CuGenDBv2

Gene ID	Lag0035709
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	Calcium-binding EF hand family protein
Genome location	chr3:28066635..28067012
RNA-Seq Expression	Lag0035709
Synteny	Lag0035709
Gene Ontology terms	GO:0005509 - calcium ion binding (molecular function)
InterPro domains	IPR002048 - EF-hand domain IPR011992 - EF-hand domain pair IPR018247 - EF-Hand 1, calcium-binding site

Homology

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GenBank top hits

e value

%identity

Alignment

TYJ95513.1 calcium-binding EF hand family protein [Cucumis melo var. makuwa]

7.6e-40

70.08

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     DP+ D+ EVVCHKF V+K+GGID   F S++RDILLAMA G
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK K+
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

XP_004142569.1 uncharacterized protein LOC101222885 [Cucumis sativus]

2.4e-41

70.87

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL V F + PL     DP+ D+ EVVCHKF+V+K+GGID + F S+IRDILLAMAQG
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK ++
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

XP_008462771.1 PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo]

1.5e-40

71.65

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     DP+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA G
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK K+
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

XP_018805702.1 uncharacterized protein LOC108979477 [Juglans regia]

5.5e-22

44.88

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEF---------CNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLA
        MSVA +N +T+  F+ D K F+ CI+E F+ LD+DG+G L+  EL  EF          ++P +   D++E+V  +F+ DKNG ID K FM++IR+++ A
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEF---------CNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLA

Query:  MAQGIGNLPIQVALQEDSFLTKAVEHE
        MA+GIG+ P+ VAL++DS L KA +HE
Subjt:  MAQGIGNLPIQVALQEDSFLTKAVEHE

XP_022158640.1 uncharacterized protein LOC111025096 [Momordica charantia]

6.9e-33

60.66

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLP
        MSVAFLNDTT+ +FINDTK+FD+C+KESF+KLD+D DG LNA+EL   F     DP+ D+ + VC KF V+K+GGI+   F S++ +ILLA+A GIGNLP
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLP

Query:  IQVALQEDSFLTKAVEHEKKKK
        +QVALQ+D  L KAVEHE+ K+
Subjt:  IQVALQEDSFLTKAVEHEKKKK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LYD8 Uncharacterized protein

1.1e-41

70.87

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL V F + PL     DP+ D+ EVVCHKF+V+K+GGID + F S+IRDILLAMAQG
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK ++
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

A0A1S3CI80 uncharacterized protein LOC103501058

7.4e-41

71.65

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     DP+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA G
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK K+
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

A0A5A7VAA7 Calcium-binding EF hand family protein

7.4e-41

71.65

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     DP+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA G
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK K+
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

A0A5D3B8A7 Calcium-binding EF hand family protein

3.7e-40

70.08

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     DP+ D+ EVVCHKF V+K+GGID   F S++RDILLAMA G
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----DPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAVEHEKKKK
        IGN P+QVALQ+DSFL KAVE EK K+
Subjt:  IGNLPIQVALQEDSFLTKAVEHEKKKK

A0A6J1DWE2 uncharacterized protein LOC111025096

3.3e-33

60.66

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLP
        MSVAFLNDTT+ +FINDTK+FD+C+KESF+KLD+D DG LNA+EL   F     DP+ D+ + VC KF V+K+GGI+   F S++ +ILLA+A GIGNLP
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLP

Query:  IQVALQEDSFLTKAVEHEKKKK
        +QVALQ+D  L KAVEHE+ K+
Subjt:  IQVALQEDSFLTKAVEHEKKKK

SwissProt top hits

e value

%identity

Alignment

Q06BI3 Calcium-binding protein 8

9.1e-04

31.75

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Query:  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSII
        D I+E+F+ LD DG+GF++  ELG+   +    P +    ++  + ++D +G +D   FM+I+
Subjt:  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSII

Q9BXU9 Calcium-binding protein 8

9.1e-04

31.75

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Query:  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSII
        D I+E+F+ LD DG+GF++  ELG+   +    P +    ++  + ++D +G +D   FM+I+
Subjt:  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSII

Q9JJG7 Calcium-binding protein 8

9.1e-04

31.75

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Query:  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSII
        D I+E+F+ LD DG+GF++  ELG+   +    P +    ++  + ++D +G +D   FM+I+
Subjt:  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSII

Arabidopsis top hits

e value

%identity

Alignment

AT1G54530.1 Calcium-binding EF hand family protein

4.2e-12

33.87

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Query:  LNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADEL--------GVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIG
        +   TL  F++DTK F+    + F+ LD+D +G L+  EL         VE    P D   ++Y  +  +F  D    +  K F  +I +IL AMA+GIG
Subjt:  LNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADEL--------GVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIG

Query:  NLPIQVALQEDSFLTKAVEHEKKK
        N P+ + +  D  + KAV HE K+
Subjt:  NLPIQVALQEDSFLTKAVEHEKKK

AT2G44310.1 Calcium-binding EF-hand family protein

1.5e-14

30.88

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Query:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQ-------------PLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRD
        M V  ++ +T+ +F++D + F   + E F  LD++ DG L+  EL   F +              P D + ++Y+ +  KF+ D++G +D++ F S ++ 
Subjt:  MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQ-------------PLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRD

Query:  ILLAMAQGIGNLPIQVAL--QEDSFLTKAVEHEKKK
        I+LA+A G+G+ PI + L   +D+FL KA + E  K
Subjt:  ILLAMAQGIGNLPIQVAL--QEDSFLTKAVEHEKKK

AT4G38810.2 Calcium-binding EF-hand family protein

2.2e-05

26.67

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Query:  LNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCN---------QPLDPMKD-IYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG
        L+ + +   + + K+FD  +++ F++LD D DG L+  EL     +         Q   P  D IY  V ++F       +    F  ++ DILL MA G
Subjt:  LNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCN---------QPLDPMKD-IYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQG

Query:  IGNLPIQVALQEDSFLTKAV
        +   PI +   +   L++ V
Subjt:  IGNLPIQVALQEDSFLTKAV

Sequences

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CDS sequence

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ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATCAACGATACAAAGATGTTTGACGATTGCATCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGA
TGGCTTCCTGAATGCCGATGAACTCGGTGTCGAGTTTTGCAATCAACCATTAGATCCGATGAAAGATATCTATGAAGTTGTTTGTCATAAGTTTGAAGTGGACAAAAATG
GTGGAATTGATGTAAAGGGGTTTATGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAAGAAGATAGTTTT
CTCACCAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGCAGAAAAATAG

mRNA sequence

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Protein sequence

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MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSF
LTKAVEHEKKKKAEK

Lag0035709 (gene) of Sponge gourd (AG-4) v1 genome