CuGenDBv2

Gene ID	Lag0040589
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	DNA-directed RNA polymerase
Genome location	chr13:6331122..6336922
RNA-Seq Expression	Lag0040589
Synteny	Lag0040589
Gene Ontology terms	GO:0006351 - transcription, DNA-templated (biological process) GO:0005666 - RNA polymerase III complex (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0032549 - ribonucleoside binding (molecular function) GO:0046872 - metal ion binding (molecular function)
InterPro domains	IPR015712 - DNA-directed RNA polymerase, subunit 2 IPR037033 - DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0051031.1 DNA-directed RNA polymerase III subunit 2 isoform X1 [Cucumis melo var. makuwa]

1.5e-22

77.78

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQARLSC
        V E   + IALYEG+ATPETTHIE+EP TILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQA+  C
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQARLSC

TYK28638.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]

1.1e-22

58.95

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Query:  MRNRTTGKSPFELVYTHLPRLTMDLTNLPSSVTLSPEAEEMADRIQELHHQVHKHIEEQTAKYKTAADNKRRFQEFSIGDLVMVHLRKSRFPTEH
        M NR+TGKSPFE+VYT LP++T DL NLPS + +S EAE MA+RI +LH +V  H+E     YKT A++ +RF+E+ +GDLVMV+L+KSRFPT H
Subjt:  MRNRTTGKSPFELVYTHLPRLTMDLTNLPSSVTLSPEAEEMADRIQELHHQVHKHIEEQTAKYKTAADNKRRFQEFSIGDLVMVHLRKSRFPTEH

XP_004141655.2 DNA-directed RNA polymerase III subunit 2 [Cucumis sativus]

2.5e-22

81.16

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR
        V E   + IALYEG+ATPETTHIE+EPFTILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQ R
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR

XP_038903912.1 DNA-directed RNA polymerase III subunit 2 isoform X1 [Benincasa hispida]

2.5e-22

81.16

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR
        V E   + IALYEG+ATPETTHIE+EPFTILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQ R
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR

XP_038903915.1 DNA-directed RNA polymerase III subunit 2 isoform X3 [Benincasa hispida]

2.5e-22

81.16

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR
        V E   + IALYEG+ATPETTHIE+EPFTILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQ R
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR

TrEMBL top hits

e value

%identity

Alignment

A0A1S3VFK5 DNA-directed RNA polymerase subunit beta

1.6e-22

79.71

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR
        V E   + IALYEGDATPETTHIE+EPFTILGV+A LI YPHHNQSPRNTYQCAM KQA+GNIA+NQ R
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR

A0A3Q0F3D9 DNA-directed RNA polymerase subunit beta

1.6e-22

79.71

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR
        V E   + IALYEGDATPETTHIE+EPFTILGV+A LI YPHHNQSPRNTYQCAM KQA+GNIA+NQ R
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR

A0A3Q0F4M1 DNA-directed RNA polymerase subunit beta

1.6e-22

79.71

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR
        V E   + IALYEGDATPETTHIE+EPFTILGV+A LI YPHHNQSPRNTYQCAM KQA+GNIA+NQ R
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQAR

A0A5A7UBS2 DNA-directed RNA polymerase subunit beta

7.1e-23

77.78

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQARLSC
        V E   + IALYEG+ATPETTHIE+EP TILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQA+  C
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQARLSC

A0A5D3DYV8 DNA/RNA polymerases superfamily protein

5.5e-23

58.95

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Query:  MRNRTTGKSPFELVYTHLPRLTMDLTNLPSSVTLSPEAEEMADRIQELHHQVHKHIEEQTAKYKTAADNKRRFQEFSIGDLVMVHLRKSRFPTEH
        M NR+TGKSPFE+VYT LP++T DL NLPS + +S EAE MA+RI +LH +V  H+E     YKT A++ +RF+E+ +GDLVMV+L+KSRFPT H
Subjt:  MRNRTTGKSPFELVYTHLPRLTMDLTNLPSSVTLSPEAEEMADRIQELHHQVHKHIEEQTAKYKTAADNKRRFQEFSIGDLVMVHLRKSRFPTEH

SwissProt top hits

e value

%identity

Alignment

F4KD38 DNA-directed RNA polymerase III subunit 2

1.4e-20

67.95

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Query:  VLERTMSKIALYEGDATPE-----------TTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ
        V E   + IALYE D T E           TTHIE+EPFTILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQ
Subjt:  VLERTMSKIALYEGDATPE-----------TTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ

P22276 DNA-directed RNA polymerase III subunit RPC2

8.4e-21

74.63

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ
        V E   S IALYE D  P  TH+E+EPFTILG VA LI YPHHNQSPRNTYQCAM KQAIG IAYNQ
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ

P59470 DNA-directed RNA polymerase III subunit RPC2

1.0e-18

67.16

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ
        V E     IALYE     +TTH+E+EPFT+LGV A LI YPHHNQSPRNTYQCAM KQA+G I YNQ
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ

Q10233 DNA-directed RNA polymerase III subunit RPC2

1.3e-21

74.63

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ
        V E   S I++YE D TP+TTH+E+EPFTILG VA LI YPHHNQSPRNTYQCAM KQAIG IAYNQ
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ

Q9NW08 DNA-directed RNA polymerase III subunit RPC2

1.0e-18

67.16

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Query:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ
        V E     IALYE     +TTH+E+EPFT+LGV A LI YPHHNQSPRNTYQCAM KQA+G I YNQ
Subjt:  VLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ

Arabidopsis top hits

e value

%identity

Alignment

AT1G29940.1 nuclear RNA polymerase A2

7.8e-06

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Query:  THIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAI
        TH E+ P  ++ VVA L  +  HNQSPRN YQC MAKQ +
Subjt:  THIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAI

AT4G21710.1 DNA-directed RNA polymerase family protein

1.5e-09

33.83

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Query:  PSITVDDEDRKHNRANFDAIVAKAFASSPVLYRVNLTHFIEEI---QAKARVLERTMSKIALY----EGDATPETTHIEMEPFTILGVVARLILYPHHNQ
        P   VD++     + +  A+  +  A     + +    FIE I   + +  ++  T+S +       E   T   TH E+ P  ILGV A +I +P HNQ
Subjt:  PSITVDDEDRKHNRANFDAIVAKAFASSPVLYRVNLTHFIEEI---QAKARVLERTMSKIALY----EGDATPETTHIEMEPFTILGVVARLILYPHHNQ

Query:  SPRNTYQCAMAKQAIGNIAYN-QARLSCGAKIL
        SPRNTYQ AM KQA+G    N Q R+   A +L
Subjt:  SPRNTYQCAMAKQAIGNIAYN-QARLSCGAKIL

AT5G45140.1 nuclear RNA polymerase C2

1.0e-21

67.95

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Query:  VLERTMSKIALYEGDATPE-----------TTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ
        V E   + IALYE D T E           TTHIE+EPFTILGVVA LI YPHHNQSPRNTYQCAM KQA+GNIAYNQ
Subjt:  VLERTMSKIALYEGDATPE-----------TTHIEMEPFTILGVVARLILYPHHNQSPRNTYQCAMAKQAIGNIAYNQ

Sequences

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CDS sequence

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ATGCGAAACCGAACCACAGGGAAGTCCCCATTTGAGCTTGTGTACACTCATCTTCCTCGCTTAACAATGGACTTAACAAACCTTCCTTCTTCTGTTACTCTTAGTCCTGA
AGCAGAGGAAATGGCGGACAGGATTCAGGAGCTACATCATCAAGTCCACAAACACATTGAAGAACAAACGGCAAAATATAAAACAGCGGCTGACAACAAAAGGCGATTCC
AAGAATTTTCCATTGGAGATCTCGTGATGGTTCATCTACGGAAAAGTCGTTTCCCAACCGAGCACATACAACAAGCTCAAGGAGAAGAAGATTGGTCCGTCTCTTCCCCA
TTCTCAATATTAATGTCTATCAACACCACTACTTCCAATAGGTTTATCGTTCTTGATCTGGTGAATCCCACCACTGAATTTAGAGATAGCTCCGTTGCATCGTCGATTTC
CTGGATGCCCAATGGGCTTCAAGCACATCACCACCTCCGCCTTGATGATTGGGTACCTCATTGGTTAGGTGCAGCAGTGGCACCACCAACTCTTCAAAATTCTCACAGTA
CTTTGTCACCGTCGTTTCCTGCTTCACCACCAGGAAATGCCCACACAATGTTCCTTCTAAATCGTTCCGCTGGATATTTCTTCACGCTTCTCGGTTCCTGGTTTCCACAC
TTCTTCATTGAGATTGATGGTTTCGTCAATTTTGTCTACTCCTTCAACTTTCGACATGATCCTTCTATCACCGTAGATGATGAAGATCGCAAACACAATAGGGCTAACTT
TGACGCAATCGTAGCCAAGGCTTTTGCAAGCTCACCAGTTCTTTACAGAGTCAATTTAACCCATTTTATTGAAGAAATTCAAGCAAAAGCTAGGGTGCTTGAGAGAACCA
TGTCGAAGATTGCTCTGTATGAAGGAGATGCTACACCTGAAACAACCCATATTGAGATGGAGCCCTTTACTATATTAGGTGTTGTAGCTAGGCTAATTCTGTATCCTCAT
CATAACCAGTCGCCTCGAAACACGTATCAGTGCGCTATGGCTAAGCAAGCCATCGGAAATATTGCATACAATCAGGCAAGACTCTCATGTGGAGCTAAGATATTAGATGA
TGATGGACTTGCTGCACCGTAG

mRNA sequence

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Protein sequence

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MRNRTTGKSPFELVYTHLPRLTMDLTNLPSSVTLSPEAEEMADRIQELHHQVHKHIEEQTAKYKTAADNKRRFQEFSIGDLVMVHLRKSRFPTEHIQQAQGEEDWSVSSP
FSILMSINTTTSNRFIVLDLVNPTTEFRDSSVASSISWMPNGLQAHHHLRLDDWVPHWLGAAVAPPTLQNSHSTLSPSFPASPPGNAHTMFLLNRSAGYFFTLLGSWFPH
FFIEIDGFVNFVYSFNFRHDPSITVDDEDRKHNRANFDAIVAKAFASSPVLYRVNLTHFIEEIQAKARVLERTMSKIALYEGDATPETTHIEMEPFTILGVVARLILYPH
HNQSPRNTYQCAMAKQAIGNIAYNQARLSCGAKILDDDGLAAP

Lag0040589 (gene) of Sponge gourd (AG-4) v1 genome