CuGenDBv2

Gene ID	Lsi10G003520
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Mediator of RNA polymerase II transcription subunit 32
Genome location	chr10:5509062..5510102
RNA-Seq Expression	Lsi10G003520
Synteny	Lsi10G003520
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0009631 - cold acclimation (biological process) GO:0010150 - leaf senescence (biological process) GO:0048364 - root development (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR033244 - Mediator of RNA polymerase II transcription subunit 32

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0045925.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

1.9e-69

95.97

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Query:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI
        VSDMDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ TTGLPPI
Subjt:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI

Query:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+ED TQ
Subjt:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

TYK13665.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

5.0e-70

96.64

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Query:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI
        VSDMDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ TTGLPPI
Subjt:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI

Query:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+EDTTQ
Subjt:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

XP_004148855.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus]

1.0e-67

95.89

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Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ATT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+ED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

XP_008457936.1 PREDICTED: mediator of RNA polymerase II transcription subunit 32 [Cucumis melo]

1.6e-68

96.58

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Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ TTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+EDTTQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

XP_038902813.1 mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida]

1.6e-68

96.58

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Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQAATTG+PPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+ED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LIN7 Uncharacterized protein

5.0e-68

95.89

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Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ATT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+ED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

A0A1S3C6T6 mediator of RNA polymerase II transcription subunit 32

7.8e-69

96.58

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Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ TTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+EDTTQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

A0A5A7TWZ7 Mediator of RNA polymerase II transcription subunit 32

9.2e-70

95.97

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Query:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI
        VSDMDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ TTGLPPI
Subjt:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI

Query:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+ED TQ
Subjt:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

A0A5D3CQ46 Mediator of RNA polymerase II transcription subunit 32

2.4e-70

96.64

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Query:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI
        VSDMDSLVDSLNNAYQEFVAGAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQ+ TTGLPPI
Subjt:  VSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPI

Query:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARF+EDTTQ
Subjt:  SAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

A0A6J1JEJ1 mediator of RNA polymerase II transcription subunit 32-like isoform X1

1.2e-66

92.21

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Query:  MKVSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAA--TTG
        + VSDMDSLVDSLNNAYQEFV+GAANVLE NEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGK+GQAA  TTG
Subjt:  MKVSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAA--TTG

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSG-AAASASAHSHQSAPFDARFSEDTTQ
        LPPISA+RLEQMSKAVRWLVIELQHGSG AAASASAHSH SAPFDARF+ED TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSG-AAASASAHSHQSAPFDARFSEDTTQ

SwissProt top hits

e value

%identity

Alignment

Q84VW5 Mediator of RNA polymerase II transcription subunit 32

1.2e-45

69.28

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Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKAGQAATTG----
        MD++VDSLN AY++FV  +A VLE  E++G QK   TD ALENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG   + AG    AA TG    
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKAGQAATTG----

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        LPPISAVRLEQMS+AVRWLV+ELQ GSG  A  S HS  S  FD+RFSED+TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

Arabidopsis top hits

e value

%identity

Alignment

AT1G11760.1 MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

8.3e-47

69.28

Show/hide

Query:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKAGQAATTG----
        MD++VDSLN AY++FV  +A VLE  E++G QK   TD ALENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG   + AG    AA TG    
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKAGQAATTG----

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ
        LPPISAVRLEQMS+AVRWLV+ELQ GSG  A  S HS  S  FD+RFSED+TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

Sequences

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CDS sequence

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mRNA sequence

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ATGAAAGTATCTGATATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAATGTCCTTGAAGTAAATGAAGCCTCTGGG
GCTCAGAAGACAGCAGCAACAGATGCTGCTCTTGAGAATTTTAAACAGAAATGGGAACTATTCAGGGTTGCTTGTGATCAAGCTGAGGAGTTTGTGGAGTCTGTG
AAGCAAAGGATAGGATCAGAGTGCCTTGTTGATGAGGCCACTGGTTCACTGGCTGGCAAGGCTGGGCAGGCCGCCACCACTGGCCTTCCTCCGATCAGTGCTGTT
CGGTTGGAACAGATGAGCAAAGCTGTAAGATGGCTTGTGATTGAGTTGCAGCATGGCTCAGGAGCTGCTGCTTCTGCTTCAGCGCACTCTCATCAGTCTGCTCCT
TTTGATGCTAGATTTAGTGAGGATACAACCCAATAGGTACCTATCTTATGTTCTTTGTACTTGTTTGTTTGCTTTTCTTGGAGTATGGGTGGGGAGAAATTGTCT
CTAGTATGTATATGTATTTTTTGTGTGAACGTGGGACTCGAAACTCTAACTTCTTAATCGAGAATATATATTTTAACCAATTGACCTATGCTTAAGTTATCGAGA
TTGGATTCATTATGCCATATGTTTTCGTCTTGATAGTTGGATTGGTCTAATTAAGTATTAATATTTGGGTCCAAGTCCAATTGAGCCCAAAAATAGGCTTAATTA
GGCTAAAGGCTAGGTCCATTGACCAACTAAGCCCAATTCCACTTAAGGCCCATGGAAACTCCATAAATAGGAGTCTTACCTTTTTTTTTTTTTTTGGAGATGGAA
AGAATTGAAGTTATGTAGAATTGAAGATTGAAGCTTTCAAGATTTGAAGATCAAAGTTCCCAAGATTTAAAGACCGAAG

Protein sequence

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MKVSDMDSLVDSLNNAYQEFVAGAANVLEVNEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKAGQAATTGLPPISAV
RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFSEDTTQ

Lsi10G003520 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome