CuGenDBv2

Gene ID	Lsi08G012240
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerase subunit beta''
Genome location	chr08:20718492..20721078
RNA-Seq Expression	Lsi08G012240
Synteny	Lsi08G012240
Gene Ontology terms	GO:0006351 - transcription, DNA-templated (biological process) GO:0005840 - ribosome (cellular component) GO:0009507 - chloroplast (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR042102 - RNA polymerase Rpb1, domain 3 superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

QJS52311.1 RNA polymerase beta'' subunit [Indofevillea khasiana]

1.1e-34

76.64

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

QWL21619.1 RNA polymerase beta'' subunit [Caryodaphnopsis malipoensis]

1.2e-33

75.73

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Query:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI
        MEVLMAEWVDL FHNKVIDG A+KRLISRLIDHFGMAYTSH LDQ+KTLGFQQATATSISLGIDDLLT+PSKGWLV     + LI     H+G  YT   
Subjt:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI

Query:  LDQ
        L Q
Subjt:  LDQ

QWQ50133.1 RNA polymerase beta'' subunit [Cyclanthera pedata]

3.1e-34

75.7

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     +  I     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

YP_009525176.1 RNA polymerase beta subunit [Hodgsonia macrocarpa]

1.1e-34

76.64

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

YP_010131084.1 RNA polymerase beta'' subunit [Benincasa hispida]

6.3e-35

77.57

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

TrEMBL top hits

e value

%identity

Alignment

A0A346QNZ0 DNA-directed RNA polymerase subunit beta''

5.2e-35

76.64

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

A0A6G6A8I5 DNA-directed RNA polymerase subunit beta''

5.8e-34

74.77

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  +LVFHNKVIDGTA+KRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

A0A6M4RBT8 DNA-directed RNA polymerase subunit beta''

9.8e-34

73.83

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T +NMEVLMAE  +LVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + L+     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

A0A6M4SPN7 DNA-directed RNA polymerase subunit beta''

5.2e-35

76.64

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

A0A7T3QNJ7 RNA polymerase beta'' subunit

3.1e-35

77.57

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Query:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY
        T RNMEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +
Subjt:  TPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAY

Query:  TSHILDQ
            L Q
Subjt:  TSHILDQ

SwissProt top hits

e value

%identity

Alignment

Q06H07 DNA-directed RNA polymerase subunit beta''

6.6e-35

74.76

Show/hide

Query:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI
        MEVLMAE  DLVFHNKVIDGTA+KRLISRLIDHFGMAYTSHILDQ+KT+GFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +    
Subjt:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI

Query:  LDQ
        L Q
Subjt:  LDQ

Q09X27 DNA-directed RNA polymerase subunit beta''

8.6e-35

75.73

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Query:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI
        MEVLMAE  DLVF NKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGF+QATATSISLGIDDLLTIPSKGWLV     +RLI     H+G  +    
Subjt:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI

Query:  LDQ
        L Q
Subjt:  LDQ

Q49L08 DNA-directed RNA polymerase subunit beta''

2.3e-35

76.7

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Query:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI
        MEVLMAE V+LVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +    
Subjt:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI

Query:  LDQ
        L Q
Subjt:  LDQ

Q4VZP3 DNA-directed RNA polymerase subunit beta''

7.7e-36

77.67

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Query:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI
        MEVLMAE  DLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +    
Subjt:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI

Query:  LDQ
        L Q
Subjt:  LDQ

Q9THV5 DNA-directed RNA polymerase subunit beta''

6.6e-35

75.73

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Query:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI
        MEVLMAE  +LVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     + LI     H+G  +    
Subjt:  MEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHI

Query:  LDQ
        L Q
Subjt:  LDQ

Arabidopsis top hits

e value

%identity

Alignment

ATCG00170.1 DNA-directed RNA polymerase family protein

2.2e-33

73.74

Show/hide

Query:  MAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHILDQ
        MAE  +LVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQ+KTLGFQQATATSISLGIDDLLTIPSKGWLV     +  I     H+G  +    L Q
Subjt:  MAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHILDQ

Sequences

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CDS sequence

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ATGACACCGCGTAATATGGAGGTACTTATGGCGGAATGGGTCGATCTGGTCTTTCACAATAAAGTGATAGATGGAACTGCCATTAAACGACTTATTAGCAGATTAATAGA
TCACTTCGGAATGGCATATACATCACACATCCTGGATCAACTAAAGACTCTGGGGTTCCAGCAGGCCACTGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAA
TCCCTTCTAAGGGATGGCTAGTCNATGGAACTGCCATTAAACGACTTATTAGCAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTGGATCAACTAAAG
ACTCTGGGGTTCCAACAGGCCACTGCTACATCCATTTCAGACAAACCCGTTACACTAAGACCCATGAAACACGTAAACTTGGTTGTTTCGTGA

mRNA sequence

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Protein sequence

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MTPRNMEVLMAEWVDLVFHNKVIDGTAIKRLISRLIDHFGMAYTSHILDQLKTLGFQQATATSISLGIDDLLTIPSKGWLVXGTAIKRLISRLIDHFGMAYTSHILDQLK
TLGFQQATATSISDKPVTLRPMKHVNLVVS

Lsi08G012240 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome