CuGenDBv2

Gene ID	Lsi02G011620
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	tRNA (Guanine(9)-N1)-methyltransferase
Genome location	chr02:14026398..14031308
RNA-Seq Expression	Lsi02G011620
Synteny	Lsi02G011620
Gene Ontology terms	GO:0002939 - tRNA N1-guanine methylation (biological process) GO:0005634 - nucleus (cellular component) GO:0000049 - tRNA binding (molecular function) GO:0009019 - tRNA (guanine-N1-)-methyltransferase activity (molecular function)
InterPro domains	IPR007356 - tRNA (guanine-N1-)-methyltransferase, eukaryotic IPR016009 - tRNA methyltransferase TRMD/TRM10-type domain IPR028564 - tRNA methyltransferase TRM10-type domain IPR038459 - tRNA methyltransferase TRM10-type domain superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6583717.1 hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia]

3.6e-117

75.45

Show/hide

Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME+ EEDQSAKMDPP SP  CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKDIE KRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        E+EKKIERLN AKDCG  IVIDLEFS +MTPSEIHSLVQQ                                           EHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYIIGGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT
        DH VD EEN  GDRDDE ETKKTKCDEVSSSNHT
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT

XP_022927615.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata]

3.4e-115

74.25

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME+ EEDQSAKMDPP SP  CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKDIE KRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        E+EKKIERLN AKDCG  IVIDLEFS +MTPSEIHSLVQQ                                           EHRSYIDALQD KENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYIIGGLVDRNRWKGITM K         KLPIGSYLKMSSSQVL VNQV+EILL+Y+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT
        DH VD +EN  GDRDDE ETKKTKCDEVSSSNHT
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT

XP_022973151.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima]

6.9e-116

74.55

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME+IEEDQSAKMDPP SP  CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKDIE KRKEWE+TLASVTEEER+KLIESRRSLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        E+EKKIERLN AKDCG  I+IDLEFS +MTPSEIHSLVQQ                                           EHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYIIGGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT
        DH VD EEN  GDRDDE E+KK KC EVSSSNHT
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT

XP_023520714.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo]

3.6e-117

75.45

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME+IEEDQSAKMDPP SP  CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KE KKKDIE KRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        E+EKKIERLN AKDCG  IVIDLEFS +MTPSEIHSLVQQ                                           EHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYIIGGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT
        DH VD EEN  GDRDDE ETKKTKCDEVSSSNHT
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT

XP_038893739.1 tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida]

4.6e-120

77.15

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME++EEDQSAKMDPP SP A NPPEPPLSKNA+KKLLKQQRYEAKKAEKKLQAKEQKKKDIE KRKEWE+TLASVTEEERSKLIESRRSLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        EREKKIERLN AKDCG NIVIDLEFS++MTPSEIHSLVQQ                                           EHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT DAE VLDDLD KKIYIIGGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQVIEILLKYLETRDWKASFF+VVPQRKRC ADSNS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEEN--GDGDRDDEHETKKTKCDEVSSSNHTL
        DH VDEEEN  GDGD DDEHE KKTKCDEVSSS+ TL
Subjt:  DHQVDEEEN--GDGDRDDEHETKKTKCDEVSSSNHTL

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LYJ3 SAM-dependent MTase TRM10-type domain-containing protein

1.0e-109

71.64

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME++EE  + KMDPP S  ACNPPEPPLSKNARKKLLKQQRYEAKKA+KKLQAKEQKKKDIE KRKEWEE LASVTEEER+KLIESRR+LRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        +REKKIERL+ A+D G NIVIDLEFS++MTP EIHSLVQQ                                           E RSYIDALQ+QKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYI+GGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQVIEILLKYLETRDWK SFFEVVPQRKRC  DSNS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHTL
        DH VD EENGDG  D+++ETKKTKCDEVSSS++ L
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHTL

A0A5D3CW30 tRNA (Guanine(9)-N1)-methyltransferase isoform X1

3.0e-109

72.04

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME +E   S K+DPP SP ACNPPEP LSKNARKKLLKQQRYEAKKA+KKLQAKEQKKKDIE KRKEWEE LA VTEEER+KLIESRR+LRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        +REKKIERLN AKDCG NIVIDLEFS++MTPSEIHSLVQQ                                           E RSYIDALQ+QKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYI+GGLVDRNRWKGIT+KK         +LPIGSYLKMSSSQVL VNQVIEILLKYLETRDWK SFF+VVPQRKRC ADSNS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVS
        DH VD EENGDG  D++ ETKKTKCDEVS
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVS

A0A6J1DYJ8 tRNA (guanine(9)-N(1))-methyltransferase

2.5e-111

71.98

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Query:  TDIWEPTMESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKE
        TD WE  ME++E D  AKMDP S   AC PPE PLSKNARKKL KQQRYEAKKAEKK QAKEQKKKDIE KRKEWEE LASV+EEERSKLIESRRSLRKE
Subjt:  TDIWEPTMESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKE

Query:  RMGKRSEEREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQ
        RMGKRSEEREKK+ERLN AKDCG NIVIDLEFS++MTPSEI+SLVQQ                                           EH+SYI+ L+
Subjt:  RMGKRSEEREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQ

Query:  DQKENLVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKR
        DQKENLVYLT DAE VLDDLDLKKIYIIGGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQV+EILLKYLETRDWKASFFEVVPQRKR
Subjt:  DQKENLVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKR

Query:  CEADSNSDHQVDEEENGDGDRDDEHETKKTKCDEVSSSN
        CEADSNSD  VDEE   +G++D EHETKKTKCDE+SSSN
Subjt:  CEADSNSDHQVDEEENGDGDRDDEHETKKTKCDEVSSSN

A0A6J1EPG7 tRNA (guanine(9)-N(1))-methyltransferase

1.6e-115

74.25

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME+ EEDQSAKMDPP SP  CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKDIE KRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        E+EKKIERLN AKDCG  IVIDLEFS +MTPSEIHSLVQQ                                           EHRSYIDALQD KENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYIIGGLVDRNRWKGITM K         KLPIGSYLKMSSSQVL VNQV+EILL+Y+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT
        DH VD +EN  GDRDDE ETKKTKCDEVSSSNHT
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT

A0A6J1I7W5 tRNA (guanine(9)-N(1))-methyltransferase

3.3e-116

74.55

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Query:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        ME+IEEDQSAKMDPP SP  CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKDIE KRKEWE+TLASVTEEER+KLIESRRSLRKERMGKRSE
Subjt:  MESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV
        E+EKKIERLN AKDCG  I+IDLEFS +MTPSEIHSLVQQ                                           EHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLV

Query:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLT D+E VLDDLDLKKIYIIGGLVDRNRWKGITMKK         KLPIGSYLKMSSSQVL VNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT
        DH VD EEN  GDRDDE E+KK KC EVSSSNHT
Subjt:  DHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHT

SwissProt top hits

e value

%identity

Alignment

O14214 tRNA (guanine(9)-N1)-methyltransferase

2.8e-19

29.57

Show/hide

Query:  EPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGHNIVIDLE
        +P LSK+A K+L +QQ ++A + EK+ + + +KK+               + +EER + IE+   ++           +KK  RL         IV+D  
Subjt:  EPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGHNIVIDLE

Query:  FSYVMTPSEIHSLVQQEHR----------------------------------------------SYIDALQDQKENLVYLTVDAEIVLDDLDLKKIYII
        F  +M   EI+SL QQ  R                                              SY++  + QKE LVYL+ D++  + +LD  KIYII
Subjt:  FSYVMTPSEIHSLVQQEHR----------------------------------------------SYIDALQDQKENLVYLTVDAEIVLDDLDLKKIYII

Query:  GGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNSDHQVDEEENGDGDRDDEHETK
        G +VD+NR+K +   K         KLPI  Y+K++  ++L VNQV EIL  +LE RDW+ +F EV+P+RK     S+    V E+     ++ D    K
Subjt:  GGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNSDHQVDEEENGDGDRDDEHETK

Query:  K
        +
Subjt:  K

Q3MHI8 tRNA methyltransferase 10 homolog A

1.7e-16

28.37

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Query:  ESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEE
        +++ ED      P SS         P+SK   KKL+KQ+++E ++  +K + KE++      KRK+ E      +  + S                    
Subjt:  ESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEE

