CuGenDBv2

Gene ID	Lag0042094
Organism	Luffa acutangula AG-4 (Sponge gourd (AG-4) v1)
Description	mitotic spindle checkpoint protein MAD1
Genome location	chr13:36340398..36353804
RNA-Seq Expression	Lag0042094
Synteny	Lag0042094
Gene Ontology terms	GO:0007094 - mitotic spindle assembly checkpoint (biological process) GO:0005634 - nucleus (cellular component)
InterPro domains	IPR008672 - Spindle assembly checkpoint component Mad1

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0054160.1 mitotic spindle checkpoint protein MAD1 [Cucumis melo var. makuwa]

4.5e-60

80.59

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQIDKQKA++EATL KEKIEALKL  +QNELLLS+ATEERDKLKS       LKNDDAEA   + TLSQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS  AKKDWYIKELESNLHEQKEVNSRQHDE++LLNERLNN+AKRIKSLERD DRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

KAG6601670.1 Mitotic spindle checkpoint protein MAD1, partial [Cucurbita argyrosperma subsp. sororia]

2.7e-60

81.18

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQ DKQKA++EATLAKEKIEALKL  +QNELLLS+ATEERDK       LKSLKN DAEA   S T+SQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS LAKKDWYIKELESNLHEQK+VN RQHDE+RLLNERLNNEAKRIKSLERDSDRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

XP_004142360.1 mitotic spindle checkpoint protein MAD1 isoform X1 [Cucumis sativus]

3.5e-60

81.18

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQIDKQKA++EATL +EKIEALKL  KQNELLLS+ATEERDKLKS       LKNDDAEA     TLSQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELD TLAKKDWYIKELESNLHEQKEVNSRQHDE++LLNERLNN+AKRIKSLERD DRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

XP_022997454.1 mitotic spindle checkpoint protein MAD1 [Cucurbita maxima]

2.7e-60

81.18

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQ DKQKA++EATLAKEKIEALKL  +QNELLLS+ATEERDK       LKSLKN DAEA   S T+SQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS LAKKDWYIKELESNLHEQK+VN RQHDE+RLLNERLNNEAKRIKSLERDSDRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

XP_031741821.1 mitotic spindle checkpoint protein MAD1 isoform X2 [Cucumis sativus]

3.5e-60

81.18

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQIDKQKA++EATL +EKIEALKL  KQNELLLS+ATEERDKLKS       LKNDDAEA     TLSQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELD TLAKKDWYIKELESNLHEQKEVNSRQHDE++LLNERLNN+AKRIKSLERD DRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KV95 Uncharacterized protein

1.7e-60

81.18

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQIDKQKA++EATL +EKIEALKL  KQNELLLS+ATEERDKLKS       LKNDDAEA     TLSQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELD TLAKKDWYIKELESNLHEQKEVNSRQHDE++LLNERLNN+AKRIKSLERD DRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

A0A1S3CCD1 mitotic spindle checkpoint protein MAD1

2.2e-60

80.59

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQIDKQKA++EATL KEKIEALKL  +QNELLLS+ATEERDKLKS       LKNDDAEA   + TLSQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS  AKKDWYIKELESNLHEQKEVNSRQHDE++LLNERLNN+AKRIKSLERD DRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

A0A5A7UFY3 Mitotic spindle checkpoint protein MAD1

2.2e-60

80.59

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQIDKQKA++EATL KEKIEALKL  +QNELLLS+ATEERDKLKS       LKNDDAEA   + TLSQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKS-------LKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS  AKKDWYIKELESNLHEQKEVNSRQHDE++LLNERLNN+AKRIKSLERD DRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

A0A6J1EY58 mitotic spindle checkpoint protein MAD1

4.9e-60

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Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQ DKQKA++EATLA+EKIEAL+L  +QNELLLS+ATEERDK       LKSLKN DAEA   S T+SQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS LAKKDWYIKELESNLHEQK+VN RQHDE+RLLNERLNNEAKRIKSLERDSDRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

A0A6J1KBD3 mitotic spindle checkpoint protein MAD1

1.3e-60

81.18

Show/hide

Query:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ
        I  +++EVIDNT+KMGE+NARLKQLEVALDAAQ DKQKA++EATLAKEKIEALKL  +QNELLLS+ATEERDK       LKSLKN DAEA   S T+SQ
Subjt:  IVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDK-------LKSLKNDDAEALVASGTLSQ

Query:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        ELDS LAKKDWYIKELESNLHEQK+VN RQHDE+RLLNERLNNEAKRIKSLERDSDRLRSEISLLESK G
Subjt:  ELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

