CuGenDBv2

Gene ID	LsiUNG000570
Organism	Lagenaria siceraria USVL1VR-Ls (Bottle gourd (USVL1VR-Ls) v1)
Description	bifunctional nitrilase/nitrile hydratase NIT4A
Genome location	chr00:998943..999814
RNA-Seq Expression	LsiUNG000570
Synteny	LsiUNG000570
Gene Ontology terms	GO:0019500 - cyanide catabolic process (biological process) GO:0051410 - detoxification of nitrogen compound (biological process) GO:0018822 - nitrile hydratase activity (molecular function) GO:0047427 - cyanoalanine nitrilase activity (molecular function) GO:0047558 - 3-cyanoalanine hydratase activity (molecular function)
InterPro domains	IPR044149 - Nitrilase/Cyanide hydratase

Homology

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GenBank top hits

e value

%identity

Alignment

XP_004139500.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus]

4.7e-19

98.04

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK EDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

XP_008463648.1 PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo]

3.0e-18

96.08

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTKAEDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

XP_023520715.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]

4.0e-18

94.12

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS STK EDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

XP_038894429.1 bifunctional nitrilase/nitrile hydratase NIT4A isoform X1 [Benincasa hispida]

2.3e-18

96.08

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEI RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTSTSTKAEDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

XP_038894430.1 bifunctional nitrilase/nitrile hydratase NIT4A isoform X2 [Benincasa hispida]

2.3e-18

96.08

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEI RAKFDFDVVGHYARPEVLSLVVRDHP TPVTFTSTSTKAEDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LVI0 CN hydrolase domain-containing protein

2.3e-19

98.04

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK EDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

A0A1S3CLB4 bifunctional nitrilase/nitrile hydratase NIT4A

1.5e-18

96.08

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTKAEDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

A0A5A7SY83 Bifunctional nitrilase/nitrile hydratase NIT4A

1.5e-18

96.08

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTKAEDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

A0A6J1CM33 bifunctional nitrilase/nitrile hydratase NIT4A

2.1e-17

90.2

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLGEI RAKFDFDVVGHYARPEVLSL V+DHPTTPVTFTSTS K EDSCKK
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

A0A6J1I7W0 bifunctional nitrilase/nitrile hydratase NIT4A

3.6e-17

90.2

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS S K EDSC K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

SwissProt top hits

e value

%identity

Alignment

Q3LRV4 Bifunctional nitrilase/nitrile hydratase NIT4B

2.0e-17

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK
        DLGEIARAKFDFDVVGHY+R EVLSL+V+DHPT PVTFTSTSTK ED  K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK

Q42965 Bifunctional nitrilase/nitrile hydratase NIT4A

7.7e-17

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK
        DLGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+KAE S K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK

Q42966 Bifunctional nitrilase/nitrile hydratase NIT4B

7.7e-17

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK
        DLGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+KAE S K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK

Q5QGZ8 Bifunctional nitrilase/nitrile hydratase NIT4A

7.0e-18

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK
        DLGEIARAKFDFDVVGHY+RPEVLSLVV+DHPT PVTFTS STK ED  K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCK

Q6H849 Bifunctional nitrilase/nitrile hydratase NIT4

1.5e-12

79.55

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK
        DLGEI RAKFDFDVVGHYARPEVLSLVV D P  PV+FTS + K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK

Arabidopsis top hits

e value

%identity

Alignment

AT3G44300.1 nitrilase 2

1.7e-11

60.78

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLG++ARAK  FD VGHY+RP+VL L V +HP  PVTF S   KAED   K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

AT3G44310.1 nitrilase 1

1.9e-10

58.82

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLG+IARAK  FD VGHY+RP+VL L V +HP   VTF +   KAED   K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

AT3G44310.2 nitrilase 1

1.9e-10

58.82

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLG+IARAK  FD VGHY+RP+VL L V +HP   VTF +   KAED   K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

AT3G44320.1 nitrilase 3

2.9e-11

56.86

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK
        DLG+IARAK  FDVVGHY++P++ +L V +HP  PVTF +   KAED   K
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

AT5G22300.1 nitrilase 4

1.7e-11

60.42

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Query:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDS
        DLG+IARAKFDFDVVGHY+RPEV SL +R+HP   V+F ++    ++S
Subjt:  DLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDS

Sequences

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CDS sequence

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mRNA sequence

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ATGAATTTTCAGTTTCCACATGGTCTGTTTGTGAATGTTGAAATTCGTAGTTTGAAATTGTGGCAAGCCATTGATTTTTGGCACGATCTTGGAGAAATAGCACGGGCAAA
ATTCGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTACATCTACAAAAGCCG
AAGATTCTTGTAAAAAGTAATATGTACTATTCTCCACACTTCTATCTAGTTCCATCAATTGTTCTAGCCAACGTGTGTTGTTGCCCATGTTTTACTGTTTCCTCTGCGTT
CAGTGTTGTATTATATTCATACTAATTATTCAGAATTGTGAATATTTCTGTTGAAAAGTAAACCTGTTTTAATTCTCTAGTTAAGTTATTAGATGAATTAAAGAATGAGA
ATTGAGTTAATTTAGTAGAGTTTTATGAATTAAAAATTGGAGGTTAATATGGGTTTCATGTATTTAAGTATGAGAGTTATGGTTCATGAGAATCCTATAAATAGGATTGT
TTTGTATTGTTTATTGCAACTAAGTGTGTAGTGAAGAATACACAAAAAGAATTCAATACAAAGTGTGAATCAAGTTTGAAGAGTT

Protein sequence

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MNFQFPHGLFVNVEIRSLKLWQAIDFWHDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKAEDSCKK

LsiUNG000570 (gene) of Bottle gourd (USVL1VR-Ls) v1 genome