSLNLNPQLFINEALN+VDDLVDDAFDFYQ QAS  LKTEG D+SQDLI+GISRVR+FV S LD+RLAMWEKYCL HCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESPADTSISHDCL DV LDTELDSLRN+LSEVR+EN VLNQELQALERQTASSNSQ+N+FNEAL+LYE+ SVNE FQE+M TASELR KIGKLKKRR+E
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT
        ESKLT+VEKV TNGD+SHHHKG SNAK DDIQEFLADLKT
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT

A0A6J1E0A1 protein MIS12 homolog

3.1e-112

89.96

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDLILGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESP  TS+S DCL DVDLDTELD LRNKLSEVRKEN VLNQELQALERQ ASSNSQVN FNEALQLYE+NSVNEMFQEMMGTASELR KIGKLKKRRME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK
        ESKLT+VEK+ TNGD SHHHKG SNAK DDIQEFLADLK
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK

A0A6J1EZI4 protein MIS12 homolog isoform X1

4.8e-105

84.45

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQSQASTALK+EGSDRSQDLI+GISRVRT  QS LD+RLAMWEKYCL HCFSVPE FSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESPADTSIS+ CL DVDLDTELDSLRNKLSEVRKEN  LNQE QALER+TASSNSQ NYFNEALQLYEQ+SVNEMFQEMMGTAS+LR +IGKLK+R+ME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADL
        +SKLT V+KV TNGD+SHHHKG SNAK DDIQEFLA L
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADL

A0A6J1JD43 protein MIS12 homolog isoform X1

1.3e-110

89.21

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDLILGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQ-EMMGTASELRTKIGKLKKRRM
        DESP  TS+S DCL DVDLDTELD LR  LSEVRKEN VLNQELQALERQTASSNSQVN FNEALQLYEQNSVNEMFQ EMMGTASELR KIGKLKKRRM
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQ-EMMGTASELRTKIGKLKKRRM

Query:  EESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT
        EESKLT+VEK+ TN D+SHHHKG SNAK DDIQEFLADLKT
Subjt:  EESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT

A0A6J1JME3 protein MIS12 homolog isoform X2

5.3e-112

89.58

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QAS +LKTEGSDRSQDLILGISRVRTFVQS LD+RLAMWEKYCL HCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSN

Query:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME
        DESP  TS+S DCL DVDLDTELD LR  LSEVRKEN VLNQELQALERQTASSNSQVN FNEALQLYEQNSVNEMFQEMMGTASELR KIGKLKKRRME
Subjt:  DESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRME

Query:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT
        ESKLT+VEK+ TN D+SHHHKG SNAK DDIQEFLADLKT
Subjt:  ESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLKT

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

5.4e-53

48.96

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSD--RSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLP
        MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD  +SQ L  GI+RVR  + S +D RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSD--RSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLP

Query:  SNDESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRR
         ++ES   +S+  D L D++LD ELDSLR+KL+ V K +  L+ ELQALER + S    +   NEAL+LY+++S++E+F+EM   ASELR  + +LK RR
Subjt:  SNDESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRR

Query:  MEESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK
        M+ S+  +V+++  +G          + K +D+++F A+L+
Subjt:  MEESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

3.8e-54

48.96

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSD--RSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLP
        MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD  +SQ L  GI+RVR  + S +D RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSD--RSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLP

Query:  SNDESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRR
         ++ES   +S+  D L D++LD ELDSLR+KL+ V K +  L+ ELQALER + S    +   NEAL+LY+++S++E+F+EM   ASELR  + +LK RR
Subjt:  SNDESPADTSISHDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRR

Query:  MEESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK
        M+ S+  +V+++  +G          + K +D+++F A+L+
Subjt:  MEESKLTEVEKVDTNGDVSHHHKGLSNAKSDDIQEFLADLK

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAGGGCGAGGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTTATCAATGAAGCTCTCAATATTGTTGACGATTTGGTTGATGACGCTTTCGA
TTTCTATCAATCACAAGCGTCGACCGCCTTGAAGACCGAGGGCTCGGATAGATCTCAGGATCTTATCCTGGGAATATCGCGGGTCCGAACGTTCGTCCAATCGAGTCTGG
ATGAGCGGTTGGCCATGTGGGAGAAGTATTGTCTTTGTCACTGTTTTTCAGTCCCTGAAGGCTTTTCTTTACCCTCAAATGATGAATCACCTGCTGACACCTCAATAAGT
CATGACTGCCTCAATGATGTGGATCTGGATACGGAGTTAGATTCTTTGAGGAATAAGCTCTCTGAGGTCAGAAAAGAGAATTTTGTATTGAACCAAGAACTACAAGCTTT
AGAAAGGCAAACTGCTTCCAGTAACTCCCAAGTCAATTATTTCAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTG
CATCAGAGCTGCGAACGAAAATAGGAAAACTGAAGAAAAGGAGAATGGAGGAATCCAAGCTCACTGAAGTAGAGAAGGTTGATACAAACGGAGACGTCTCTCACCATCAC
AAGGGTTTATCTAATGCTAAGTCGGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCTTTTGA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASTALKTEGSDRSQDLILGISRVRTFVQSSLDERLAMWEKYCLCHCFSVPEGFSLPSNDESPADTSIS
HDCLNDVDLDTELDSLRNKLSEVRKENFVLNQELQALERQTASSNSQVNYFNEALQLYEQNSVNEMFQEMMGTASELRTKIGKLKKRRMEESKLTEVEKVDTNGDVSHHH
KGLSNAKSDDIQEFLADLKTF

Lag0034045 (gene) of Sponge gourd (AG-4) v1 genome