SwissProt top hits

e value

%identity

Alignment

Q9LTY1 Mitotic spindle checkpoint protein MAD1

3.4e-34

53.29

Show/hide

Query:  VRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKSLKND----DAEALV---ASGTLSQELD
        ++ EV+ +T+K+GE + R+KQLE  L+A Q+ +Q A +EA LAKEK EALK   K+ E++L++ TEE+++LK++ N+    ++E  V   A G L Q  +
Subjt:  VRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKSLKND----DAEALV---ASGTLSQELD

Query:  STLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        S+LAKK+ YIK+LE +L++ K+VN+RQ  EI LLNE+L +EA+R KSLERDSDRLRSEISLLESK G
Subjt:  STLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

Arabidopsis top hits

e value

%identity

Alignment

AT5G49880.1 mitotic checkpoint family protein

2.4e-35

53.29

Show/hide

Query:  VRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKSLKND----DAEALV---ASGTLSQELD
        ++ EV+ +T+K+GE + R+KQLE  L+A Q+ +Q A +EA LAKEK EALK   K+ E++L++ TEE+++LK++ N+    ++E  V   A G L Q  +
Subjt:  VRREVIDNTVKMGELNARLKQLEVALDAAQIDKQKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKSLKND----DAEALV---ASGTLSQELD

Query:  STLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG
        S+LAKK+ YIK+LE +L++ K+VN+RQ  EI LLNE+L +EA+R KSLERDSDRLRSEISLLESK G
Subjt:  STLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKEG

Sequences

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CDS sequence

Show/hide CDS sequence

ATGAGATTAGTTGGGCGCGCACCTTTATCCATGGCAAAGGAGTCGACAAGTGATGCAAGAACCCTAGCCACCATCCATTACATGCTCCCTACTTTTGGCATGACACCACA
CCCATCTTCTTCGTTTGCTCGCAGATTTGCAACCACCTTATTTGCAAATCAATCCACTTGCAAGTTTGCCGTCCATTCGCAATTCGTTGTTTGTCTGCCTAGCTGGTTCA
ACATCGTTCATGTTCGGAGGGAGGTAATTGATAACACAGTAAAGATGGGTGAGTTGAATGCTCGCTTGAAACAATTGGAAGTAGCACTGGATGCAGCTCAAATTGATAAG
CAAAAGGCACAAACTGAGGCTACACTTGCAAAAGAAAAGATTGAAGCATTAAAGTTGGGAGCTAAGCAGAATGAATTGCTTCTCTCCATGGCGACTGAAGAAAGGGATAA
ATTGAAAAGTCTGAAAAATGATGATGCAGAGGCTTTAGTAGCGAGTGGAACTCTTTCTCAGGAGCTAGACTCAACCCTTGCAAAGAAAGATTGGTATATTAAAGAATTAG
AAAGTAATTTACATGAGCAAAAAGAGGTTAATAGCCGACAACATGATGAGATAAGGTTGTTGAATGAGAGATTAAATAATGAAGCAAAAAGAATAAAGTCATTGGAGAGG
GATTCTGATCGGCTTCGTTCTGAAATTTCTCTATTGGAGTCAAAGGAAGGAGCGCCGCCGTCAGGAGAGTGGAAGCGCCGTCGCCGATCGTCGGAAAACGCCGCGCGTGA
GTCACACGCGCCGCCGAAGGGATCTGCAGCCGCCGCCACGCGCCGGCGCGTGAGGGCGTGTGGGCCGTCCGTTTCTGTTTCCGCCGCCTGGTTTTTCAGCGGTTTTAAGC
TACCAATGGCTGAGCTGAAACCTTTGGTCATATCTAATGGAGTTCCCCTTTCTTCGAAGTTCACAGAACATAAGCTAAATGGCTCCAATTACTATGAATAG

mRNA sequence

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Protein sequence

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MRLVGRAPLSMAKESTSDARTLATIHYMLPTFGMTPHPSSSFARRFATTLFANQSTCKFAVHSQFVVCLPSWFNIVHVRREVIDNTVKMGELNARLKQLEVALDAAQIDK
QKAQTEATLAKEKIEALKLGAKQNELLLSMATEERDKLKSLKNDDAEALVASGTLSQELDSTLAKKDWYIKELESNLHEQKEVNSRQHDEIRLLNERLNNEAKRIKSLER
DSDRLRSEISLLESKEGAPPSGEWKRRRRSSENAARESHAPPKGSAAAATRRRVRACGPSVSVSAAWFFSGFKLPMAELKPLVISNGVPLSSKFTEHKLNGSNYYE

Lag0042094 (gene) of Sponge gourd (AG-4) v1 genome