CuGenDBv2

Gene ID	Lsi06G013710
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	protein MIS12 homolog
Genome location	chr06:24275866..24279262
RNA-Seq Expression	Lsi06G013710
Synteny	Lsi06G013710
Gene Ontology terms	GO:0000278 - mitotic cell cycle (biological process) GO:0051276 - chromosome organization (biological process) GO:0000776 - kinetochore (cellular component) GO:0005634 - nucleus (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0058004.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

3.2e-58

60.67

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL + + R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  -------------------GFFNAKLDDIQEFLADLKTL
                           GF NAKLDDIQEFL+DLK +
Subjt:  -------------------GFFNAKLDDIQEFLADLKTL

KAA0058032.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

9.4e-58

60.25

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL + + R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  -------------------GFFNAKLDDIQEFLADLKTL
                           GF NAKLDDIQEFL+DLK +
Subjt:  -------------------GFFNAKLDDIQEFLADLKTL

XP_004138204.3 protein MIS12 homolog isoform X1 [Cucumis sativus]

2.5e-58

60.92

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTE SDRSQDLTL + + R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        DESPGVTSISHD+DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  ------------------GFFNAKLDDIQEFLADLKTL
                          GF NAKLDDIQEFL+DLK +
Subjt:  ------------------GFFNAKLDDIQEFLADLKTL

XP_031742242.1 protein MIS12 homolog isoform X2 [Cucumis sativus]

7.4e-79

71.24

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLVREYRGSEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPG
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTE SDRSQDLTLVREYR SE WSNWV IS WPCG+ST  I VFQ LKDESPG
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLVREYRGSEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPG

Query:  VTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ-----------------------------
        VTSISHD+DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                             
Subjt:  VTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ-----------------------------

Query:  -------------GFFNAKLDDIQEFLADLKTL
                     GF NAKLDDIQEFL+DLK +
Subjt:  -------------GFFNAKLDDIQEFLADLKTL

XP_038879945.1 protein MIS12 homolog [Benincasa hispida]

1.1e-58

61.92

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAF FYQSQASA LKTEGSDRSQDLTL +   R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        D+SPGVTSISHDYDV LDTELDFLRNKLSE RKENIVL+QELQALERQTA+SNSQINH NEALQLYEQNSVNEMFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  -------------------GFFNAKLDDIQEFLADLKTL
                           G  NAKLDDI+ FL DLKTL
Subjt:  -------------------GFFNAKLDDIQEFLADLKTL

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LPL2 Uncharacterized protein

7.8e-58

60.5

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTE SDRSQDLTL + + R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        DESPGVTSISHD+DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  ------------------GFFNAKLDDIQEFLADLKTL
                          GF NAKLDDIQEFL+ LK +
Subjt:  ------------------GFFNAKLDDIQEFLADLKTL

A0A5A7UQ95 Centromere protein Mis12

4.6e-58

60.25

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL + + R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  -------------------GFFNAKLDDIQEFLADLKTL
                           GF NAKLDDIQEFL+DLK +
Subjt:  -------------------GFFNAKLDDIQEFLADLKTL

A0A5A7US57 Centromere protein Mis12

1.6e-58

60.67

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL + + R          ++ W   C     S+     L   
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL-VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--K

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ                        
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ------------------------

Query:  -------------------GFFNAKLDDIQEFLADLKTL
                           GF NAKLDDIQEFL+DLK +
Subjt:  -------------------GFFNAKLDDIQEFLADLKTL

A0A6J1JD43 protein MIS12 homolog isoform X1

1.9e-56

59.84

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL----VREYRGS------EPWSNWV--WISGWPCGRSTVSI
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L    VR +  S        W  +        P G S  S 
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL----VREYRGS------EPWSNWV--WISGWPCGRSTVSI

Query:  IVFQFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---------------
               DESP VTS+S D  YDVDLDTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N  NEALQLYEQNSVNEMFQ               
Subjt:  IVFQFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---------------

Query:  -----------------------------GFFNAKLDDIQEFLADLKTL
                                     GF NAKLDDIQEFLADLKTL
Subjt:  -----------------------------GFFNAKLDDIQEFLADLKTL

A0A6J1JME3 protein MIS12 homolog isoform X2

1.5e-56

60.08

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL----VREYRGS------EPWSNWV--WISGWPCGRSTVSI
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L    VR +  S        W  +        P G S  S 
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL----VREYRGS------EPWSNWV--WISGWPCGRSTVSI

Query:  IVFQFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---------------
               DESP VTS+S D  YDVDLDTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N  NEALQLYEQNSVNEMFQ               
Subjt:  IVFQFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---------------

Query:  ----------------------------GFFNAKLDDIQEFLADLKTL
                                    GF NAKLDDIQEFLADLKTL
Subjt:  ----------------------------GFFNAKLDDIQEFLADLKTL

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

1.8e-27

42.86

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL---VREYRG------SEPWSNWVWISGWPCGRSTVSIIVF
        MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD+ +   L   +   RG            W   S   C       +  
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL---VREYRG------SEPWSNWVWISGWPCGRSTVSIIVF

Query:  QFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ
         F+  +S   +S+  D  YD++LD ELD LR+KL+   K ++ L  ELQALER + S    +  +NEAL+LY+++S++E+F+
Subjt:  QFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

1.3e-28

42.86

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL---VREYRG------SEPWSNWVWISGWPCGRSTVSIIVF
        MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD+ +   L   +   RG            W   S   C       +  
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL---VREYRG------SEPWSNWVWISGWPCGRSTVSIIVF

Query:  QFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ
         F+  +S   +S+  D  YD++LD ELD LR+KL+   K ++ L  ELQALER + S    +  +NEAL+LY+++S++E+F+
Subjt:  QFLKDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ

Sequences

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CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGGTTGATGACGCTTTTGA
TTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGATAGATCCCAGGATCTTACCCTGGTAAGGGAATATCGCGGGTCCGAACCTTGGTCCAATTGGG
TCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCATTGTTTTTCAGTTCCTGAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTG
GATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAACTACAAGCTTTGGAAAGGCAAACTGCTTCCAG
TAACTCCCAAATCAATCATTTGAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGGTTTCTTTAATGCTAAGCTGGATGATATTCAAGAAT
TTTTAGCTGATTTGAAGACCCTTTGA

mRNA sequence

Show/hide mRNA sequence

TGTAAGCCCGCTCCGCGTCCCCATCATTCGCCACAGCCATTTTGGCGGCAAAATTTCTCCATTTATGCCAAAGCTTTGGAGCTTCCATTTATCTCTCCATTTGAATTTCT
CGTCGTCTTCTTGATTGGGAAAGAAGCTTTTTCAGGAAGCGAAAGGGAAATTTTGATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCACAGC
TCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGGTTGATGACGCTTTTGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGATAGATCC
CAGGATCTTACCCTGGTAAGGGAATATCGCGGGTCCGAACCTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCATTGTTTTTCA
GTTCCTGAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAG
AGAATATTGTATTGAGCCAAGAACTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAATCATTTGAACGAAGCATTACAATTATATGAGCAAAATTCT
GTGAATGAGATGTTCCAGGGTTTCTTTAATGCTAAGCTGGATGATATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGAAATCATTTCACTATGCCATCAACCTTACT
GTATATGATGCTTGGTGTGAAACTTGTCACTATGAGTTCTTCAATGTCTTAACTTGATTTAGCTGTTGTGAGATATTTTAAACCAAATTTGTATAATATATCATTTGGAT
TGATGACCATCCTTAGCAATCTTCTTAGAGAGTTTGCAGATGGGCCTTCAGTAGGTGTTGGCTCAATCCTATAAATTATGATGGTTCACATTAGACTTTTGAAGCTTCTC
TCATTACCTTTTCG

Protein sequence

Show/hide protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLVREYRGSEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPGVTSISHDYDV
DLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQGFFNAKLDDIQEFLADLKTL

Lsi06G013710 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome