CuGenDBv2

Gene ID	Lsi01G020220
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Translation factor GUF1 homolog, chloroplastic
Genome location	chr01:27645742..27650514
RNA-Seq Expression	Lsi01G020220
Synteny	Lsi01G020220
Gene Ontology terms	GO:0006414 - translational elongation (biological process) GO:0045727 - positive regulation of translation (biological process) GO:0005743 - mitochondrial inner membrane (cellular component) GO:0005759 - mitochondrial matrix (cellular component) GO:0009507 - chloroplast (cellular component) GO:0003746 - translation elongation factor activity (molecular function) GO:0003924 - GTPase activity (molecular function) GO:0005525 - GTP binding (molecular function) GO:0043022 - ribosome binding (molecular function)
InterPro domains	IPR006297 - Elongation factor 4 IPR013842 - GTP-binding protein LepA, C-terminal IPR038363 - LepA, C-terminal domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6595718.1 Translation factor GUF1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]

5.1e-56

88.57

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

KAG7027680.1 Translation factor GUF1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma]

5.1e-56

88.57

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

XP_022925039.1 translation factor GUF1 homolog, chloroplastic [Cucurbita moschata]

5.1e-56

88.57

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Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

XP_022966535.1 translation factor GUF1 homolog, chloroplastic [Cucurbita maxima]

5.1e-56

88.57

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Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

XP_023518730.1 translation factor GUF1 homolog, chloroplastic [Cucurbita pepo subsp. pepo]

5.1e-56

88.57

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Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KYH8 Translation factor GUF1 homolog, mitochondrial

7.2e-56

87.14

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPL+TIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSK+IASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

A0A1S3BMN9 Translation factor GUF1 homolog, chloroplastic

5.5e-56

87.86

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Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPL+TIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

A0A5A7V5D5 Translation factor GUF1 homolog, mitochondrial

5.5e-56

87.86

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPL+TIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

A0A6J1EAZ6 Translation factor GUF1 homolog, chloroplastic

2.5e-56

88.57

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

A0A6J1HRW4 Translation factor GUF1 homolog, chloroplastic

2.5e-56

88.57

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRESELIKLDIQINGDRVEPLATIVH+NK               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

SwissProt top hits

e value

%identity

Alignment

A5B4D2 Translation factor GUF1 homolog, chloroplastic

5.9e-55

81.43

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        Y+ESELIKLDIQING+RVEPLATIVH +K               AY+VGRALTQKLKELIPRQMFKVPIQACIG+KVIASE++SAIRKDVL+KCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLL+KQAEGKKRMKAIG+VDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

B8AI54 Translation factor GUF1 homolog, chloroplastic

1.2e-52

79.29

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRES L+KLDIQINGD VE L+TIVH +K               AYSVGRALTQKLKELIPRQMF+VPIQACIG+KVIASE +SAIRKDVL+KCYGGDI+
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLL+KQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

B9F2U5 Translation factor GUF1 homolog, chloroplastic

1.2e-52

79.29

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRES L+KLDIQINGD VE L+TIVH +K               AYSVGRALTQKLKELIPRQMF+VPIQACIG+KVIASE +SAIRKDVL+KCYGGDI+
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLL+KQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

B9GHA6 Translation factor GUF1 homolog, chloroplastic

1.9e-53

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        Y+ES+LI+LDIQINGD VEPLATIVH +K               AYSVGRALTQKLKELIPRQMFKVPIQACIG+KVIASE++SAIRKDVLAKCYGGDI+
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLL+KQA GKKRMKAIG+VDVPQEAFMAVLKLEKEVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

Q9FNM5 Translation factor GUF1 homolog, chloroplastic

4.6e-52

79.29

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRES+LIKLDI IN + VEPL+TIVH +K               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASE +SAIRKDVLAKCYGGDI+
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLL+KQA GKKRMKAIGRVDVPQEAFMAVLKLE+EVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

Arabidopsis top hits

e value

%identity

Alignment

AT5G08650.1 Small GTP-binding protein

3.3e-53

79.29

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        YRES+LIKLDI IN + VEPL+TIVH +K               AYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASE +SAIRKDVLAKCYGGDI+
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL
        RKKKLL+KQA GKKRMKAIGRVDVPQEAFMAVLKLE+EVL
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

AT5G39900.1 Small GTP-binding protein

1.9e-37

Show/hide

Query:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT
        Y+ S+L+KLDI +NG  V+ LATIVH  K               AY VG+ L +KLK  I RQMF+V IQA IGSK+IA +TISA+RK+VLAKCYGGDIT
Subjt:  YRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDIT

Query:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKL
        RKKKLL KQ EGKKRMK +G VD+P EAF  +LK+
Subjt:  RKKKLLRKQAEGKKRMKAIGRVDVPQEAFMAVLKL

Sequences

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CDS sequence

Show/hide CDS sequence

ATGACTTTTAGGTACAGAGAAAGTGAATTGATAAAACTGGACATTCAGATAAATGGTGATCGAGTGGAACCATTGGCAACAATAGTGCACAGCAATAAGACAAACTTGCT
ATGCTATGAAGTTGAAAAGAAGTACTGTTTTCAGGCATATTCTGTCGGAAGAGCTTTGACTCAGAAGCTGAAGGAATTGATACCACGACAAATGTTTAAAGTACCTATTC
AGGCCTGTATTGGCTCTAAAGTGATTGCTAGTGAAACTATATCAGCAATTAGAAAGGATGTCCTTGCCAAATGCTACGGTGGAGACATAACAAGGAAGAAGAAATTGCTT
AGGAAACAGGCTGAAGGAAAGAAGAGAATGAAAGCGATTGGGAGAGTTGATGTACCTCAAGAAGCTTTTATGGCTGTTTTGAAGCTTGAAAAGGAGGTTTTGTAA

mRNA sequence

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Protein sequence

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MTFRYRESELIKLDIQINGDRVEPLATIVHSNKTNLLCYEVEKKYCFQAYSVGRALTQKLKELIPRQMFKVPIQACIGSKVIASETISAIRKDVLAKCYGGDITRKKKLL
RKQAEGKKRMKAIGRVDVPQEAFMAVLKLEKEVL

Lsi01G020220 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome