CuGenDBv2

Gene ID	Lsi02G025620
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerases II, IV and V subunit 11-like
Genome location	chr02:32104045..32106864
RNA-Seq Expression	Lsi02G025620
Synteny	Lsi02G025620
Gene Ontology terms	GO:0006366 - transcription by RNA polymerase II (biological process) GO:0005665 - RNA polymerase II, core complex (cellular component) GO:0001055 - RNA polymerase II activity (molecular function) GO:0003677 - DNA binding (molecular function) GO:0046983 - protein dimerization activity (molecular function)
InterPro domains	IPR008193 - DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site IPR009025 - DNA-directed RNA polymerase, RBP11-like dimerisation domain IPR036603 - RNA polymerase, RBP11-like subunit IPR037685 - RNA polymerase RBP11

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0058617.1 DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. makuwa]

5.3e-58

92.8

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLSLPLDYLFLQFNWCGV
        LKYELEVMLSLPLD+ F   NW  V
Subjt:  LKYELEVMLSLPLDYLFLQFNWCGV

TYK10426.1 DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. makuwa]

6.7e-61

91.85

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLSLPLDYLF-LQFNWCGVRFQGSRGPI
        LKYELEVMLSLPLD+ F L      VRFQGSRGPI
Subjt:  LKYELEVMLSLPLDYLF-LQFNWCGVRFQGSRGPI

XP_004135939.1 DNA-directed RNA polymerases II, IV and V subunit 11 isoform X3 [Cucumis sativus]

2.8e-51

95.45

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLS
        LKYELE  +S
Subjt:  LKYELEVMLS

XP_008461312.1 PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo]

2.2e-51

95.45

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLS
        LKYELE  +S
Subjt:  LKYELEVMLS

XP_023004699.1 DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 [Cucurbita maxima]

4.8e-51

97.17

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNI+RMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELE
        LKYELE
Subjt:  LKYELE

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CFN3 DNA-directed RNA polymerases II, IV and V subunit 11-like

1.0e-51

95.45

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLS
        LKYELE  +S
Subjt:  LKYELEVMLS

A0A5A7UV57 DNA-directed RNA polymerases II, IV and V subunit 11-like

2.6e-58

92.8

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLSLPLDYLFLQFNWCGV
        LKYELEVMLSLPLD+ F   NW  V
Subjt:  LKYELEVMLSLPLDYLFLQFNWCGV

A0A5D3CJ64 DNA-directed RNA polymerases II, IV and V subunit 11-like

3.2e-61

91.85

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVMLSLPLDYLF-LQFNWCGVRFQGSRGPI
        LKYELEVMLSLPLD+ F L      VRFQGSRGPI
Subjt:  LKYELEVMLSLPLDYLF-LQFNWCGVRFQGSRGPI

A0A6J1H8G6 DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1

2.3e-51

97.17

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNI+RMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELE
        LKYELE
Subjt:  LKYELE

A0A6J1KX24 DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1

2.3e-51

97.17

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNI+RMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDV
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELE
        LKYELE
Subjt:  LKYELE

SwissProt top hits

e value

%identity

Alignment

O08740 DNA-directed RNA polymerase II subunit RPB11

1.2e-23

46.08

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNI++ QL +D  VLFA Y++PHPL+++I++R+QT    SP +A+  A+ DL  EL +
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LK
        L+
Subjt:  LK

P52435 DNA-directed RNA polymerase II subunit RPB11-a

5.2e-24

44.04

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNI++ QL +D  VLFA Y++PHPL+++I++R+QT    SP +A+  A+ DL  EL +
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVML
        L+    V +
Subjt:  LKYELEVML

Q32P79 DNA-directed RNA polymerase II subunit RPB11

5.2e-24

44.04

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNI++ QL +D  VLFA Y++PHPL+++I++R+QT    SP +A+  A+ DL  EL +
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELEVML
        L+    V +
Subjt:  LKYELEVML

Q38859 DNA-directed RNA polymerases II, IV and V subunit 11

1.4e-45

81.13

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA YQLPHPLKY+I+VRI T SQSSPMQAYNQA+NDLDKELD 
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELE
        LK + E
Subjt:  LKYELE

Q86JJ5 DNA-directed RNA polymerase II subunit rpb11

2.3e-24

48.04

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MN PDR+E FV+P+GAKKV+  RDTKI NA+ F + +EDHTIGN++RMQL  D +++FA Y++PHPL++ + +RIQT + ++P++   +++  L +E   
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LK
        L+
Subjt:  LK

Arabidopsis top hits

e value

%identity

Alignment

AT3G52090.1 DNA-directed RNA polymerase, RBP11-like

1.0e-46

81.13

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV
        MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA YQLPHPLKY+I+VRI T SQSSPMQAYNQA+NDLDKELD 
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDV

Query:  LKYELE
        LK + E
Subjt:  LKYELE

AT3G52090.2 DNA-directed RNA polymerase, RBP11-like

5.7e-42

63.24

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Query:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVR--------------------------
        MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA YQLPHPLKY+I+VR                          
Subjt:  MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVR--------------------------

Query:  ----IQTASQSSPMQAYNQAVNDLDKELDVLKYELE
            I T SQSSPMQAYNQA+NDLDKELD LK + E
Subjt:  ----IQTASQSSPMQAYNQAVNDLDKELDVLKYELE

Sequences

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CDS sequence

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ATGAATGTACCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGGTTTCATACGAGAGAGACACGAAGATCATGAATGCGGCGACCTTTATATTAGAGAG
AGAGGATCATACTATTGGAAATATCCTCCGCATGCAACTTCACAGAGATGATAATGTGTTGTTTGCTGCCTACCAGCTTCCTCATCCTCTGAAGTACAGAATACTCGTTC
GGATACAAACTGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTGTCAATGATTTGGACAAGGAGCTTGATGTTTTGAAATATGAGCTTGAGGTAATGCTTTCC
CTCCCCCTTGACTATTTATTTCTGCAATTTAATTGGTGCGGGGTGAGATTTCAAGGTTCTCGAGGACCTATTAGAGTTTGCCTTTCAAGATAA

mRNA sequence

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AAAGAGCCAAACCACGTCGCCTATCTTCATATCGCTATTTCAAGAGAGAGTTTCAGTTGCAGAGAGAAAAAGGAGGAGAAGAAGAAAGACGATGAATGTACCCGATCGCT
ACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGGTTTCATACGAGAGAGACACGAAGATCATGAATGCGGCGACCTTTATATTAGAGAGAGAGGATCATACTATTGGA
AATATCCTCCGCATGCAACTTCACAGAGATGATAATGTGTTGTTTGCTGCCTACCAGCTTCCTCATCCTCTGAAGTACAGAATACTCGTTCGGATACAAACTGCTAGCCA
GTCTTCACCGATGCAGGCATATAATCAGGCTGTCAATGATTTGGACAAGGAGCTTGATGTTTTGAAATATGAGCTTGAGGTAATGCTTTCCCTCCCCCTTGACTATTTAT
TTCTGCAATTTAATTGGTGCGGGGTGAGATTTCAAGGTTCTCGAGGACCTATTAGAGTTTGCCTTTCAAGATAACGAAGGGAAATTTCGAACTCAACAAAGTCGAGTTGG
AAATGGAATGTCAAGAATAAAATTATTGGCCACTTTTCAGTTCGGCTCCTTTTGGTGGGAGTCGATTGTATTCTTTCTTTCTTTTAAATGAAAGTTCAAAAATTTATCCC
AAAAAAAGCCATTTTAATTTATTATTGATTTGAAATAAAATTTAGGCCAAATTATAGAAAATATTTTGAACCTCATTTATAATAACTCACTCTTAAGTTTCATTGAAATT
ATTGTCCTTTAAAAAGAAATTTGAAAAATACCCTTTATTGTGAGTAGTGGGACGATAAATGTTCATCTTTTCTTCCCATCTCTCCAAATTCTTCCTACAGGAAAATTAAA
TTAGAGCTTAATGGCAATCTTCACCACCAGGAGACAATACCAAATGGAAGAGAAACGAAGCCCAAAAAGCGCTGCAAATTGGTGATATATAAGCTATATTCACCACCATG
ATTACACCAAATGGAAGAGATAGAGCTCAAAACAAGGGCTTTCATCCAACGCCATGAAAATAGCTCAAAGATATTAACAGCAGGGATAGATTTAGTATTTGCTCGGGGAC
TGAGTCTTCTTCAACTCTATGCTCTTTATATATATATATATATATATAATTGACATAGTATCAATACCATTAGCTACGTTAGTGATAAATCTGTCTTCTGGCCTCAATTA
GACTCAAGTTCAAGCTCTGCTCTTTTGCATTTTAGAAATTTTTTTAGTACCACGAGAGTATCATATTTTCTTTTAAATTTATCTTAATAAATATTAGTAGACTCTGTATG
AATACAAGCGGATCTATCTCATTTTCAAAGAAAGTTTTCCCATTACCCAG

Protein sequence

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MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLS
LPLDYLFLQFNWCGVRFQGSRGPIRVCLSR

Lsi02G025620 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome