CuGenDBv2

Gene ID	Lsi06G007850
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease H
Genome location	chr06:15969647..15970216
RNA-Seq Expression	Lsi06G007850
Synteny	Lsi06G007850
Gene Ontology terms	GO:0006310 - DNA recombination (biological process) GO:0015074 - DNA integration (biological process) GO:0071897 - DNA biosynthetic process (biological process) GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic (biological process) GO:0005634 - nucleus (cellular component) GO:0030430 - host cell cytoplasm (cellular component) GO:0003723 - RNA binding (molecular function) GO:0003887 - DNA-directed DNA polymerase activity (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0033845.1 uncharacterized protein E6C27_scaffold43059G00190 [Cucumis melo var. makuwa]

1.8e-77

77.01

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK+SL GRIAKWQVLL EYDI+YVTRKA+KG AI DCLA  PVEDYEPM  EF DED+M +   LQD E WTM FDGATNEVGHGVGAIL S
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHI
           K Y LT KLYFDCT+NMAEYEACSMGVQMA++MKIKKL+VY D LL +HQL GEWET D KLIPY +YIRELAQNFESITF+H+
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHI

KAA0036934.1 uncharacterized protein E6C27_scaffold86G00070 [Cucumis melo var. makuwa]

2.8e-70

67.89

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTI-KALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK SLSGRIAKWQVLLSEYDIVYVT+KAIKG A+AD LA  PV DYEPM  +FLDE+I  + K  +D ETWTMLFDGA+NE+GHG+G +LIS
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTI-KALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK
         + K++SLTAKL F+CT+N+AEYEAC MG+Q+A++M IKKLKV GD +LV+HQ+  EWE RD KL+PY QY+ +L+QNFE I+F+H+ R+
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

XP_016899404.1 PREDICTED: uncharacterized protein LOC107990505 [Cucumis melo]

2.8e-70

76.74

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK SLSGRIAKWQVL SEYDI+YVTR  +KG AI DCLA+LPVEDYE M FEFLDED+MT+    QD E WTM FDGATNEVGHGVGAIL  
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI
        P  K Y LTAKLYFDCTNNMAEYE CSMGV+MA+++KIKKL+VY D LLV+HQL  EWETRD KLIPY++YI
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI

XP_022147189.1 LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia]

8.2e-70

67.71

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAIL
        MDPIKYIFEK SLSG IA+WQVLLSEYDIVYVT+KAIKG A+AD LA+ P+ DY P+ F+F DE I TI A +   D +TWTM+FDGA+NE+GHG+GAIL
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAIL

Query:  ISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK
        ISPK +LY L A+L FDC +NMAEYEACSMGVQ A +MK+KKLKV+GD +LV+HQL GEWETRD KL+PY Q+I EL+Q F+ I+F+++ R+
Subjt:  ISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

XP_022158986.1 LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]

1.9e-71

69.27

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAIL
        MDPIKYIFEK SLSGRIA+WQVLLSEYDIVYVTRKAIKG A+AD LA+ P+ DY P+ F+F DE I TI A +   D +TWTM+FDGA+NE+GHG+G IL
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAIL

Query:  ISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK
        ISPK +LY LTA+L FDCT+NMAEYEACSMGVQ A +MK+KKLKV+GD +LV+HQL GEWETRD KL+PY Q I EL+Q F+ I+F+++ R+
Subjt:  ISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

TrEMBL top hits

e value

%identity

Alignment

A0A1S4DTT9 uncharacterized protein LOC107990505

1.4e-70

76.74

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK SLSGRIAKWQVL SEYDI+YVTR  +KG AI DCLA+LPVEDYE M FEFLDED+MT+    QD E WTM FDGATNEVGHGVGAIL  
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI
        P  K Y LTAKLYFDCTNNMAEYE CSMGV+MA+++KIKKL+VY D LLV+HQL  EWETRD KLIPY++YI
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI

A0A5A7T0A1 Ribonuclease H

1.4e-70

67.89

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTI-KALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK SLSGRIAKWQVLLSEYDIVYVT+KAIKG A+AD LA  PV DYEPM  +FLDE+I  + K  +D ETWTMLFDGA+NE+GHG+G +LIS
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTI-KALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK
         + K++SLTAKL F+CT+N+AEYEAC MG+Q+A++M IKKLKV GD +LV+HQ+  EWE RD KL+PY QY+ +L+QNFE I+F+H+ R+
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

A0A5A7UKV4 Retrovirus-related Pol polyprotein from transposon 17.6

1.4e-70

76.74

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK SLSGRIAKWQVL SEYDI+YVTR  +KG AI DCLA+LPVEDYE M FEFLDED+MT+    QD E WTM FDGATNEVGHGVGAIL  
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI
        P  K Y LTAKLYFDCTNNMAEYE CSMGV+MA+++KIKKL+VY D LLV+HQL  EWETRD KLIPY++YI
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI

A0A5D3BPI8 RNase H domain-containing protein

8.8e-78

77.01

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS
        MDPIKYIFEK+SL GRIAKWQVLL EYDI+YVTRKA+KG AI DCLA  PVEDYEPM  EF DED+M +   LQD E WTM FDGATNEVGHGVGAIL S
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILIS

Query:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHI
           K Y LT KLYFDCT+NMAEYEACSMGVQMA++MKIKKL+VY D LL +HQL GEWET D KLIPY +YIRELAQNFESITF+H+
Subjt:  PKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHI

A0A6J1E2J7 Ribonuclease H

9.4e-72

69.27

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Query:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAIL
        MDPIKYIFEK SLSGRIA+WQVLLSEYDIVYVTRKAIKG A+AD LA+ P+ DY P+ F+F DE I TI A +   D +TWTM+FDGA+NE+GHG+G IL
Subjt:  MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAIL

Query:  ISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK
        ISPK +LY LTA+L FDCT+NMAEYEACSMGVQ A +MK+KKLKV+GD +LV+HQL GEWETRD KL+PY Q I EL+Q F+ I+F+++ R+
Subjt:  ISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

SwissProt top hits

e value

%identity

Alignment

Q9HSF6 Ribonuclease HI

2.7e-07

32.11

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Query:  FDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE
        FDGA+  N     VG +L+S    + +         TNN AEY+A    ++ A +     +++ GD  LV  QL G W+T DP L       REL   F+
Subjt:  FDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE

Query:  SITFEHIMR
          +  H+ R
Subjt:  SITFEHIMR

Arabidopsis top hits

e value

%identity

Alignment

AT3G01410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.3e-12

29.23

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Query:  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWET
        +   ++T   ++  ++ T+ FDGA+  N    G GA+L +    +     +   + TNN+AEY A  +G++ A +   K + V GD +LV  Q+ G W+T
Subjt:  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWET

Query:  RDPKLIPYHQYIRELAQNFESITFEHIMRK
          PK+    +  +EL  +F++   +HI R+
Subjt:  RDPKLIPYHQYIRELAQNFESITFEHIMRK

AT3G01410.2 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.3e-12

29.23

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Query:  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWET
        +   ++T   ++  ++ T+ FDGA+  N    G GA+L +    +     +   + TNN+AEY A  +G++ A +   K + V GD +LV  Q+ G W+T
Subjt:  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWET

Query:  RDPKLIPYHQYIRELAQNFESITFEHIMRK
          PK+    +  +EL  +F++   +HI R+
Subjt:  RDPKLIPYHQYIRELAQNFESITFEHIMRK

Sequences

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CDS sequence

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ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCAT
AAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGG
ATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAG
TTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCT
TTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTG
AGCATATCATGCGAAAATAA

mRNA sequence

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Protein sequence

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MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAK
LYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

Lsi06G007850 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome