CuGenDBv2

Gene ID	Lsi06G006720
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Acetohydroxy-acid synthase small subunit
Genome location	chr06:10419824..10426999
RNA-Seq Expression	Lsi06G006720
Synteny	Lsi06G006720
Gene Ontology terms	GO:0009097 - isoleucine biosynthetic process (biological process) GO:0009099 - valine biosynthetic process (biological process) GO:0050790 - regulation of catalytic activity (biological process) GO:0005737 - cytoplasm (cellular component) GO:1990610 - acetolactate synthase regulator activity (molecular function)
InterPro domains	IPR004789 - Acetolactate synthase, small subunit IPR019455 - Acetolactate synthase, small subunit, C-terminal IPR027271 - Acetolactate synthase/Transcription factor NikR, C-terminal

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0038202.1 acetolactate synthase small subunit 1 [Cucumis melo var. makuwa]

4.0e-44

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VEDISR+PQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH
        APFWRFSAASYPDLE+SRP D FVGDAIQS NG +PTST  +           GQ+ D H
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH

KAG6590112.1 Acetolactate synthase small subunit 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]

1.3e-42

71.22

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L  ++ +VEDISR+PQVERELVLMKLNAD  TRAE+   +              + + VTGDPGKL AVERNF KFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST
        APFWRFSAASYPDL++SRP D FVGDA QSLNGNIPTST
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST

XP_008443657.1 PREDICTED: acetolactate synthase small subunit 1, chloroplastic-like [Cucumis melo]

4.0e-44

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VEDISR+PQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH
        APFWRFSAASYPDLE+SRP D FVGDAIQS NG +PTST  +           GQ+ D H
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH

XP_038880022.1 acetolactate synthase small subunit 2, chloroplastic-like isoform X1 [Benincasa hispida]

1.6e-45

68.21

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VEDISR+PQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQMGQMKDGHRF
        APFWRFSAASYPDLE+SRP DPFVGDAIQSLNG +PTST  +  ++   +F
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQMGQMKDGHRF

XP_038880023.1 acetolactate synthase small subunit 2, chloroplastic-like isoform X2 [Benincasa hispida]

3.2e-46

75.18

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCIMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWRFSAASYPDL
        LN L +++ +VEDISR+PQVERELVLMKLNADS TRAE         VTGDPGKL AVERNFSKFGIRELARTGKIALRREKMGETAPFWRFSAASYPDL
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCIMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWRFSAASYPDL

Query:  EESRPFDPFVGDAIQSLNGNIPTSTHQMGQMKDGHRF
        E+SRP DPFVGDAIQSLNG +PTST  +  ++   +F
Subjt:  EESRPFDPFVGDAIQSLNGNIPTSTHQMGQMKDGHRF

TrEMBL top hits

e value

%identity

Alignment

A0A1S3B8L7 Acetohydroxy-acid synthase small subunit

1.9e-44

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VEDISR+PQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH
        APFWRFSAASYPDLE+SRP D FVGDAIQS NG +PTST  +           GQ+ D H
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH

A0A5A7T3Z5 Acetohydroxy-acid synthase small subunit

1.9e-44

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VEDISR+PQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH
        APFWRFSAASYPDLE+SRP D FVGDAIQS NG +PTST  +           GQ+ D H
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTSTHQM-----------GQMKDGH

A0A6J1EAN7 Acetohydroxy-acid synthase small subunit

6.2e-43

70.9

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VEDIS+EPQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGI+ELARTGKIALRREKMGETAPFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST
        FSAASYPDLE+SRP + FVGD+IQS+NGNI TST
Subjt:  FSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST

A0A6J1JLL8 Acetohydroxy-acid synthase small subunit

6.2e-43

71.22

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L  ++ +VEDISR+PQVERELVLMKLNAD  TRAE+   +              + + VTGDPGKL AVERNF KFGIRELARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST
        APFWRFSAASYPDL++SRP D FVGDA QSLNGNIPTST
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST

