CuGenDBv2

Gene ID	Lsi03G012870
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerase subunit
Genome location	chr03:23686098..23690244
RNA-Seq Expression	Lsi03G012870
Synteny	Lsi03G012870
Gene Ontology terms	GO:0006351 - transcription, DNA-templated (biological process) GO:0042779 - tRNA 3'-trailer cleavage (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR001222 - Zinc finger, TFIIS-type IPR001529 - DNA-directed RNA polymerase, M/15kDa subunit IPR012164 - DNA-directed RNA polymerase subunit/transcription factor S IPR019761 - DNA-directed RNA polymerase M, 15kDa subunit, conserved site IPR029401 - Nudix hydrolase, N-terminal IPR034014 - Pol III subunit C11, C-terminal zinc ribbon

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6597716.1 DNA-directed RNA polymerase III subunit RPC10, partial [Cucurbita argyrosperma subsp. sororia]

3.6e-47

53.43

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNC FGKAAFIQIQLRSADEPA+TFYKCMNEDCRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

KAG7029162.1 DNA-directed RNA polymerase III subunit RPC10, partial [Cucurbita argyrosperma subsp. argyrosperma]

3.6e-47

53.43

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNC FGKAAFIQIQLRSADEPA+TFYKCMNEDCRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

XP_004141147.1 DNA-directed RNA polymerase III subunit RPC10 [Cucumis sativus]

1.6e-47

53.92

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNE+CRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

XP_022932603.1 DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita moschata]

3.6e-47

53.43

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNC FGKAAFIQIQLRSADEPA+TFYKCMNEDCRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

XP_038903069.1 DNA-directed RNA polymerase III subunit RPC10-like [Benincasa hispida]

6.1e-47

53.43

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNE+CR N
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LCM4 DNA-directed RNA polymerase subunit

7.8e-48

53.92

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNE+CRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

A0A1S3CQ50 DNA-directed RNA polymerase subunit

7.8e-48

53.92

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNE+CRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

A0A6J1DGL3 DNA-directed RNA polymerase subunit

1.2e-45

51.47

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYV +LENRVKIKRKQHL+KKELEPIISD+DMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNCAFGKAAFIQ+QLRSADEPATTFYKCMNEDCR N
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

A0A6J1EXG3 DNA-directed RNA polymerase subunit

1.7e-47

53.43

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNC FGKAAFIQIQLRSADEPA+TFYKCMNEDCRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

A0A6J1IB59 DNA-directed RNA polymerase subunit

1.7e-47

53.43

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Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     ATCPNC FGKAAFIQIQLRSADEPA+TFYKCMNEDCRQN
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRED
Subjt:  WRED

SwissProt top hits

e value

%identity

Alignment

O13896 DNA-directed RNA polymerase III subunit RPC10

1.8e-04

46.81

Show/hide

Query:  SSATCPN--CAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED
        +  TC N  C   +A F Q+Q+RSADEP +TFY+C    C+  WRE+
Subjt:  SSATCPN--CAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED

Q04307 DNA-directed RNA polymerase III subunit RPC10

1.7e-07

43.94

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Query:  KHMLDKPGVSWDSLKEGGSSATCPN---CAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED
        K + D  G  WD++ +  +   CPN   C    A F Q+Q+RSADEP TTFYKC+N  C   W+E+
Subjt:  KHMLDKPGVSWDSLKEGGSSATCPN---CAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED

Q2M2S7 DNA-directed RNA polymerase III subunit RPC10

3.8e-07

43.4

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Query:  SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE
        +W+++    ++  CP C   +A F+Q+Q R ADEP TTFYKC N  C   WR+
Subjt:  SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE

Q9CQZ7 DNA-directed RNA polymerase III subunit RPC10

7.6e-08

45.28

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Query:  SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE
        +W+++    ++  CP C   +A F+Q+Q RSADEP TTFYKC N  C   WR+
Subjt:  SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE

Q9Y2Y1 DNA-directed RNA polymerase III subunit RPC10

4.5e-08

45.28

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Query:  SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE
        +W+++    ++ +CP C   +A F+Q+Q RSADEP TTFYKC N  C   WR+
Subjt:  SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE

Arabidopsis top hits

e value

%identity

Alignment

AT1G01210.1 DNA-directed RNA polymerase, subunit M, archaeal

4.0e-20

29.41

Show/hide

Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGN+L++E    G +SRFFC TCPYV+Y++ +V+IK+KQ LVKK +E +++ DD+  AA+TE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     A CP C   KA F  +Q+RSADEP + FY+C+   C   
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRE+
Subjt:  WRED

AT4G07950.1 DNA-directed RNA polymerase, subunit M, archaeal

3.6e-21

30.88

Show/hide

Query:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV
        MEFCPTCGN+L++E    G SSRFFC TCPYV+ +E RV+IK+KQ LVKK +EP+++ DD+  AA+TE                                
Subjt:  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEV

Query:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN
                                                                     A CP C   KA F  +Q+RSADEP + FY+C+   C   
Subjt:  LCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQN

Query:  WRED
        WRE+
Subjt:  WRED

Sequences

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CDS sequence

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ATGGAGTTTTGCCCAACTTGTGGGAACATGCTGCAATTTGAGTTGCCCAATATGGGTAAATCTTCCAGATTCTTCTGCCCAACTTGTCCATATGTGTCCTACTTGGAGAA
CAGGGTAAAAATCAAAAGAAAGCAACATTTGGTAAAAAAGGAATTAGAACCCATAATCTCTGATGACGATATGAAAAATGCAGCTCAAACCGAAGGAGTGAGGCTTCATA
TTCTAGAAATATTGGAGGGAATCATGGATGATATGCACTGGACTGCCCACTCCTCTATTACTATTACCTGTATAGAGGTCCTATGCTTCAAATTACAGCAAAAGTTATGC
TTATGCCGCACGAGCAACCAATTCCCTTGTCTCTCCCTTTCTGGGAAATGGCTGCCTTCACGAGCATGTGATTGGATGCGTGGGGGACTGTGGAAGCATATGTTGGATAA
GCCAGGGGTTTCATGGGACAGTCTAAAAGAGGGAGGGTCGTCCGCAACATGTCCAAATTGCGCGTTTGGCAAGGCTGCTTTTATACAAATACAACTGAGATCAGCTGATG
AACCTGCTACAACATTTTACAAGTGCATGAACGAAGATTGTAGACAGAATTGGCGCGAGGATTAA

mRNA sequence

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Protein sequence

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MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLC
LCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED

Lsi03G012870 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome