CuGenDBv2

Gene ID	Lcy04g018780
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Genome location	Chr04:50620588..50626303
RNA-Seq Expression	Lcy04g018780
Synteny	Lcy04g018780
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

KAF4358383.1 hypothetical protein G4B88_008543 [Cannabis sativa]

2.1e-15

31.1

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Query:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN
        A K  H        CW+PP  G + +NTDA+  +  P  GLG I RD +GE+  AA+ ++   L  +M E   I   M++A SR  + I + SD+Q  I 
Subjt:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN

Query:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPS
         ++ ++   +D   ++      ++ F+ +SF F PR  N  A  LA +++     + W S LP+
Subjt:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPS

KAF4363590.1 hypothetical protein F8388_002131 [Cannabis sativa]

6.0e-15

31.06

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Query:  KRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFI
        K  H        CW+PP  G + +NTDA+  +  P  GLG+I RD +GE+  AA+ ++   L  +M E   I   M++A SR  + I + SD+Q  I  +
Subjt:  KRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFI

Query:  QRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
        + ++   +D   ++      ++ F+ +SF F PR  N  A  LA +++     + W S LP
Subjt:  QRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

KAF4381263.1 hypothetical protein G4B88_009591 [Cannabis sativa]

6.0e-15

30.67

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Query:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN
        A K  H        CW+PP  G + +NTDA+  +  P  GLG+I RD +GE+  AA+ ++   L  +M E   I   +++A SR  + I + SD+Q  I 
Subjt:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN

Query:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
         ++ ++   +D   ++      A+ F+ +SF F PR  N  A   A +++     + W S LP
Subjt:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

OMO58663.1 reverse transcriptase [Corchorus capsularis]

4.1e-16

37.16

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Query:  WTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIWSDVEAL
        W  PS G WK+NTDA+ S S    GLG++ RD  G++ V+ +  L +  DS+  E+  IL G  +AL  G  +  +ESDS  AI+ I +K  +  +   L
Subjt:  WTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIWSDVEAL

Query:  VTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
        +  I E+A LF   SF+F+ R  N  A SLA     +K    W   +P
Subjt:  VTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

XP_030478193.1 uncharacterized protein LOC115695254 [Cannabis sativa]

1.0e-14

30.67

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Query:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN
        A K  H        CW+PP  G + +NTDA+  +  P  GLG+I RD +GE+  AA+ ++   L  +M E   I   +++A SR  + I + SD+Q  I 
Subjt:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN

Query:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
         ++ ++   +D   ++      A+ F+ +SF F PR  N  A   A +++     + W S LP
Subjt:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

TrEMBL top hits

e value

%identity

Alignment

A0A1R3GKQ5 Reverse transcriptase

2.0e-16

37.16

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Query:  WTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIWSDVEAL
        W  PS G WK+NTDA+ S S    GLG++ RD  G++ V+ +  L +  DS+  E+  IL G  +AL  G  +  +ESDS  AI+ I +K  +  +   L
Subjt:  WTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIWSDVEAL

Query:  VTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
        +  I E+A LF   SF+F+ R  N  A SLA     +K    W   +P
Subjt:  VTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

A0A7J6EJ00 RNase H domain-containing protein

1.0e-15

31.1

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Query:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN
        A K  H        CW+PP  G + +NTDA+  +  P  GLG I RD +GE+  AA+ ++   L  +M E   I   M++A SR  + I + SD+Q  I 
Subjt:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN

Query:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPS
         ++ ++   +D   ++      ++ F+ +SF F PR  N  A  LA +++     + W S LP+
Subjt:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPS

A0A7J6F1G1 Uncharacterized protein

2.9e-15

31.06

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Query:  KRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFI
        K  H        CW+PP  G + +NTDA+  +  P  GLG+I RD +GE+  AA+ ++   L  +M E   I   M++A SR  + I + SD+Q  I  +
Subjt:  KRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFI

Query:  QRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
        + ++   +D   ++      ++ F+ +SF F PR  N  A  LA +++     + W S LP
Subjt:  QRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