A0A6J1L3C2 Acetohydroxy-acid synthase small subunit

6.2e-43

70.9

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VEDIS+EPQVERELVLMKLNADS TRAE+   +              + + VTGDPGKL AVERNFSKFGI+ELARTGKIALRREKMGETAPFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST
        FSAASYPDLE+SRP + FVGD+IQS+NGNI TST
Subjt:  FSAASYPDLEESRPFDPFVGDAIQSLNGNIPTST

SwissProt top hits

e value

%identity

Alignment

Q1XDQ7 Acetolactate synthase small subunit

1.0e-10

41.57

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAE--------------VTTCIMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRR
        ++I  V+D++  P VEREL+L+K+  +S+TR E              +   ++ + VTGDPGK+ A+E+  +KFGI E+ARTGKI L R
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAE--------------VTTCIMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRR

Q93YZ7 Acetolactate synthase small subunit 2, chloroplastic

2.2e-29

58.77

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VEDIS EPQVEREL+L+K+NA  ++RAE+   +              + + VTGDPGK+ AVERN  KF IRE+ RTGKIALRREKMG TAPFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLEESRP
        FSAASYPDL+E  P
Subjt:  FSAASYPDLEESRP

Q9FFF4 Acetolactate synthase small subunit 1, chloroplastic

1.4e-28

51.49

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VED+S+EP VEREL+L+KLNAD  TR+E+   +              + + VTGDPGK+ A+  N  KFGI+E+ARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGN
        APFWRFSAASYP L +    +        +L GN
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGN

Q9SMC2 Acetolactate synthase small subunit 1, chloroplastic

6.8e-31

62.73

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VED+S+EPQVEREL+L+K++AD K RAEV   +              + + VTGDPGK+ AV+RN SKFGIRE+ARTGKIALRREKMGE+APFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLE
        FSAASYPDLE
Subjt:  FSAASYPDLE

Q9TLY1 Acetolactate synthase small subunit

1.3e-10

41.57

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTC--------------IMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRR
        ++I +V++I+  P VEREL+L+K+NA + +R E+ +               ++ L +TGDPGK+ A+++   KFGI E+ARTGKIAL R
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTC--------------IMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRR

Arabidopsis top hits

e value

%identity

Alignment

AT2G31810.1 ACT domain-containing small subunit of acetolactate synthase protein

1.6e-30

58.77

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VEDIS EPQVEREL+L+K+NA  ++RAE+   +              + + VTGDPGK+ AVERN  KF IRE+ RTGKIALRREKMG TAPFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLEESRP
        FSAASYPDL+E  P
Subjt:  FSAASYPDLEESRP

AT2G31810.2 ACT domain-containing small subunit of acetolactate synthase protein

1.6e-30

58.77

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VEDIS EPQVEREL+L+K+NA  ++RAE+   +              + + VTGDPGK+ AVERN  KF IRE+ RTGKIALRREKMG TAPFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLEESRP
        FSAASYPDL+E  P
Subjt:  FSAASYPDLEESRP

AT2G31810.3 ACT domain-containing small subunit of acetolactate synthase protein

1.6e-30

58.77

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Query:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR
        +++ +VEDIS EPQVEREL+L+K+NA  ++RAE+   +              + + VTGDPGK+ AVERN  KF IRE+ RTGKIALRREKMG TAPFWR
Subjt:  ISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWR

Query:  FSAASYPDLEESRP
        FSAASYPDL+E  P
Subjt:  FSAASYPDLEESRP

AT5G16290.1 VALINE-TOLERANT 1

1.0e-29

51.49

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VED+S+EP VEREL+L+KLNAD  TR+E+   +              + + VTGDPGK+ A+  N  KFGI+E+ARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGN
        APFWRFSAASYP L +    +        +L GN
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGN

AT5G16290.2 VALINE-TOLERANT 1

1.0e-29

51.49

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Query:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET
        LN L +++ +VED+S+EP VEREL+L+KLNAD  TR+E+   +              + + VTGDPGK+ A+  N  KFGI+E+ARTGKIALRREKMGET
Subjt:  LNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCI--------------MFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGET

Query:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGN
        APFWRFSAASYP L +    +        +L GN
Subjt:  APFWRFSAASYPDLEESRPFDPFVGDAIQSLNGN

Sequences

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CDS sequence

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ATGCTGCTATTTATTTTACCTTTAAACTCTTTAGATATTTCAATTTATCAGGTAGAAGATATCTCAAGAGAGCCCCAAGTAGAGCGTGAGCTGGTGCTTATGAAACTCAA
TGCAGATTCAAAAACTCGTGCTGAGGTGACGACTTGCATTATGTTTTTAATTGTGACTGGAGATCCTGGTAAGCTGTTTGCTGTTGAAAGAAATTTCAGCAAGTTTGGAA
TTAGAGAACTTGCTAGAACTGGAAAGATAGCATTGAGAAGGGAGAAAATGGGGGAGACAGCTCCCTTTTGGAGGTTCTCAGCAGCCTCTTATCCTGATCTCGAAGAATCG
AGGCCATTTGACCCCTTTGTAGGGGATGCAATTCAATCACTCAATGGCAACATCCCTACTTCAACCCATCAGATGGGGCAGATGAAGGATGGTCACAGGTTTGTTGCGAG
GTTGAAGAAAGATGTTATGGGCTTGAAAGATCCGATCCAAGAAGTGATTATCGGGGCCAACCTTTATTCTAGGCCTTTTGAAGAACCGAGTAGTAAAGAACCAGAAATGG
ATGCTTCGAATATCCAGGGTATGGGTAAAGACGCTTCTAGAAAGGAAATGGGTTGGGAAAAGGAGATGAGCCTCGAACCTGAGGATCCAAATGGTGGTAAGATTAGTGTT
TCTTCTCCTTCGTTGGCTCTTAGTCCTCCTTTGGCCGAGAGGGTGTTGGGTGTGGGGGGCTATCTCTCAAAGGCTCGTGGGGTGGGGTCCATTGCCATTGCCTATGGGTC
TTTCGTCTGGGTTGGTGTTGGGGGGAGGGGATTCTGGTCAGTTTCTGTTGTCGAGTTCCTTCCCAAATTTTGGTGGTGCCTTACTATTATGCCAGTTTACTCTTTTGGGG
AGGGGTGTTCTGATTTAGCTTCTTGGTCTAATGCTGTGGGTGTTAGGTTAAGGGGTGGAGGTGTGCTGCTGCCCTATGGGGTTTTGATGCGTGACCAATTCCCTTTCTCT
AATCCTCTCTTGGTTCATTTCCTGATTTCCAATGGGGAGTCTTACGGGTTCAGGCAAGGGGGGTGGGGTGCGCTAGCAACTGTCGGAGTCCAAAGGGTGGAAAATGAAAA
GGTCAAAAGGTGGTAA

mRNA sequence

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Protein sequence

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MLLFILPLNSLDISIYQVEDISREPQVERELVLMKLNADSKTRAEVTTCIMFLIVTGDPGKLFAVERNFSKFGIRELARTGKIALRREKMGETAPFWRFSAASYPDLEES
RPFDPFVGDAIQSLNGNIPTSTHQMGQMKDGHRFVARLKKDVMGLKDPIQEVIIGANLYSRPFEEPSSKEPEMDASNIQGMGKDASRKEMGWEKEMSLEPEDPNGGKISV
SSPSLALSPPLAERVLGVGGYLSKARGVGSIAIAYGSFVWVGVGGRGFWSVSVVEFLPKFWWCLTIMPVYSFGEGCSDLASWSNAVGVRLRGGGVLLPYGVLMRDQFPFS
NPLLVHFLISNGESYGFRQGGWGALATVGVQRVENEKVKRW

Lsi06G006720 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome