CuGenDBv2

Gene ID	Lsi04G023670
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Nitrate reductase
Genome location	chr04:30814473..30814769
RNA-Seq Expression	Lsi04G023670
Synteny	Lsi04G023670
Gene Ontology terms	GO:0006809 - nitric oxide biosynthetic process (biological process) GO:0042128 - nitrate assimilation (biological process) GO:0009703 - nitrate reductase (NADH) activity (molecular function) GO:0020037 - heme binding (molecular function) GO:0030151 - molybdenum ion binding (molecular function) GO:0043546 - molybdopterin cofactor binding (molecular function) GO:0050464 - nitrate reductase (NADPH) activity (molecular function) GO:0071949 - FAD binding (molecular function)
InterPro domains	IPR001433 - Oxidoreductase FAD/NAD(P)-binding IPR039261 - Ferredoxin-NADP reductase (FNR), nucleotide-binding domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAE8649633.1 hypothetical protein Csa_012045 [Cucumis sativus]

2.5e-30

72.73

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G  +     EHLPE S  T+ALA GP  MIKF V+P+LEKMNYDTTNS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

KAG7024645.1 hypothetical protein SDJN02_13463, partial [Cucurbita argyrosperma subsp. argyrosperma]

4.0e-28

67.68

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI +++ELDEWS++DKRF VWYVV ESVREGWE+S G        EHLP  S ++LALA GP  MIKF VQPNLEKMNYDT +S LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

XP_008444121.1 PREDICTED: nitrate reductase [NADH]-like [Cucumis melo]

1.1e-30

73.74

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G        EHLPE S  T+ALA GP  MIKF VQPNLEKMNYDT NS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

XP_011652901.1 uncharacterized protein LOC101216001 isoform X1 [Cucumis sativus]

2.5e-30

72.73

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G  +     EHLPE S  T+ALA GP  MIKF V+P+LEKMNYDTTNS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

XP_038897076.1 nitrate reductase [NADH]-like [Benincasa hispida]

2.5e-30

72.73

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR E+DI LK+ELDEW+K+DKRFKVWYVV+ESVREGW++S G        EHLPE S  TLALA GP  MIKF VQPNLEKMNYDTTNS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L1Y7 Nitrate reductase

1.2e-30

72.73

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G  +     EHLPE S  T+ALA GP  MIKF V+P+LEKMNYDTTNS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

A0A1S3BAF3 Nitrate reductase

5.4e-31

73.74

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G        EHLPE S  T+ALA GP  MIKF VQPNLEKMNYDT NS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

A0A5A7VEG1 Nitrate reductase

5.4e-31

73.74

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G        EHLPE S  T+ALA GP  MIKF VQPNLEKMNYDT NS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

A0A6J1C1V3 nitrate reductase [NADH]-like

1.9e-28

68.69

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR E+DI L++E+DEW K+DKRFKVWYVV ESVREGWEFS G        EHLP+ S +TLALA GP  MIKF VQPNLEKMNYDT +S L+F
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

A0A6J1F6X9 Nitrate reductase

5.6e-28

67.68

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MFLVY NR ENDI ++ ELDEWS++DKRF VWYVV ESVREGWE+S G        EHLP  S + LALA GP  MIKF VQPNLEKMNYDT +S LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

SwissProt top hits

e value

%identity

Alignment

P17569 Nitrate reductase [NADH]

2.1e-27

60.61

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        M++VY NR E+DI L+ ELD W+K ++R KVWYVV ES+REGWE+S G    +   EH+P  + +TLALA GP +MI+F VQPNLEKMNYDT NS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

P27783 Nitrate reductase [NAD(P)H]

1.6e-24

56.57

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        MF+VY NR E+DI L++ELD+W+K  ++ KVWYVV ES REGWE+S G  +     EH+PE S + LALA G  SMI+  V+ NLEKMNYDT NS ++F
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

P39870 Inducible nitrate reductase [NADH] 2

5.6e-25

57.58

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        M +VY NR E+DI LK+ELDEW+K   R KVWYV+  S+REGWE+S G        EH+P  S +TLAL  GP  MI+F VQPNLEK+ YDT N+ LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

P43101 Nitrate reductase [NADH]

6.2e-24

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Query:  MFLVYTNRMENDIFLKKELDEWS-KNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        M++VY NR E+DI L++ELD W+ K   R KVWYVV++S+REGW++S G    D   EH+PE S +TLALA GP  MI+F + PNLEKM YD  NS LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWS-KNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

P54233 Inducible nitrate reductase [NADH] 1

1.1e-25

58.59

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Query:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
        M +VY NR E+DI LK+ELDEW+K   R KVWYV+ ES+REGWE+S G        EH+P  S +TLAL  GP  MI+F VQPNLEK+ YDT N+ LVF
Subjt:  MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

Arabidopsis top hits

e value

%identity

Alignment

AT1G37130.1 nitrate reductase 2

3.6e-19

48.51

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Query:  MFLVYTNRMENDIFLKKELDEWSKN-DKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPED-SGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLV
        M+++Y NR E DI L++ELD W++    R KVWYVV ES +EGW +S G        EH+P+   G+ LA+A GP  MI+F VQPNLEKM Y+    +L+
Subjt:  MFLVYTNRMENDIFLKKELDEWSKN-DKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPED-SGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLV

Query:  F
        F
Subjt:  F

AT1G77760.1 nitrate reductase 1

8.1e-19

48.51

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Query:  MFLVYTNRMENDIFLKKELDEW-SKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPED-SGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLV
        M++VY NR E+DI +++EL+ W SK+ +R K+WYVV E  +EGW +S G        EH+PE   G +LALA GP  MI+F +QPNLEKM Y+     L+
Subjt:  MFLVYTNRMENDIFLKKELDEW-SKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPED-SGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLV

Query:  F
        F
Subjt:  F

Sequences

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CDS sequence

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ATGTTTCTGGTATATACGAACAGGATGGAGAATGATATTTTTTTGAAGAAAGAGTTAGATGAGTGGAGTAAGAACGATAAGAGGTTTAAAGTATGGTATGTTGTAAGTGA
GAGTGTAAGGGAAGGGTGGGAATTCAGTGGGAGTGATAAGGGAGACTATCATGGGGAACATCTTCCAGAAGATTCGGGGAATACGTTGGCCTTGGCTTATGGACCTCTGT
CGATGATTAAGTTTGTTGTGCAACCCAACTTGGAGAAGATGAACTACGACACTACTAATTCGTGGTTGGTGTTTTAG

mRNA sequence

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Protein sequence

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MFLVYTNRMENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFSGSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF

Lsi04G023670 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome