CuGenDBv2

Gene ID	Lsi01G016810
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Threonylcarbamoyladenosine tRNA methylthiotransferase
Genome location	chr01:15392851..15396994
RNA-Seq Expression	Lsi01G016810
Synteny	Lsi01G016810
Gene Ontology terms	GO:0035600 - tRNA methylthiolation (biological process) GO:0005783 - endoplasmic reticulum (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0035598 - N6-threonylcarbomyladenosine methylthiotransferase activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function) GO:0061712 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase (molecular function)
InterPro domains	IPR013848 - Methylthiotransferase, N-terminal IPR038135 - Methylthiotransferase, N-terminal domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0039222.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo var. makuwa]

4.5e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

XP_004141564.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucumis sativus]

4.5e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

XP_008459673.1 PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo]

4.5e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

XP_008459674.1 PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Cucumis melo]

5.9e-09

91.43

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Query:  SDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  SDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

XP_038890156.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida]

4.5e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KSZ4 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.2e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

A0A1S3CA89 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.2e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

A0A1S3CAU3 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.9e-09

91.43

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Query:  SDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  SDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

A0A5D3BN67 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.2e-09

88.89

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP+DADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

A0A6J1DPN3 Threonylcarbamoyladenosine tRNA methylthiotransferase

6.4e-09

86.11

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        +SD+EYMAGQLSAFGYLLSDNP++ADLWLINTCT K
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

SwissProt top hits

e value

%identity

Alignment

Q5VV42 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.3e-08

51.79

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Query:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        D  + G Q I   TW      SD EYMAGQL+A+GY +++N  DADLWL+N+CT K
Subjt:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

Q6NS26 Threonylcarbamoyladenosine tRNA methylthiotransferase

4.0e-08

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Query:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        D  + G Q I   TW      SD EYMAGQL+A+GY +++ P+ ADLWL+N+CT K
Subjt:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

Q6P4Y0 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.0e-08

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Query:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        D  + G Q I   TW      SD EYMAGQL+A+GY +++ P+ ADLWL+N+CT K
Subjt:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

Q6PG34 Threonylcarbamoyladenosine tRNA methylthiotransferase

7.5e-07

43.33

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Query:  RIDIDRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        ++  D  + G Q +   TW      SD EYMAGQL+  GY ++++  DADLWL+N+CT K
Subjt:  RIDIDRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

Q91WE6 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.3e-08

51.79

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Query:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK
        D  + G Q I   TW      SD EYMAGQL+A+GY +++N  DADLWL+N+CT K
Subjt:  DRPMGGRQLIENMTW--QRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRK

Arabidopsis top hits

e value

%identity

Alignment

AT1G72090.1 Methylthiotransferase

4.4e-10

43.37

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Query:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRKVALRFPVAGKAWRRLPCDRDIGSSSSSSSSISRGELALKPFIFDSSV
        +SD+EYMAGQLSAFGY L++ P++ADLWLINTCT K                      S S+ S+ I+RG    KP +    V
Subjt:  RSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRKVALRFPVAGKAWRRLPCDRDIGSSSSSSSSISRGELALKPFIFDSSV

Sequences

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CDS sequence

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ATGACATTATTCGAGTGCGTTCACATGCCTAATGAAAGGTTTCGAATTGACATCGACAGACCAATGGGAGGCCGCCAACTCATAGAGAATATGACGTGGCAGAGGCTCCG
CAGTGACAACGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGCGACAATCCTAAAGATGCAGACTTGTGGCTTATAAATACTTGCACACGGAAAGTTG
CACTTCGGTTTCCAGTTGCAGGGAAGGCATGGCGTCGTTTGCCTTGCGACAGAGACATCGGTTCGTCATCGTCGTCGTCATCATCAATCTCAAGAGGTGAACTAGCACTA
AAACCTTTCATCTTTGACTCATCGGTGGCTTCTGCAACAGGAGGCAATTCTTCATCTTCGTCTTCGTCTTCGTCATCTTCATCTGAATCATCTTCCAACATGATAATCTC
GTCTGTCGGGAAGGGTTTTGGAACTGCAATTGCAGGAGGCTCTACATTCAAGCCTTTTGCTGACTCGGCAGCAGGCAATACAAGTGAGGCAGCTAACAGGGGAAGAAGTA
AAAGAAATGGTATCTTACACACTACACCAAAGGGACCCACCTTTTTATCAGTATCTAATTTTCTCTTCGGGGCCGAGTTCCCCAATAGCGACCCAAGACCTCCTCGGTAC
AAAAGGCCGGTATCCACGTTCCAAGGGTGCAACTTTAGACTGAGAAGAGGGTGA

mRNA sequence

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Protein sequence

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MTLFECVHMPNERFRIDIDRPMGGRQLIENMTWQRLRSDNEYMAGQLSAFGYLLSDNPKDADLWLINTCTRKVALRFPVAGKAWRRLPCDRDIGSSSSSSSSISRGELAL
KPFIFDSSVASATGGNSSSSSSSSSSSSESSSNMIISSVGKGFGTAIAGGSTFKPFADSAAGNTSEAANRGRSKRNGILHTTPKGPTFLSVSNFLFGAEFPNSDPRPPRY
KRPVSTFQGCNFRLRRG

Lsi01G016810 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome