CuGenDBv2

Gene ID	Lsi10G009190
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	Lysine--tRNA ligase
Genome location	chr10:13665338..13671226
RNA-Seq Expression	Lsi10G009190
Synteny	Lsi10G009190
Gene Ontology terms	GO:0006430 - lysyl-tRNA aminoacylation (biological process) GO:0048481 - plant ovule development (biological process) GO:0005739 - mitochondrion (cellular component) GO:0005829 - cytosol (cellular component) GO:0009507 - chloroplast (cellular component) GO:0000049 - tRNA binding (molecular function) GO:0003677 - DNA binding (molecular function) GO:0003729 - mRNA binding (molecular function) GO:0004824 - lysine-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

TYK21738.1 lysine--tRNA ligase [Cucumis melo var. makuwa]

2.6e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

XP_004149642.1 lysine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus]

2.0e-22

94.74

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

XP_008449893.1 PREDICTED: lysine--tRNA ligase, chloroplastic/mitochondrial isoform X3 [Cucumis melo]

2.6e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

XP_022995985.1 lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima]

2.6e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

XP_038901525.1 lysine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]

2.0e-22

94.74

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L1N3 Lysine--tRNA ligase

9.6e-23

94.74

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

A0A1S3BP13 Lysyl-tRNA synthetase

1.2e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

A0A5A7TFD0 Lysine--tRNA ligase

1.2e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

A0A5D3DE11 Lysine--tRNA ligase

1.2e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

A0A6J1K5F6 Lysine--tRNA ligase

1.2e-22

92.98

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY+DMIANPEVA+TFRKRAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

SwissProt top hits

e value

%identity

Alignment

B2J384 Lysine--tRNA ligase

6.8e-18

73.21

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV
        ELSV V  + ILTKSLLPLPDK+HGLTDV KRYRQRY+D+I NPEV +TFR+RA++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV

Q31KH4 Lysine--tRNA ligase

1.1e-18

73.21

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV
        ELS+ V+ + ILTKSL PLPDK+HGLTDV+KRYRQRY+D+I NPEV ETFR+RAK+
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV

Q5N1D9 Lysine--tRNA ligase

1.1e-18

73.21

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV
        ELS+ V+ + ILTKSL PLPDK+HGLTDV+KRYRQRY+D+I NPEV ETFR+RAK+
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV

Q8DMA9 Lysine--tRNA ligase

3.1e-18

73.21

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV
        ELSV V S+ +LTKSLLPLPDK+HGLTDV+KRYRQRY+D+I NP+V +TFRKRA +
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKV

Q9LJE2 Lysine--tRNA ligase, chloroplastic/mitochondrial

1.9e-23

84.21

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELS+CVNSF ILTKSLLPLPDKYHGLTD+DKRYRQRY+DMIANPEVA+ FR+RAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

Arabidopsis top hits

e value

%identity

Alignment

AT3G11710.1 lysyl-tRNA synthetase 1

3.4e-04

56.25

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Query:  LTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        L D + RYRQR++DMI N EV + FR RAK++
Subjt:  LTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

AT3G13490.1 Lysyl-tRNA synthetase, class II

1.3e-24

84.21

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Query:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM
        ELS+CVNSF ILTKSLLPLPDKYHGLTD+DKRYRQRY+DMIANPEVA+ FR+RAK++
Subjt:  ELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

Sequences

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CDS sequence

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ATGCGGGAGCTTTCTGTTTGTGTGAATTCTTTTGAAATCCTGACAAAGTCATTACTTCCACTTCCTGACAAATATCATGGTTTAACTGATGTTGATAAGCGCTACCGCCA
AAGGTACATAGATATGATTGCAAATCCTGAAGTAGCCGAAACATTCCGAAAAAGAGCAAAGGTGATGTAA

mRNA sequence

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ATGCGGGAGCTTTCTGTTTGTGTGAATTCTTTTGAAATCCTGACAAAGTCATTACTTCCACTTCCTGACAAATATCATGGTTTAACTGATGTTGATAAGCGCTACCGCCA
AAGGTACATAGATATGATTGCAAATCCTGAAGTAGCCGAAACATTCCGAAAAAGAGCAAAGGTGATGTAA

Protein sequence

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MRELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYIDMIANPEVAETFRKRAKVM

Lsi10G009190 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome