CuGenDBv2

Gene ID	Lsi04G007180
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	tRNA pseudouridine synthase
Genome location	chr04:6573760..6581414
RNA-Seq Expression	Lsi04G007180
Synteny	Lsi04G007180
Gene Ontology terms	GO:0031119 - tRNA pseudouridine synthesis (biological process) GO:0003723 - RNA binding (molecular function) GO:0106029 - tRNA pseudouridine synthase activity (molecular function)
InterPro domains	IPR001406 - Pseudouridine synthase I, TruA IPR020094 - Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal IPR020103 - Pseudouridine synthase, catalytic domain superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_008445970.1 PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo]

3.1e-118

71.65

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLKAVGTGDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

XP_008445972.1 PREDICTED: tRNA pseudouridine synthase A isoform X2 [Cucumis melo]

5.4e-118

73.1

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGD
        VGVLKAVGTGDLTVGD
Subjt:  VGVLKAVGTGDLTVGD

XP_011655540.1 uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus]

1.9e-115

70.73

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTDE+  GDEEEAE MN  PGIQRYLVAVEYIGTRF GSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE E EV  SETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLKAVG+GDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

XP_038877866.1 tRNA pseudouridine synthase A isoform X1 [Benincasa hispida]

4.1e-118

70.59

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE----------------------
        MTEN KHGLEFS DSS QRPLKIGKTTDEVL   EEEAE MNRNPGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                      
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE----------------------

Query:  ---------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDR
                 DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDR
Subjt:  ---------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDR

Query:  AWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-----------------
        AWHVSETLNIGAM                     QANSPIRTLDELDVCEVVSTPYFPTSMER QRQGK EG++EV HSETCE                 
Subjt:  AWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-----------------

Query:  --------VRLMVGVLKAVGTGDLTVGDGSR----KNLGS
                VRLMVGVLKAVGTGDLTVGD  R    KN+ S
Subjt:  --------VRLMVGVLKAVGTGDLTVGDGSR----KNLGS

XP_038877867.1 tRNA pseudouridine synthase A isoform X2 [Benincasa hispida]

1.7e-119

73.17

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLEFS DSS QRPLKIGKTTDEVL   EEEAE MNRNPGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDELDVCEVVSTPYFPTSMER QRQGK EG++EV HSETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLKAVGTGDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KRX0 tRNA pseudouridine synthase

9.2e-116

70.73

Show/hide

Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTDE+  GDEEEAE MN  PGIQRYLVAVEYIGTRF GSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE E EV  SETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLKAVG+GDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

A0A1S3BDG9 tRNA pseudouridine synthase

1.5e-118

71.65

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLKAVGTGDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

A0A1S3BDY4 tRNA pseudouridine synthase

2.6e-118

73.1

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGD
        VGVLKAVGTGDLTVGD
Subjt:  VGVLKAVGTGDLTVGD

A0A5D3CZZ6 tRNA pseudouridine synthase

1.5e-118

71.65

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KHGLE S DSSSQRPLKIGKTTD+V  GDEEEAE MN  PGIQRYLVAVEYIGTRFSGSQ+QP CRTVVG LE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVVH+AVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHV ETLNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSPIRTLDEL+VCEV STPYFPTSMER QRQGKEE ENE  HSETCE                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLKAVGTGDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

A0A6J1KFZ8 tRNA pseudouridine synthase

2.5e-113

69.21

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Query:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG
        MTEN KH LEFS DSS QRPLKIGKTT+E L GD+EEAE MNRNPGIQRYLVAVEYIGTRFSGSQ+QP CRTVVGVLE                   DAG
Subjt:  MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAG

Query:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA
        VHALSNVCHVDVERISKRKPGEVLPPHEPGVV RAVNHFLQK++GDVTVVDVRSVPND+HARYKAQERTYFYRLVSGPE LSSF+KDR WHV E LNIGA
Subjt:  VHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGA

Query:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM
        M                     QANSP+RTLDELDVCEV+STPYFP+SMER QRQGKEEGENEV HSE+ E                         VRLM
Subjt:  M---------------------QANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCE-------------------------VRLM

Query:  VGVLKAVGTGDLTVGDGSR----KNLGS
        VGVLK VGTGDLTVGD  R    KN+ S
Subjt:  VGVLKAVGTGDLTVGDGSR----KNLGS

SwissProt top hits

e value

%identity

Alignment

A9ILJ7 tRNA pseudouridine synthase A

1.2e-14

27.38

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Query:  IQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGD
        + R+ + +EY G+ ++G Q+Q    T+ G LE                   DAGVHA   V HVD  +          PPH    V  A+N  L++    
Subjt:  IQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGD

Query:  VTVVDVRSVPNDFHARYKAQERTYFYRLVS--GPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPY
        +++++V +VP+DF AR+ A +R Y +++++   P AL++    R W + + LN  AM                     QA SPIRTL+ LD+        
Subjt:  VTVVDVRSVPNDFHARYKAQERTYFYRLVS--GPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPY

Query:  FPTSMERNQRQGKE-----EGENEVSHSETCEVRLMVGVLKAVGTGDLTVGD
                Q++G+E     +  + + H    ++R   G L  VG G  T  D
Subjt:  FPTSMERNQRQGKE-----EGENEVSHSETCEVRLMVGVLKAVGTGDLTVGD

Q6G547 tRNA pseudouridine synthase A

8.9e-15

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Query:  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVT
        R+ + +EY G+ ++G Q+Q   RT+   LE                   DAGVHA   V HVD E+  +              V  A+N  LQK   ++ 
Subjt:  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVT

Query:  VVDVRSVPNDFHARYKAQERTYFYRLVS--GPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPYFP
        ++ V++VP+ F AR+ A +R Y +++++   P AL++    R W + + LN  AM                     QA SPIRTL+ LDV          
Subjt:  VVDVRSVPNDFHARYKAQERTYFYRLVS--GPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPYFP

Query:  TSMERNQRQGKE-----EGENEVSHSETCEVRLMVGVLKAVGTGDLTVGD
              QR+G+E     +  + + H    ++R   G L  VG G  T  D
Subjt:  TSMERNQRQGKE-----EGENEVSHSETCEVRLMVGVLKAVGTGDLTVGD

Q6GQ53 tRNA pseudouridine synthase-like 1

1.8e-15

28.57

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Query:  RYLVAVEYIGTRFSGSQKQPTCRTVVGV---LE--------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHE
        RYLV  +Y GT++SG  + P  ++V+GV   LE                    D+GVHA+ N+ HVD+ER   +      PP    ++ +A+NH LQ   
Subjt:  RYLVAVEYIGTRFSGSQKQPTCRTVVGV---LE--------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHE

Query:  GDVTVVDVRSVPNDFHARYKAQERTYFYRLVSG---PEALSSFEKDRAWHVSETLNIGAMQA-----------------------NSPIRTLDELDV---
          + ++    V ++FHAR+ A  RTY YR+ +G   PE L  FE +  W + + LN+ A+                          SPI+TL + D+   
Subjt:  GDVTVVDVRSVPNDFHARYKAQERTYFYRLVSG---PEALSSFEKDRAWHVSETLNIGAMQA-----------------------NSPIRTLDELDV---

Query:  CEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMVGVLKAVGTGDLT
        C +++  +   +++        +           +VR M G L AVG G LT
Subjt:  CEVVSTPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMVGVLKAVGTGDLT

Q8N0Z8 tRNA pseudouridine synthase-like 1

4.3e-17

30.71

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Query:  RYLVAVEYIGTRFSGSQKQPTCRTVVGV---LE--------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHE
        RYLV  +Y+GT F+G       +  VGV   LE                    DAGVHALSN  H+DV+R S R      PP  P V+  A+N  L +H 
Subjt:  RYLVAVEYIGTRFSGSQKQPTCRTVVGV---LE--------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHE

Query:  GDVTVVDVRSVPNDFHARYKAQERTYFYRLVSG---PEALSSFEKDRAWHV-SETLNIGAMQ---------------------ANSPIRTLDELDVCEVV
          + V+    VP+DFHAR+ A  RTY YRL +G    + L  FE++  W + ++ L++ AMQ                       SP+RTL  + V    
Subjt:  GDVTVVDVRSVPNDFHARYKAQERTYFYRLVSG---PEALSSFEKDRAWHV-SETLNIGAMQ---------------------ANSPIRTLDELDVCEVV

Query:  STPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMVGVLKAVGTGDLTVGD-----GSRKNLGSEECEFSQSNGSCMRTL
        ++P       R  R    E E++       +VR M  VL AVG G L          S+  LG  +   + ++G  ++++
Subjt:  STPYFPTSMERNQRQGKEEGENEVSHSETCEVRLMVGVLKAVGTGDLTVGD-----GSRKNLGSEECEFSQSNGSCMRTL

Q9ZCA3 tRNA pseudouridine synthase A

1.8e-15

29.86

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Query:  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVT
        RY + +EY+GT F+G Q+Q    +V  +LE                   DAGVHA+  V H D+ +             EP  + +A+N+F++ +  DV 
Subjt:  RYLVAVEYIGTRFSGSQKQPTCRTVVGVLE-------------------DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVT

Query:  VVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCE-------VVS
        V +   V N+FHAR+ A  R Y YR+++     S  + +RAW +S  L+I AM                     Q+ SPI+TL E+++ +        +S
Subjt:  VVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGAM---------------------QANSPIRTLDELDVCE-------VVS

Query:  TPYFPTSMERN
         P F   M RN
Subjt:  TPYFPTSMERN

Arabidopsis top hits

e value

%identity

Alignment

AT1G09800.1 Pseudouridine synthase family protein

2.2e-69

47.81

Show/hide

Query:  MTENHKHGLE-FSFDSSSQRPLKIGKTTDEVLRGDEE-----EAEPMNR-NPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE---------------
        M++N KH L+  + + S +R  K+ +         EE     + E MN  NP +QRYLVA+EYIGTRFSGSQ+Q   RTVVGVL+               
Subjt:  MTENHKHGLE-FSFDSSSQRPLKIGKTTDEVLRGDEE-----EAEPMNR-NPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLE---------------

Query:  ----DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVS
            DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVV RAVNHFLQ+ +GDV V+DVR VP+++HARYKAQERTYFYRL+SG + LS  EKDR WHV 
Subjt:  ----DAGVHALSNVCHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVS

Query:  ETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPYFPTSMER--------------NQRQ----------GKEEGENEVS----
        E LN+  M                     QA SP+R+LDEL++ EV STPYFP+  ER              NQ +          G+ EG  E      
Subjt:  ETLNIGAM---------------------QANSPIRTLDELDVCEVVSTPYFPTSMER--------------NQRQ----------GKEEGENEVS----

Query:  -HSETC-------------EVRLMVGVLKAVGTGDLTVGDGSR
             C             +VRL+VG LK VGTG+LTV D  R
Subjt:  -HSETC-------------EVRLMVGVLKAVGTGDLTVGDGSR

Sequences

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CDS sequence

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ATGACGGAAAACCATAAGCATGGACTAGAATTCAGTTTCGATTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAAGTTCTCCGCGGCGACGAGGAGGA
AGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTCGTCGCAGTCGAGTATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGG
TTGGCGTGCTTGAGGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATTAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCG
GGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTCCATGCTAGATATAAAGCTCA
AGAGCGTACATACTTTTATCGGTTAGTTTCTGGGCCGGAAGCTCTGTCAAGCTTCGAGAAGGATCGAGCATGGCACGTGTCTGAGACACTAAATATTGGGGCAATGCAGG
CAAACTCACCAATCAGAACTCTAGATGAACTAGACGTTTGTGAGGTGGTTTCAACGCCTTATTTTCCAACATCAATGGAGCGAAATCAAAGGCAGGGCAAAGAAGAAGGT
GAGAATGAAGTGTCACATTCAGAAACTTGTGAGGTGAGACTGATGGTTGGAGTTCTTAAAGCGGTCGGCACCGGAGATCTAACAGTAGGAGATGGTAGTAGAAAAAATCT
TGGAAGCGAAGAATGTGAGTTCAGCCAGTCCAATGGCTCCTGCATGCGGACTCTATCTCGGACATGTCAAATATGA

mRNA sequence

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GCAAAGCAAACTCCCATCGTCTACCTCGGGCAGTGACGAGGAGTGGCGAAAATCAATGACGGAAAACCATAAGCATGGACTAGAATTCAGTTTCGATTCATCATCTCAAA
GGCCCTTGAAAATTGGAAAGACCACAGACGAAGTTCTCCGCGGCGACGAGGAGGAAGCAGAACCCATGAACCGTAACCCTGGAATTCAACGCTACCTCGTCGCAGTCGAG
TATATAGGAACTCGTTTTTCTGGGTCTCAGAAGCAGCCGACCTGCCGTACGGTGGTTGGCGTGCTTGAGGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGA
TGTTGAGCGCATTAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAGGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTA
CTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTCCATGCTAGATATAAAGCTCAAGAGCGTACATACTTTTATCGGTTAGTTTCTGGGCCGGAAGCTCTGTCAAGCTTC
GAGAAGGATCGAGCATGGCACGTGTCTGAGACACTAAATATTGGGGCAATGCAGGCAAACTCACCAATCAGAACTCTAGATGAACTAGACGTTTGTGAGGTGGTTTCAAC
GCCTTATTTTCCAACATCAATGGAGCGAAATCAAAGGCAGGGCAAAGAAGAAGGTGAGAATGAAGTGTCACATTCAGAAACTTGTGAGGTGAGACTGATGGTTGGAGTTC
TTAAAGCGGTCGGCACCGGAGATCTAACAGTAGGAGATGGTAGTAGAAAAAATCTTGGAAGCGAAGAATGTGAGTTCAGCCAGTCCAATGGCTCCTGCATGCGGACTCTA
TCTCGGACATGTCAAATATGATCTACCGCCAGCTCCCCACTGAATGAAACCTCCATTTCTTTCTTCACTTCCGAGGATTAAAATACGGCTGTACTGGCAATTTCCCTCTA
GTATCACGTTTTGAGAATGTCCTATATCCAGACTGTTTCATTGCAACAAAGTAACTCCAGCACTTCCCTCATCAACTAACCATGAACATAAGGTTGCAGAATCATACCCA
TGTCGCTTCTTCGATTTCTGTTTTGACTGACCTTTCAGAGCTTTCACCTGACTGGAT

Protein sequence

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MTENHKHGLEFSFDSSSQRPLKIGKTTDEVLRGDEEEAEPMNRNPGIQRYLVAVEYIGTRFSGSQKQPTCRTVVGVLEDAGVHALSNVCHVDVERISKRKPGEVLPPHEP
GVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEALSSFEKDRAWHVSETLNIGAMQANSPIRTLDELDVCEVVSTPYFPTSMERNQRQGKEEG
ENEVSHSETCEVRLMVGVLKAVGTGDLTVGDGSRKNLGSEECEFSQSNGSCMRTLSRTCQI

Lsi04G007180 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome