CuGenDBv2

Gene ID	Lsi04G009680
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease H
Genome location	chr04:10147637..10148005
RNA-Seq Expression	Lsi04G009680
Synteny	Lsi04G009680
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006310 - DNA recombination (biological process) GO:0015074 - DNA integration (biological process) GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic (biological process) GO:0005634 - nucleus (cellular component) GO:0030430 - host cell cytoplasm (cellular component) GO:0003723 - RNA binding (molecular function) GO:0003887 - DNA-directed DNA polymerase activity (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0033845.1 uncharacterized protein E6C27_scaffold43059G00190 [Cucumis melo var. makuwa]

2.1e-42

73.77

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MK EF DE +M V   +  QD E WTM FDGATNEVGHGVGAIL S + K YPLT KLYFDCT+NMA YEACSMGVQMA DMKIK LQV+ D LL +HQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
        NGE ET DSKLIPY+KYIRELA
Subjt:  NGECETRDSKLIPYNKYIRELA

KAA0046609.1 hypothetical protein E6C27_scaffold114G001550 [Cucumis melo var. makuwa]

9.4e-43

71.31

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKF+F DE IM +   ++     TWTM+FDGATNE+GHGVGAIL+SP+ K YPLTAKLYFDCTNNMA YEACSMGV+MA +MK+K L+V GD LLVVHQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
        NGE ETRD+KLIPYN YIR +A
Subjt:  NGECETRDSKLIPYNKYIRELA

KAA0049671.1 uncharacterized protein E6C27_scaffold76G00060 [Cucumis melo var. makuwa]

2.7e-42

71.31

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKF+F DE IM +  +N +  P TWTM+FDGATNE+GHGVG IL+SP+ K YPLTAKLYFDCTNNMA YEACSMGV+MA +MKIK L+V GD +LVVHQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
        NGE ETRD+KLIPYN YI  +A
Subjt:  NGECETRDSKLIPYNKYIRELA

XP_016899404.1 PREDICTED: uncharacterized protein LOC107990505 [Cucumis melo]

9.4e-43

75.42

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKFEF DE +MTV   +  QD E WTM FDGATNEVGHGVGAIL  P+ K YPLTAKLYFDCTNNMA YE CSMGV+MA D+KIK LQV+ D LLV+HQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYI
        N E ETRDSKLIPYNKYI
Subjt:  NGECETRDSKLIPYNKYI

XP_022143495.1 LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia]

4.6e-42

66.39

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        +KF+F DEYI T+     + DP+TWTM+FDGA+NE+GHG+GAILISP+ +LYPLTA+L FDCT+NMA YEACSMGVQ A DMK+K L+VFGD +LV+HQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
         GE ETRD KL+PY + I EL+
Subjt:  NGECETRDSKLIPYNKYIRELA

TrEMBL top hits

e value

%identity

Alignment

A0A1S4DTT9 uncharacterized protein LOC107990505

4.5e-43

75.42

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKFEF DE +MTV   +  QD E WTM FDGATNEVGHGVGAIL  P+ K YPLTAKLYFDCTNNMA YE CSMGV+MA D+KIK LQV+ D LLV+HQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYI
        N E ETRDSKLIPYNKYI
Subjt:  NGECETRDSKLIPYNKYI

A0A5A7TZF3 Ribonuclease H

4.5e-43

71.31

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKF+F DE IM +   ++     TWTM+FDGATNE+GHGVGAIL+SP+ K YPLTAKLYFDCTNNMA YEACSMGV+MA +MK+K L+V GD LLVVHQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
        NGE ETRD+KLIPYN YIR +A
Subjt:  NGECETRDSKLIPYNKYIRELA

A0A5A7U7X5 Ribonuclease H

1.3e-42

71.31

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKF+F DE IM +  +N +  P TWTM+FDGATNE+GHGVG IL+SP+ K YPLTAKLYFDCTNNMA YEACSMGV+MA +MKIK L+V GD +LVVHQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
        NGE ETRD+KLIPYN YI  +A
Subjt:  NGECETRDSKLIPYNKYIRELA

A0A5A7UKV4 Retrovirus-related Pol polyprotein from transposon 17.6

4.5e-43

75.42

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MKFEF DE +MTV   +  QD E WTM FDGATNEVGHGVGAIL  P+ K YPLTAKLYFDCTNNMA YE CSMGV+MA D+KIK LQV+ D LLV+HQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYI
        N E ETRDSKLIPYNKYI
Subjt:  NGECETRDSKLIPYNKYI

A0A5D3BPI8 RNase H domain-containing protein

1.0e-42

73.77

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Query:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL
        MK EF DE +M V   +  QD E WTM FDGATNEVGHGVGAIL S + K YPLT KLYFDCT+NMA YEACSMGVQMA DMKIK LQV+ D LL +HQL
Subjt:  MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQL

Query:  NGECETRDSKLIPYNKYIRELA
        NGE ET DSKLIPY+KYIRELA
Subjt:  NGECETRDSKLIPYNKYIRELA

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT3G01410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.6e-08

32.67

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Query:  ETWTMLFDGAT--NEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQLNGECETRDSKLIPYNKYIRE
        ++ T+ FDGA+  N    G GA+L + +  +     +   + TNN+A Y A  +G++ A D   KN+ V GD +LV  Q+ G  +T   K+    K  +E
Subjt:  ETWTMLFDGAT--NEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQLNGECETRDSKLIPYNKYIRE

Query:  L
        L
Subjt:  L

AT3G01410.2 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.6e-08

32.67

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Query:  ETWTMLFDGAT--NEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQLNGECETRDSKLIPYNKYIRE
        ++ T+ FDGA+  N    G GA+L + +  +     +   + TNN+A Y A  +G++ A D   KN+ V GD +LV  Q+ G  +T   K+    K  +E
Subjt:  ETWTMLFDGAT--NEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQLNGECETRDSKLIPYNKYIRE

Query:  L
        L
Subjt:  L

Sequences

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CDS sequence

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ATGAAATTTGAATTTACTGATGAATACATCATGACGGTCCCCACATTGAATGCGGCACAAGATCCTGAAACGTGGACAATGTTGTTTGATGGAGCCACAAATGAAGTGGG
GCACGGTGTAGGAGCTATTTTGATATCTCCTGAGAGGAAGTTGTATCCCCTAACTGCTAAGCTATACTTTGACTGCACGAATAACATGGCTGTGTATGAAGCATGCAGTA
TGGGAGTTCAGATGGCTAATGACATGAAGATTAAAAACTTGCAAGTTTTTGGAGATCCTCTTTTGGTAGTACATCAACTAAACGGGGAGTGCGAGACAAGAGACTCTAAG
TTGATTCCCTATAATAAGTATATCCGAGAGTTGGCCTAA

mRNA sequence

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Protein sequence

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MKFEFTDEYIMTVPTLNAAQDPETWTMLFDGATNEVGHGVGAILISPERKLYPLTAKLYFDCTNNMAVYEACSMGVQMANDMKIKNLQVFGDPLLVVHQLNGECETRDSK
LIPYNKYIRELA

Lsi04G009680 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome