CuGenDBv2

Gene ID	Lsi08G016700
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Genome location	chr08:24455263..24457481
RNA-Seq Expression	Lsi08G016700
Synteny	Lsi08G016700
Gene Ontology terms	GO:0006360 - transcription by RNA polymerase I (biological process) GO:0006366 - transcription by RNA polymerase II (biological process) GO:0042797 - tRNA transcription by RNA polymerase III (biological process) GO:0005665 - RNA polymerase II, core complex (cellular component) GO:0005666 - RNA polymerase III complex (cellular component) GO:0005736 - RNA polymerase I complex (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000268 - DNA-directed RNA polymerase, subunit N/Rpb10 IPR020789 - RNA polymerases, subunit N, zinc binding site IPR023580 - RNA polymerase subunit RPB10

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0057130.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucumis melo var. makuwa]

1.2e-29

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

KAB5552457.1 hypothetical protein DKX38_009768 [Salix brachista]

3.3e-30

78.16

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETFSARLEWSYVLV
        MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNR   G  L   T + R + S  +V
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETFSARLEWSYVLV

KAG6598222.1 DNA-directed RNA polymerase subunit 10-like protein, partial [Cucurbita argyrosperma subsp. sororia]

1.2e-29

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

XP_004138685.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucumis sativus]

1.2e-29

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

XP_022961651.1 DNA-directed RNA polymerase subunit 10-like protein [Cucurbita moschata]

1.2e-29

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

TrEMBL top hits

e value

%identity

Alignment

A0A0R0L5R6 Uncharacterized protein

2.7e-30

85.53

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETF
        MIIPVRCFTCGKVIGNKWD YLDLLQ+DYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN  S  +Y T   F
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETF

A0A5A7UPU3 DNA-directed RNA polymerases I, II, and III subunit RPABC5

6.0e-30

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

A0A5N5MBL4 Uncharacterized protein

1.6e-30

78.16

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETFSARLEWSYVLV
        MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNR   G  L   T + R + S  +V
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETFSARLEWSYVLV

A0A6J1KEH3 DNA-directed RNA polymerase subunit 10-like protein

6.0e-30

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

A0A6J1KRF4 DNA-directed RNA polymerases I, II, and III subunit RPABC5

6.0e-30

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

SwissProt top hits

e value

%identity

Alignment

Q32P78 DNA-directed RNA polymerases I, II, and III subunit RPABC5

1.0e-26

83.87

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNY
        MIIPVRCFTCGK++GNKW+ YL LLQA+Y+EGDALDALGL RYCCRRML+ HVDLIEKLLNY
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNY

Q39290 DNA-directed RNA polymerases I, II, and III subunit RPABC5

9.9e-30

93.65

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

Q55AB6 DNA-directed RNA polymerases I, II, and III subunit rpabc5

6.0e-27

88.71

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNY
        MIIPVRCFTCGKVIGNKWD YL LLQ DY+EGDALDAL L RYCCRRML+THVDLIEKLLNY
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNY

Q8LFJ6 DNA-directed RNA polymerases II, IV and V subunit 10

9.9e-30

93.65

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

Q9SYA6 DNA-directed RNA polymerase subunit 10-like protein

2.0e-30

93.65

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MI+PVRCFTCGKVIGNKWD YL+LLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

Arabidopsis top hits

e value

%identity

Alignment

AT1G11475.1 RNA polymerases N / 8 kDa subunit

7.0e-31

93.65

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

AT1G61700.1 RNA polymerases N / 8 kDa subunit

1.4e-31

93.65

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
        MI+PVRCFTCGKVIGNKWD YL+LLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYN

Sequences

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CDS sequence

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ATGATCATCCCAGTGCGTTGCTTCACTTGCGGAAAGGTGATTGGAAACAAGTGGGATCATTACCTTGATCTTTTACAGGCAGACTATTCAGAAGGGGATGCACTTGATGC
ACTGGGCTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGACTCATGTTGACCTTATTGAGAAGCTTCTTAATTACAATAGAGTCAGCATTGGCGTTTACTTAACTATGG
AAACATTCAGTGCCCGACTCGAGTGGAGCTATGTCTTGGTGTCCCGAGTTGGGAACAAGAAGAAGGGAAGGTGCCGGTCGCCGGATGTGGCATCTTCTCGATCTCATGAA
TGGAGAGTCTACTTTGAAATCGCTTGCCTTGCACCTACCGTTGAGTACTTTGTCTGA

mRNA sequence

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AAAAAAGATTTAAAAAAGAAATACCGAAGGTGAAATTGCGTGGGTTGTGCAGAAAGAAAGCGAAGAAGATGATCATCCCAGTGCGTTGCTTCACTTGCGGAAAGGTGATT
GGAAACAAGTGGGATCATTACCTTGATCTTTTACAGGCAGACTATTCAGAAGGGGATGCACTTGATGCACTGGGCTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGAC
TCATGTTGACCTTATTGAGAAGCTTCTTAATTACAATAGAGTCAGCATTGGCGTTTACTTAACTATGGAAACATTCAGTGCCCGACTCGAGTGGAGCTATGTCTTGGTGT
CCCGAGTTGGGAACAAGAAGAAGGGAAGGTGCCGGTCGCCGGATGTGGCATCTTCTCGATCTCATGAATGGAGAGTCTACTTTGAAATCGCTTGCCTTGCACCTACCGTT
GAGTACTTTGTCTGA

Protein sequence

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MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNRVSIGVYLTMETFSARLEWSYVLVSRVGNKKKGRCRSPDVASSRSHE
WRVYFEIACLAPTVEYFV

Lsi08G016700 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome