CuGenDBv2

Gene ID	Lsi05G005970
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
Genome location	chr05:7400728..7403990
RNA-Seq Expression	Lsi05G005970
Synteny	Lsi05G005970
Gene Ontology terms	GO:0032981 - mitochondrial respiratory chain complex I assembly (biological process) GO:0005739 - mitochondrion (cellular component) GO:0016020 - membrane (cellular component)
InterPro domains	IPR007763 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0033972.1 NDUFA12 domain-containing protein [Cucumis melo var. makuwa]

7.6e-56

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMERNSKEG LRK+I+T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
        GQV GP+LKSFIRQ PTGSEGAQDVEGL TRD+LR               SSEPVGSG TFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

XP_004152928.1 uncharacterized protein LOC101214441 isoform X2 [Cucumis sativus]

9.9e-56

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWV+FKGEDDPTSIPVEWICWLNGQRKKAPTPEEM ELEARRERVRQNVA                       +LKKEEMERNSKEGNLRK+I+T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
        GQV GP+LKSFIRQ PTGSEGAQDVEGL TRDSLR               SSEPVGSG TF+PGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

XP_038903173.1 uncharacterized protein LOC120089839 isoform X1 [Benincasa hispida]

9.6e-59

70.62

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMERNSKEGNLRK+I+T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSL----------------------RSSEPVGSGQTFRPGTWQPPT
        GQVAGP+LKSFIRQFPTGSEGAQDVEGLTTRDSL                      RSSEPVGSGQTFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSL----------------------RSSEPVGSGQTFRPGTWQPPT

XP_038903174.1 uncharacterized protein LOC120089839 isoform X2 [Benincasa hispida]

1.5e-59

73.53

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMERNSKEGNLRK+I+T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
        GQVAGP+LKSFIRQFPTGSEGAQDVEGLTTRDSLR               SSEPVGSGQTFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

XP_038903176.1 uncharacterized protein LOC120089839 isoform X4 [Benincasa hispida]

1.3e-58

71.02

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Query:  VKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINTG
        +KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMERNSKEGNLRK+I+TG
Subjt:  VKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINTG

Query:  QVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSL----------------------RSSEPVGSGQTFRPGTWQPPT
        QVAGP+LKSFIRQFPTGSEGAQDVEGLTTRDSL                      RSSEPVGSGQTFRPGTWQPPT
Subjt:  QVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSL----------------------RSSEPVGSGQTFRPGTWQPPT

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L526 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

4.8e-56

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWV+FKGEDDPTSIPVEWICWLNGQRKKAPTPEEM ELEARRERVRQNVA                       +LKKEEMERNSKEGNLRK+I+T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
        GQV GP+LKSFIRQ PTGSEGAQDVEGL TRDSLR               SSEPVGSG TF+PGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

A0A5A7SS01 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

3.7e-56

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMERNSKEG LRK+I+T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
        GQV GP+LKSFIRQ PTGSEGAQDVEGL TRD+LR               SSEPVGSG TFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

A0A6J1DNE9 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

3.8e-53

67.65

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        L+KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNV                        +LKK+EMERNS+EGNLRKVI+ 
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
         QVAGP+L SFIRQFPTGSEGAQD EG   R+S R               SSEPVGSGQTFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

A0A6J1FNU8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

5.0e-53

68.24

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMER SKE NLRKV++T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
          VAGPNLKSFIRQFPTGSEGAQD EG   +DSLR               SSEP+GSGQTFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

A0A6J1JVV8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

5.0e-53

68.24

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Query:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT
        ++KEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEM+ELEARRERVRQNVA                       +LKKEEMER SKE NLRKV++T
Subjt:  LVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINT

Query:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT
          VAGPNLKSFIRQFPTGSEGAQD EG   +DSLR               SSEP+GSGQTFRPGTWQPPT
Subjt:  GQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR---------------SSEPVGSGQTFRPGTWQPPT

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT4G26965.1 NADH:ubiquinone oxidoreductase, 17.2kDa subunit

6.9e-39

48.89

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Query:  TQLSLIGFLVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEG
        +++  I   VKEKRWV F+ E+DPTSIPVEWICWLNGQRK+APTPEEMIELEARRERV+ NVA                       +LKKEE E+ ++ G
Subjt:  TQLSLIGFLVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEG

Query:  NLRKVINTGQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR-----------------SSEPVGSGQTFRPGTWQPPT
          RK I  G+V GP+L SF+RQFP  S+G +  E     D  R                 ++EP GSG +FRPGTWQPP+
Subjt:  NLRKVINTGQVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR-----------------SSEPVGSGQTFRPGTWQPPT

AT4G26965.2 NADH:ubiquinone oxidoreductase, 17.2kDa subunit

3.4e-38

50.29

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Query:  VKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINTG
        +KEKRWV F+ E+DPTSIPVEWICWLNGQRK+APTPEEMIELEARRERV+ NVA                       +LKKEE E+ ++ G  RK I  G
Subjt:  VKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINTG

Query:  QVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR-----------------SSEPVGSGQTFRPGTWQPPT
        +V GP+L SF+RQFP  S+G +  E     D  R                 ++EP GSG +FRPGTWQPP+
Subjt:  QVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLR-----------------SSEPVGSGQTFRPGTWQPPT

Sequences

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CDS sequence

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CTCACACAACTTTCACTCATTGGTTTTCTAGTGAAAGAGAAGAGATGGGTGGTATTTAAGGGTGAGGATGATCCAACGTCAATTCCAGTGGAATGGATATGTTGGTTAAA
TGGGCAGCGTAAAAAGGCTCCAACCCCAGAGGAAATGATTGAGCTAGAAGCTAGGCGTGAACGTGTACGACAAAATGTTGCTCAAGAACCAATATCATTCTCATTCTTTG
ACTTAACATGGTCTTATTGGAAGTTATCTGTGATCAATTTAGTTCTCAAGAAAGAAGAGATGGAAAGAAACTCAAAAGAAGGCAATTTACGGAAAGTCATCAACACTGGT
CAAGTTGCAGGTCCCAACTTGAAAAGCTTTATTCGACAGTTCCCAACAGGTTCAGAAGGTGCCCAAGATGTGGAAGGATTGACTACAAGGGATAGCCTGAGGTCCTCCGA
GCCCGTTGGGTCTGGCCAAACATTTAGACCAGGAACATGGCAGCCACCAACATGA

mRNA sequence

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Protein sequence

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LTQLSLIGFLVKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMIELEARRERVRQNVAQEPISFSFFDLTWSYWKLSVINLVLKKEEMERNSKEGNLRKVINTG
QVAGPNLKSFIRQFPTGSEGAQDVEGLTTRDSLRSSEPVGSGQTFRPGTWQPPT

Lsi05G005970 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome