CuGenDBv2

Gene ID	Lsi04G000950
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Translation initiation factor IF-2, putative isoform 1
Genome location	chr04:1089996..1095587
RNA-Seq Expression	Lsi04G000950
Synteny	Lsi04G000950
Gene Ontology terms	GO:0006413 - translational initiation (biological process) GO:0003743 - translation initiation factor activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6604007.1 hypothetical protein SDJN03_04616, partial [Cucurbita argyrosperma subsp. sororia]

1.0e-110

89.21

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSD------SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ
        MARRK KK+VKKS PSP R+AKD + N+LKS+      SDEDVERHA AIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSD------SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ

Query:  GEIEVQWKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSI
        GEIEVQWKDT DELHTNPADGIDIHASLL RLSTAYPNCSAGMRSFNGFEFS  SVKTNPFNVENLQIPNF LEEPSDNMVLGMP++LQTPGVSNQRLSI
Subjt:  GEIEVQWKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSI

Query:  GMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        GMTPKTRRLPKPGE+LVSIHGSPLGVY+EDNMEAIHESEEG
Subjt:  GMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

XP_016900279.1 PREDICTED: uncharacterized protein LOC103489436 [Cucumis melo]

2.1e-111

91.06

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARRK KKTVKKS PS GR AKDEA + +K+ SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSIS RG+QGEIEVQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT
        WKDT DELHTNPADG+DIHASLL RLSTAYP CSAGMRSFNGFEFS  SVKTNPFN ENLQIPNFVLEEPSDNMVLGMP+ILQTPG+SNQRLSIGMTPKT
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT

Query:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
Subjt:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

XP_023543921.1 uncharacterized protein LOC111803645 [Cucurbita pepo subsp. pepo]

8.7e-110

88.38

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSD------SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ
        MARRK KK+VKKS PSP R+AKD + N+LKS+      SDEDVERHA AIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSD------SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ

Query:  GEIEVQWKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSI
        GEIEVQWK+T DELHTNPADGIDIHASLL RLSTAYPNCSAGMRSFNGFEFS  SVKTNPFNVENLQIPNF LEEPSDNMVLGMP++LQTPGVSNQRLSI
Subjt:  GEIEVQWKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSI

Query:  GMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        GMTPKTRRLPKPGE+LVSIHGSPLGVY+E+NMEAIHESEEG
Subjt:  GMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

XP_031741813.1 uncharacterized protein LOC101204054 [Cucumis sativus]

5.6e-109

89.17

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARRK KKTVKKS PS GRDAKDEA N LK+DSDEDVERHAAAIRAIRDVEIERLIT LRLLRSYFNKEQLQTPLLQFFEEKLPSLSIS RG+QGEIEV+
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGV-----SNQRLSIG
        WKDT DELHTNPADG+DIHASLL RLSTAYP CSAGMRSFNGFEFS  SVKTNPFN ENLQIPNFVLEEPSD+M LGMP+I+QTPGV     SNQRLSIG
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGV-----SNQRLSIG

Query:  MTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        MTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
Subjt:  MTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

XP_038891412.1 uncharacterized protein LOC120080833 [Benincasa hispida]

2.9e-113

93.16

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARR+ KKTVKKS PSPGRDAKDEA N+LKSD DEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLP+LSISRRGEQGEIEVQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT
        WKDT DELHTNPADGIDIHASLL  LSTAYP CSAGMRSFNGFEFS  SVKTNPFNVENLQIPN  LEEPSDNMVLGMP ILQTPGVSNQRLSIGMTPKT
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT

Query:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEE
        RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEE
Subjt:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEE

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KK81 Uncharacterized protein

1.1e-105

89.36

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARRK KKTVKKS PSP  +AKDEA N      DEDVERHAAAIRAIRDVEIERLIT LRLLRSYFNKEQLQTPLLQFFEEKLP LSISR GEQGEIEVQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT
        WKDT DEL TNPADGIDIHASLL RLS AYPNCSAGMRSFNGFEFS  SVKTNPF VENLQIPNFVLEEPSDN+VLGMP+I QTPGVSNQRLSIGMTPKT
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT

Query:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
Subjt:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

A0A0A0KRH4 Uncharacterized protein

2.7e-109

89.17

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARRK KKTVKKS PS GRDAKDEA N LK+DSDEDVERHAAAIRAIRDVEIERLIT LRLLRSYFNKEQLQTPLLQFFEEKLPSLSIS RG+QGEIEV+
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGV-----SNQRLSIG
        WKDT DELHTNPADG+DIHASLL RLSTAYP CSAGMRSFNGFEFS  SVKTNPFN ENLQIPNFVLEEPSD+M LGMP+I+QTPGV     SNQRLSIG
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGV-----SNQRLSIG

Query:  MTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        MTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
Subjt:  MTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

A0A1S4DWB9 uncharacterized protein LOC103489436

1.0e-111

91.06

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARRK KKTVKKS PS GR AKDEA + +K+ SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSIS RG+QGEIEVQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT
        WKDT DELHTNPADG+DIHASLL RLSTAYP CSAGMRSFNGFEFS  SVKTNPFN ENLQIPNFVLEEPSDNMVLGMP+ILQTPG+SNQRLSIGMTPKT
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT

Query:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
        RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG
Subjt:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

A0A5A7SXV4 Translation initiation factor IF-2, putative isoform 1

4.7e-109

90.87

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        MARRK KKTVKKS PS GR AKDEA + +K+ SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSIS RG+QGEIEVQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT
        WKDT DELHTNPADG+DIHASLL RLSTAYP CSAGMRSFNGFEFS  SVKTNPFN ENLQIPNFVLEEPSDNMVLGMP+ILQTPG+SNQRLSIGMTPKT
Subjt:  WKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT

Query:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIH
        RRLPKPGEMLVSIHGSPLGVYKEDNMEAIH
Subjt:  RRLPKPGEMLVSIHGSPLGVYKEDNMEAIH

A0A6J1ILD3 uncharacterized protein LOC111478496

6.7e-108

87.5

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSD------SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ
        MARRK KK+VKKS PSP R+AKD + N+LKS+      SDEDVERHA AIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFF EKLPSLSISRRGEQ
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSD------SDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQ

Query:  GEIEVQWKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSI
        GEIEVQWKDT DELHTNPADGIDIHASLL RLSTAYPNCSAG+RSFNGFEFS  SVKTNPFNVENLQIPNFVLEEPSDNMVLGMP++LQTPG SNQRLSI
Subjt:  GEIEVQWKDT-DELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSI

Query:  GMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEE
        GMTPKTRRLPKPGE++VSIHGSPLGVY+E NMEAIHESEE
Subjt:  GMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEE

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT4G39630.1 unknown protein

8.3e-50

49.14

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Query:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ
        M +RK K+ VK +      + +       +   DE+VER  AAIRAIRDVEIE+++T LRLLRSYF +EQL TP+L FF+E LP LSISR  E GEIE++
Subjt:  MARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQFFEEKLPSLSISRRGEQGEIEVQ

Query:  WKDTD-ELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT
        W+D + +      +G+D++ S+L+RLS  + +  +   S  G++   N VK N    +N Q+ N V +  S+N +L   +  QTPGV+ QRLS GMTPKT
Subjt:  WKDTD-ELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVSNQRLSIGMTPKT

Query:  RRLPKPGEMLVSIHGSPLGVYKED-NMEAIHE
         RLPK GEM++S+HGSPLGVYKED NM AI+E
Subjt:  RRLPKPGEMLVSIHGSPLGVYKED-NMEAIHE

Sequences

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CDS sequence

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ATATGTGCAAAGAAGTCGGAAGAAGCCGTGACGAAGTGCGGATTCGTCTCAGAATGCTGCAACTGCAGTAAAGGGAAGAAGAAAGATTGGTTGCTGAGAATGGCAAGGCG
AAAGACGAAGAAAACAGTTAAGAAGTCTAGACCTTCACCTGGACGAGACGCAAAAGATGAAGCAGGGAATGACTTAAAGAGTGACTCCGACGAGGATGTCGAACGGCATG
CTGCTGCAATTCGTGCCATTCGGGATGTGGAGATTGAGCGTTTGATTACTGAATTGCGGTTGCTTCGTTCGTATTTCAACAAAGAGCAATTGCAAACTCCTCTTTTGCAA
TTTTTCGAAGAAAAACTTCCAAGCTTGTCCATTTCGAGAAGAGGTGAACAAGGAGAAATTGAAGTACAGTGGAAGGATACAGATGAATTACACACCAATCCAGCTGATGG
AATAGATATACATGCTTCTCTCCTTCAGCGCCTGTCCACAGCTTATCCTAACTGCTCTGCCGGAATGCGATCTTTTAATGGATTTGAATTTTCCTGTAATTCAGTGAAAA
CAAATCCTTTTAATGTTGAGAACCTGCAAATTCCGAACTTCGTCTTGGAGGAGCCCTCGGATAACATGGTGCTTGGCATGCCAAACATTCTCCAAACTCCTGGGGTAAGT
AACCAAAGATTGTCCATTGGGATGACACCAAAAACCCGAAGACTGCCAAAGCCAGGCGAGATGCTTGTGTCTATCCATGGATCCCCCCTTGGTGTATACAAGGAAGACAA
CATGGAAGCAATCCATGAATCAGAAGAGGGTTGA

mRNA sequence

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Protein sequence

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ICAKKSEEAVTKCGFVSECCNCSKGKKKDWLLRMARRKTKKTVKKSRPSPGRDAKDEAGNDLKSDSDEDVERHAAAIRAIRDVEIERLITELRLLRSYFNKEQLQTPLLQ
FFEEKLPSLSISRRGEQGEIEVQWKDTDELHTNPADGIDIHASLLQRLSTAYPNCSAGMRSFNGFEFSCNSVKTNPFNVENLQIPNFVLEEPSDNMVLGMPNILQTPGVS
NQRLSIGMTPKTRRLPKPGEMLVSIHGSPLGVYKEDNMEAIHESEEG

Lsi04G000950 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome