CuGenDBv2

Gene ID	MC03g1133
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA-directed RNA polymerases II, IV and V subunit 12
Genome location	MC03:17492922..17493271
RNA-Seq Expression	MC03g1133
Synteny	MC03g1133
Gene Ontology terms	GO:0006351 - transcription, DNA-templated (biological process) GO:0005665 - RNA polymerase II, core complex (cellular component) GO:0005666 - RNA polymerase III complex (cellular component) GO:0005736 - RNA polymerase I complex (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR006591 - RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 IPR029040 - RNA polymerase subunit RPABC4/transcription elongation factor Spt4 IPR039747 - DNA-directed RNA polymerases I, II, and III subunit RPABC4

Homology

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GenBank top hits

e value

%identity

Alignment

EEF34914.1 DNA-directed RNA polymerases I, II, and III 7.0 kDa polypeptide, putative [Ricinus communis]

1.14e-26

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

KAA0031913.1 putative DNA-directed RNA polymerases I, II, and III 7.0 kDa polypeptide [Cucumis melo var. makuwa]

1.32e-26

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

KAG7012179.1 DNA-directed RNA polymerases II, IV and V subunit 12, partial [Cucurbita argyrosperma subsp. argyrosperma]

3.05e-26

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

TXG56999.1 hypothetical protein EZV62_018312 [Acer yangbiense]

6.62e-26

97.73

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLK GDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

XP_015579873.1 DNA-directed RNA polymerases II, IV and V subunit 12 [Ricinus communis]

1.14e-26

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LQM4 Uncharacterized protein

5.52e-27

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

A0A1S4E4H5 DNA-directed RNA polymerases II, IV and V subunit 12

5.52e-27

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

A0A6J1GTY0 DNA-directed RNA polymerases II, IV and V subunit 12

5.52e-27

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

A0A6J1KJQ4 DNA-directed RNA polymerases II, IV and V subunit 12

5.52e-27

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

B9SNE7 DNA-directed RNA polymerases I, II, and III 7.0 kDa polypeptide, putative

5.52e-27

100

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

SwissProt top hits

e value

%identity

Alignment

P53803 DNA-directed RNA polymerases I, II, and III subunit RPABC4

1.3e-10

61.9

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Query:  PQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        P+ +P+ YICG+C  EN +K  D I+CRECGYRI+YKKRT+R
Subjt:  PQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

Q3ZBC0 DNA-directed RNA polymerases I, II, and III subunit RPABC4

1.3e-10

61.9

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Query:  PQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        P+ +P+ YICG+C  EN +K  D I+CRECGYRI+YKKRT+R
Subjt:  PQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

Q63871 DNA-directed RNA polymerases I, II, and III subunit RPABC4

1.3e-10

61.9

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Query:  PQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        P+ +P+ YICG+C  EN +K  D I+CRECGYRI+YKKRT+R
Subjt:  PQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

Q9C8M4 DNA-directed RNA polymerase subunit 12-like protein

1.2e-11

73.17

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Query:  DPQPEP-VSYICGDCGMENTLKQGDVIQCRECGYRILYKKR
        D QPE  V Y+CGDCG EN LK+GDV QCR+CG+RILYKKR
Subjt:  DPQPEP-VSYICGDCGMENTLKQGDVIQCRECGYRILYKKR

Q9FLM8 DNA-directed RNA polymerases II, IV and V subunit 12

1.3e-18

88.64

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDP PEPV+Y+CGDCG ENTLK GDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

Arabidopsis top hits

e value

%identity

Alignment

AT1G53690.1 DNA directed RNA polymerase, 7 kDa subunit

8.6e-13

73.17

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Query:  DPQPEP-VSYICGDCGMENTLKQGDVIQCRECGYRILYKKR
        D QPE  V Y+CGDCG EN LK+GDV QCR+CG+RILYKKR
Subjt:  DPQPEP-VSYICGDCGMENTLKQGDVIQCRECGYRILYKKR

AT5G41010.1 DNA directed RNA polymerase, 7 kDa subunit

9.5e-20

88.64

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Query:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR
        MDP PEPV+Y+CGDCG ENTLK GDVIQCRECGYRILYKKRTRR
Subjt:  MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

Sequences

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CDS sequence

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ATGGATCCTCAACCTGAACCAGTCAGCTACATCTGTGGAGATTGTGGAATGGAGAATACCCTGAAGCAGGGTGATGTTATACAATGCCGAGAGTGTGGTTATCGTATTCT
CTACAAGAAGCGCACCCGTCGC

mRNA sequence

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ATGGATCCTCAACCTGAACCAGTCAGCTACATCTGTGGAGATTGTGGAATGGAGAATACCCTGAAGCAGGGTGATGTTATACAATGCCGAGAGTGTGGTTATCGTATTCT
CTACAAGAAGCGCACCCGTCGC

Protein sequence

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MDPQPEPVSYICGDCGMENTLKQGDVIQCRECGYRILYKKRTRR

MC03g1133 (gene) of Bitter gourd (Dali-11) v1 genome