Query:  REKKIERLNMAKDCGHN---IVIDLEFSYVMTPSEIHSLVQQEHRSYID---ALQD----------------------------------------QKEN
             +R  + +D  H+   ++ID  F  +M   +I  L +Q  R Y +   AL                                          QKE+
Subjt:  REKKIERLNMAKDCGHN---IVIDLEFSYVMTPSEIHSLVQQEHRSYID---ALQD----------------------------------------QKEN

Query:  LVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRK------
        L+YLT D+  +L +LD  K Y+IGGLVD N  KG+T K+         +LP+G+++KM+S +VL VN V EI+L+YLETRDW+ +FF ++PQRK      
Subjt:  LVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRK------

Query:  -------------RCEADSNSDHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHTL
                     R E   NSD     EE    + D  HE +K   D+ +S+  T+
Subjt:  -------------RCEADSNSDHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHTL

Q4KLI2 tRNA methyltransferase 10 homolog A

3.8e-16

29.45

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Query:  ESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEE
        +S EE Q  ++D  +          P+SK   KKL+KQ+ +E ++ ++K + KE++K+                      K +E R  L     G   + 
Subjt:  ESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEE

Query:  REKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQEHRSYIDALQ-----------------------DQ--------------------KENLVY
          + +   N+       ++ID  F  +M   +I  L +Q  R Y +  +                       DQ                    KE+LVY
Subjt:  REKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQEHRSYIDALQ-----------------------DQ--------------------KENLVY

Query:  LTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMK-------KHKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQR-------KR
        LT D+  VL DLD  K Y+IGGLVD N  KG+T K       KH  +LP+  ++KM+S +VL VN V EI+L++LET DW+ +FF ++P R       K 
Subjt:  LTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMK-------KHKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQR-------KR

Query:  CEADSNSDHQV-----DEEENGDGDRDDEHETKKTKCDEVSSS
        CE+ S  DHQ      D    G+  RDD     K +  + SSS
Subjt:  CEADSNSDHQV-----DEEENGDGDRDDEHETKKTKCDEVSSS

Q8C1Z8 tRNA methyltransferase 10 homolog A

3.1e-18

28.86

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Query:  TMESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRS
        T +  EE Q  ++D  +          P+SK   KKL+KQ+++E ++ ++K + KE++K                            R+ L + ++   S
Subjt:  TMESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRS

Query:  EEREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQEHRSYIDALQ-----------------------DQ--------------------KENL
        +  ++K  R ++A+     +VID  F  +M   +I  L +Q  R Y +  +                       DQ                    KE+L
Subjt:  EEREKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQEHRSYIDALQ-----------------------DQ--------------------KENL

Query:  VYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQR-------K
        VYLT D+  VL DLD  K Y+IGGLVD +  KG+T K+         +LP+  ++KM+S +VL VN V EI+L++LETRDW+ +FF ++PQR       K
Subjt:  VYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQR-------K

Query:  RCEADSNSDHQV-----DEEENGDGDRDDEHETKKTKCDEVSS
         CE+ S  DHQ      D    G+  RD+    +K +  + SS
Subjt:  RCEADSNSDHQV-----DEEENGDGDRDDEHETKKTKCDEVSS

Q8TBZ6 tRNA methyltransferase 10 homolog A

8.1e-19

29.31

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Query:  ESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEE
        + I EDQ       S  P       P+SK   KKL+KQ+++E ++  +K + KE++      KRK+ E                     R+ +M   S+ 
Subjt:  ESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEE

Query:  REKKIERLNMAKDCGHN---IVIDLEFSYVMTPSEIHSLVQQEHRSYID---ALQD----------------------------------------QKEN
         ++K     + +D  H+   ++ID  F ++M   +I  L +Q  R Y +   AL                                          +KE+
Subjt:  REKKIERLNMAKDCGHN---IVIDLEFSYVMTPSEIHSLVQQEHRSYID---ALQD----------------------------------------QKEN

Query:  LVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQR-------
        L+YLT D+  +L +LD  K Y+IGGLVD N  KG+T K+      +  +LP+G+++KM+S +VL VN V EI+L+YLETRDW+ +FF ++PQR       
Subjt:  LVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKK------HKTKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQR-------

Query:  KRCEADSNSDHQVDEEENGDGDRDDEHETKKTKCD-----EVSSSNHT
        K CE+ S+ +  V  EE G      E E  + + D     +    NHT
Subjt:  KRCEADSNSDHQVDEEENGDGDRDDEHETKKTKCD-----EVSSSNHT

Arabidopsis top hits

e value

%identity

Alignment

AT5G47680.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

2.6e-73

54.82

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Query:  DQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKI
        D +   D P S PA     PPLSKNA+KK LKQQRYEAKKAEKK Q KE K+K+ E K KEWEETLA+ TEEER KLIESR+SLRKERM KRSEE+EKKI
Subjt:  DQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKI

Query:  ERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLVYLTVDA
        ERLN AK+ G  IV+D++F+++M+ SEI SLVQQ                                           E R YI+A+ DQK+NLVYLT D+
Subjt:  ERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQ-------------------------------------------EHRSYIDALQDQKENLVYLTVDA

Query:  EIVLDDLDLKKIYIIGGLVDRNRWKGITMKKHK------TKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEAD---------
        E VLDDLD K IYIIGGLVDRNR+KGITM K +       KLPIG Y+KMSSSQVL VNQV+EIL+K+LETRDWK +FF V+PQRKR   D         
Subjt:  EIVLDDLDLKKIYIIGGLVDRNRWKGITMKKHK------TKLPIGSYLKMSSSQVLIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEAD---------

Query:  -SNSDHQVDEEENGDGDRDDEHETKKTKCDEV
         S    + D E N   D+ D  E KK  C EV
Subjt:  -SNSDHQVDEEENGDGDRDDEHETKKTKCDEV

Sequences

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CDS sequence

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TGTACTGATATCTGGGAGCCAACAATGGAGTCCATAGAAGAGGACCAGAGCGCTAAAATGGATCCTCCTTCTTCCCCTCCCGCATGCAATCCTCCAGAACCACCCCTCTC
CAAGAATGCTAGGAAGAAGCTACTGAAGCAACAACGATACGAAGCGAAGAAAGCGGAGAAGAAACTTCAGGCAAAAGAGCAGAAGAAGAAAGACATCGAGTGGAAACGGA
AGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGATCCAAGTTGATCGAGTCTCGGAGAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAAGAGAGG
GAGAAGAAGATTGAGAGGCTCAACATGGCCAAAGATTGTGGCCACAATATTGTCATCGACCTTGAATTTTCTTACGTTATGACCCCCTCGGAGATTCACAGTCTTGTTCA
GCAGGAACATAGATCATATATAGATGCTTTGCAAGACCAGAAGGAAAATTTGGTATATCTCACAGTAGACGCAGAAATTGTGCTTGATGACCTTGATCTAAAGAAAATTT
ATATCATTGGTGGGTTAGTTGATCGTAATAGATGGAAAGGGATTACCATGAAGAAACACAAGACAAAACTTCCCATAGGAAGTTACTTGAAGATGTCTAGCTCTCAAGTC
CTTATTGTGAATCAAGTCATAGAGATTCTACTCAAATATTTGGAAACAAGAGATTGGAAGGCTTCTTTCTTTGAGGTTGTTCCTCAAAGAAAAAGATGTGAAGCTGATTC
TAACAGTGACCATCAAGTAGATGAGGAAGAAAATGGAGATGGAGACAGAGATGATGAGCACGAGACGAAGAAGACCAAGTGTGATGAAGTATCTTCCAGTAACCATACTT
TGTAA

mRNA sequence

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Protein sequence

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CTDIWEPTMESIEEDQSAKMDPPSSPPACNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIEWKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEER
EKKIERLNMAKDCGHNIVIDLEFSYVMTPSEIHSLVQQEHRSYIDALQDQKENLVYLTVDAEIVLDDLDLKKIYIIGGLVDRNRWKGITMKKHKTKLPIGSYLKMSSSQV
LIVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNSDHQVDEEENGDGDRDDEHETKKTKCDEVSSSNHTL

Lsi02G011620 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome