CuGenDBv2

Gene ID	Lcy06g019300
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	mediator of RNA polymerase II transcription subunit 27
Genome location	Chr06:43904439..43908079
RNA-Seq Expression	Lcy06g019300
Synteny	Lcy06g019300
Gene Ontology terms	GO:0006357 - regulation of transcription by RNA polymerase II (biological process) GO:0045893 - positive regulation of transcription, DNA-templated (biological process) GO:0016592 - mediator complex (cellular component) GO:0003713 - transcription coactivator activity (molecular function)
InterPro domains	IPR021627 - Mediator complex, subunit Med27

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6596615.1 Mediator of RNA polymerase II transcription subunit 27, partial [Cucurbita argyrosperma subsp. sororia]

8.6e-72

89.82

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS+GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

XP_022151438.1 mediator of RNA polymerase II transcription subunit 27 [Momordica charantia]

9.2e-74

91.02

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQQPAQGQTATVSPNTA PHS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

XP_022940393.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata]

8.6e-72

89.82

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS+GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

XP_023539656.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pepo]

8.6e-72

89.82

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS+GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

XP_038904949.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida]

3.7e-75

91.62

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQQPAQGQTATVSPNTAPPHS  SA++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

TrEMBL top hits

e value

%identity

Alignment

A0A1S3B7L6 mediator of RNA polymerase II transcription subunit 27

5.1e-70

87.43

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  QQP+QGQTATVSPNT   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

A0A5D3DNW2 Mediator of RNA polymerase II transcription subunit 27

5.1e-70

87.43

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  QQP+QGQTATVSPNT   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

A0A6J1DC62 mediator of RNA polymerase II transcription subunit 27

4.4e-74

91.02

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQQPAQGQTATVSPNTA PHS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

A0A6J1FP59 mediator of RNA polymerase II transcription subunit 27

4.2e-72

89.82

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS+GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

A0A6J1KZZ9 mediator of RNA polymerase II transcription subunit 27

4.2e-72

89.82

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS+GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVF

SwissProt top hits

e value

%identity

Alignment

Q8RWM3 Mediator of RNA polymerase II transcription subunit 27

4.3e-50

69.93

Show/hide

Query:  NTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNS
        N A   +  S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDASMRQHL DLR +GK+LEESGVL+ESL SRSNS
Subjt:  NTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNS

Query:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVFASI
        WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F  I
Subjt:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVFASI

Arabidopsis top hits

e value

%identity

Alignment

AT3G09180.1 CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).

3.1e-51

69.93

Show/hide

Query:  NTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNS
        N A   +  S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDASMRQHL DLR +GK+LEESGVL+ESL SRSNS
Subjt:  NTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNS

Query:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVFASI
        WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR  +K   DQ    F  I
Subjt:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTR-DMKLTNDQVENVFASI

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGCGACAGACGCAGCAGCCTGCGCAGGGCCAGACTGCGACGGTGAGCCCTAACACTGCTCCGCCTCATTCTGCCGGCTCCGCGGAGGAGGCGCCGCCGAAGCAGGTAGC
ACTCGCCATGGACAGACTCGGCCACGCCGCCCGCCTTATCGCCGACATCAGGCTCGGCGCCGATCGCCTTCTCGAAGCCCTCTGCGTCACTGCTCAGCCTCACCAGAGCA
CCAAGCCTCTCCACTTGTTTCAGAAAGAAGACGCCTCCATGCGTCAACACCTTCTTGACCTTCGTGCAGTTGGGAAGCAGCTTGAAGAGTCTGGTGTTCTCAGTGAATCT
CTTCTTTCGAGAAGTAATTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCAGTGGTCGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCTGGTGCATC
CGCAGTTGATCGGACCAGAGATATGAAGTTGACTAATGATCAAGTTGAAAACGTTTTTGCTTCTATTTCACTGTCAGTCGCCATTAAATTTGAATTTGATAGACCAAATC
TTGGCCTTGTGGACTCTGATTCACATGTTATAGGATATCGTGTTCTCTATGAATTAGTATGCTCAGAATCAGAAGGTGCAGCCTGCTTGCTATCGTGTGCTCCTTTTGGT
GATTCACTAGCCTGTGCATTATGTTTCATTTCAGGTGACATCCTGCGAAAAAATCTCATTTGTAAATCATAA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MRQTQQPAQGQTATVSPNTAPPHSAGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSES
LLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRDMKLTNDQVENVFASISLSVAIKFEFDRPNLGLVDSDSHVIGYRVLYELVCSESEGAACLLSCAPFG
DSLACALCFISGDILRKNLICKS

Lcy06g019300 (gene) of Sponge gourd (P93075) v1 genome