A0A7J6GEF1 RNase H domain-containing protein

2.9e-15

30.67

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Query:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN
        A K  H        CW+PP  G + +NTDA+  +  P  GLG+I RD +GE+  AA+ ++   L  +M E   I   +++A SR  + I + SD+Q  I 
Subjt:  AAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAIN

Query:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP
         ++ ++   +D   ++      A+ F+ +SF F PR  N  A   A +++     + W S LP
Subjt:  FIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLP

A0A803PWX1 Uncharacterized protein

7.6e-16

34.32

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Query:  RNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIW
        R  +A WTPP  G +K+N DA         GL  + RD  G V VAA+ F++ +   + GEL+ IL G++  + R      VESD  QA+N + ++    
Subjt:  RNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIW

Query:  SDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPSWLS--IGPDGPFSL
         DV+ L+  I EL Q        F+ R  N  A  LA  A   K    W  V+P   S  I  D P+ L
Subjt:  SDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPSWLS--IGPDGPFSL

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT4G09490.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.6e-05

34.57

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Query:  IMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAK
        +M E   +   ++ A S G  K+ + SDSQQ I  I  +S   ++   ++  I  L+  F+++SF F+PR +N  AD LAK
Subjt:  IMGELRGILEGMRIALSRGCEKIEVESDSQQAINFIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAK

AT4G29090.1 Ribonuclease H-like superfamily protein

1.6e-10

28.8

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Query:  FQVPTNASSSSTRIAAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMR---IALS
        +++ T A S  T+         +R+    W PP   + K NTDA  +      G+G + R++ GEVK   +      L  +   L   LE MR   ++LS
Subjt:  FQVPTNASSSSTRIAAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMR---IALS

Query:  R-GCEKIEVESDSQQAINFIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFA-KFTKCRETWDSVLPSW
        R     +  ESDSQ  I  +     IW  ++  +  +  L   F+E+ F FIPR  N  A+ +A+ +  F        S++PSW
Subjt:  R-GCEKIEVESDSQQAINFIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFA-KFTKCRETWDSVLPSW

Sequences

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CDS sequence

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ATGGGACTGTTTCAAGTTCCCACTAATGCTTCTTCTTCCTCGACTAGAATTGCAGCCAAGCGCCTCCATCCTCATGACCGAAATGGTGATGCATGCTGGACTCCC
CCTTCCGATGGATTCTGGAAGCTAAATACAGATGCAGCGTGTTCCGATTCTCCTCCTTACACGGGGTTAGGCATGATCTGTAGAGACAAAATCGGTGAAGTGAAG
GTGGCAGCATCTATTTTTCTGGATTACCGGTTGGATTCGATAATGGGAGAGTTGAGAGGAATTTTGGAAGGGATGAGGATAGCTCTGAGTAGGGGCTGTGAGAAA
ATCGAAGTGGAGTCTGATAGTCAACAAGCCATTAATTTCATCCAGCGAAAATCTATCATTTGGAGTGACGTGGAAGCTTTAGTGACATCCATCTGGGAGCTAGCG
CAATTATTTTCTGAGATTTCGTTCAAATTCATTCCAAGGATGCAAAATGGAGAAGCAGATTCGTTAGCTAAATTCGCGAAATTCACAAAATGTAGAGAGACTTGG
GATTCTGTACTCCCAAGTTGGCTGAGTATTGGGCCTGATGGGCCTTTTTCTTTGCCCTTGTGGCGTTAA

mRNA sequence

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Protein sequence

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MGLFQVPTNASSSSTRIAAKRLHPHDRNGDACWTPPSDGFWKLNTDAACSDSPPYTGLGMICRDKIGEVKVAASIFLDYRLDSIMGELRGILEGMRIALSRGCEK
IEVESDSQQAINFIQRKSIIWSDVEALVTSIWELAQLFSEISFKFIPRMQNGEADSLAKFAKFTKCRETWDSVLPSWLSIGPDGPFSLPLWR

Lcy04g018780 (gene) of Sponge gourd (P93075) v1 genome